Longitudinal Comparison of Bacterial Diversity and Antibiotic Resistance Genes in New York City Sewage

ABSTRACT Bacterial resistance to antibiotics is a pressing health issue around the world, not only in health care settings but also in the community and environment, particularly in crowded urban populations. The aim of our work was to characterize the microbial populations in sewage and the spread...

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Autores principales:	Susan M. Joseph, Thomas Battaglia, Julia M. Maritz, Jane M. Carlton, Martin J. Blaser
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2019
Materias:	antibiotic resistance microbiome New York City sewage Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/4a8ae4f4fa5249b4a5c0006831bce7c4
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spelling	oai:doaj.org-article:4a8ae4f4fa5249b4a5c0006831bce7c42021-12-02T18:25:16ZLongitudinal Comparison of Bacterial Diversity and Antibiotic Resistance Genes in New York City Sewage10.1128/mSystems.00327-192379-5077https://doaj.org/article/4a8ae4f4fa5249b4a5c0006831bce7c42019-08-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/mSystems.00327-19https://doaj.org/toc/2379-5077ABSTRACT Bacterial resistance to antibiotics is a pressing health issue around the world, not only in health care settings but also in the community and environment, particularly in crowded urban populations. The aim of our work was to characterize the microbial populations in sewage and the spread of antibiotic resistance within New York City (NYC). Here, we investigated the structure of the microbiome and the prevalence of antibiotic resistance genes in raw sewage samples collected from the fourteen NYC Department of Environmental Protection wastewater treatment plants, distributed across the five NYC boroughs. Sewage, a direct output of anthropogenic activity and a reservoir of microbes, provides an ecological niche to examine the spread of antibiotic resistance. Taxonomic diversity analysis revealed a largely similar and stable bacterial population structure across all the samples, which was found to be similar over three time points in an annual cycle, as well as in the five NYC boroughs. All samples were positive for the presence of the seven antibiotic resistance genes tested, based on real-time quantitative PCR assays, with higher levels observed for tetracycline resistance genes at all time points. For five of the seven genes, abundances were significantly higher in May than in February and August. This study provides characteristics of the NYC sewage resistome in the context of the overall bacterial populations. IMPORTANCE Urban sewage or wastewater is a diverse source of bacterial growth, as well as a hot spot for the development of environmental antibiotic resistance, which can in turn influence the health of the residents of the city. As part of a larger study to characterize the urban New York City microbial metagenome, we collected raw sewage samples representing three seasonal time points spanning the five boroughs of NYC and went on to characterize the microbiome and the presence of a range of antibiotic resistance genes. Through this study, we have established a baseline microbial population and antibiotic resistance abundance in NYC sewage which can prove to be very useful in studying the load of antibiotic usage, as well as for developing effective measures in antibiotic stewardship.Susan M. JosephThomas BattagliaJulia M. MaritzJane M. CarltonMartin J. BlaserAmerican Society for Microbiologyarticleantibiotic resistancemicrobiomeNew York CitysewageMicrobiologyQR1-502ENmSystems, Vol 4, Iss 4 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	antibiotic resistance microbiome New York City sewage Microbiology QR1-502
spellingShingle	antibiotic resistance microbiome New York City sewage Microbiology QR1-502 Susan M. Joseph Thomas Battaglia Julia M. Maritz Jane M. Carlton Martin J. Blaser Longitudinal Comparison of Bacterial Diversity and Antibiotic Resistance Genes in New York City Sewage
description	ABSTRACT Bacterial resistance to antibiotics is a pressing health issue around the world, not only in health care settings but also in the community and environment, particularly in crowded urban populations. The aim of our work was to characterize the microbial populations in sewage and the spread of antibiotic resistance within New York City (NYC). Here, we investigated the structure of the microbiome and the prevalence of antibiotic resistance genes in raw sewage samples collected from the fourteen NYC Department of Environmental Protection wastewater treatment plants, distributed across the five NYC boroughs. Sewage, a direct output of anthropogenic activity and a reservoir of microbes, provides an ecological niche to examine the spread of antibiotic resistance. Taxonomic diversity analysis revealed a largely similar and stable bacterial population structure across all the samples, which was found to be similar over three time points in an annual cycle, as well as in the five NYC boroughs. All samples were positive for the presence of the seven antibiotic resistance genes tested, based on real-time quantitative PCR assays, with higher levels observed for tetracycline resistance genes at all time points. For five of the seven genes, abundances were significantly higher in May than in February and August. This study provides characteristics of the NYC sewage resistome in the context of the overall bacterial populations. IMPORTANCE Urban sewage or wastewater is a diverse source of bacterial growth, as well as a hot spot for the development of environmental antibiotic resistance, which can in turn influence the health of the residents of the city. As part of a larger study to characterize the urban New York City microbial metagenome, we collected raw sewage samples representing three seasonal time points spanning the five boroughs of NYC and went on to characterize the microbiome and the presence of a range of antibiotic resistance genes. Through this study, we have established a baseline microbial population and antibiotic resistance abundance in NYC sewage which can prove to be very useful in studying the load of antibiotic usage, as well as for developing effective measures in antibiotic stewardship.
format	article
author	Susan M. Joseph Thomas Battaglia Julia M. Maritz Jane M. Carlton Martin J. Blaser
author_facet	Susan M. Joseph Thomas Battaglia Julia M. Maritz Jane M. Carlton Martin J. Blaser
author_sort	Susan M. Joseph
title	Longitudinal Comparison of Bacterial Diversity and Antibiotic Resistance Genes in New York City Sewage
title_short	Longitudinal Comparison of Bacterial Diversity and Antibiotic Resistance Genes in New York City Sewage
title_full	Longitudinal Comparison of Bacterial Diversity and Antibiotic Resistance Genes in New York City Sewage
title_fullStr	Longitudinal Comparison of Bacterial Diversity and Antibiotic Resistance Genes in New York City Sewage
title_full_unstemmed	Longitudinal Comparison of Bacterial Diversity and Antibiotic Resistance Genes in New York City Sewage
title_sort	longitudinal comparison of bacterial diversity and antibiotic resistance genes in new york city sewage
publisher	American Society for Microbiology
publishDate	2019
url	https://doaj.org/article/4a8ae4f4fa5249b4a5c0006831bce7c4
work_keys_str_mv	AT susanmjoseph longitudinalcomparisonofbacterialdiversityandantibioticresistancegenesinnewyorkcitysewage AT thomasbattaglia longitudinalcomparisonofbacterialdiversityandantibioticresistancegenesinnewyorkcitysewage AT juliammaritz longitudinalcomparisonofbacterialdiversityandantibioticresistancegenesinnewyorkcitysewage AT janemcarlton longitudinalcomparisonofbacterialdiversityandantibioticresistancegenesinnewyorkcitysewage AT martinjblaser longitudinalcomparisonofbacterialdiversityandantibioticresistancegenesinnewyorkcitysewage
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Longitudinal Comparison of Bacterial Diversity and Antibiotic Resistance Genes in New York City Sewage

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