Phylogenetic comparison and splice site conservation of eukaryotic U1 snRNP-specific U1-70K gene family

Abstract Eukaryotic cells can expand their coding ability by using their splicing machinery, spliceosome, to process precursor mRNA (pre-mRNA) into mature messenger RNA. The mega-macromolecular spliceosome contains multiple subcomplexes, referred to as small nuclear ribonucleoproteins (snRNPs). Amon...

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Autores principales:	Tao Fan, Yu-Zhen Zhao, Jing-Fang Yang, Qin-Lai Liu, Yuan Tian, Das Debatosh, Ying-Gao Liu, Jianhua Zhang, Chen Chen, Mo-Xian Chen, Shao-Ming Zhou
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/4a8dd14d6d6743df8c4fbeb531d002aa
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spelling	oai:doaj.org-article:4a8dd14d6d6743df8c4fbeb531d002aa2021-12-02T17:41:26ZPhylogenetic comparison and splice site conservation of eukaryotic U1 snRNP-specific U1-70K gene family10.1038/s41598-021-91693-32045-2322https://doaj.org/article/4a8dd14d6d6743df8c4fbeb531d002aa2021-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-91693-3https://doaj.org/toc/2045-2322Abstract Eukaryotic cells can expand their coding ability by using their splicing machinery, spliceosome, to process precursor mRNA (pre-mRNA) into mature messenger RNA. The mega-macromolecular spliceosome contains multiple subcomplexes, referred to as small nuclear ribonucleoproteins (snRNPs). Among these, U1 snRNP and its central component, U1-70K, are crucial for splice site recognition during early spliceosome assembly. The human U1-70K has been linked to several types of human autoimmune and neurodegenerative diseases. However, its phylogenetic relationship has been seldom reported. To this end, we carried out a systemic analysis of 95 animal U1-70K genes and compare these proteins to their yeast and plant counterparts. Analysis of their gene and protein structures, expression patterns and splicing conservation suggest that animal U1-70Ks are conserved in their molecular function, and may play essential role in cancers and juvenile development. In particular, animal U1-70Ks display unique characteristics of single copy number and a splicing isoform with truncated C-terminal, suggesting the specific role of these U1-70Ks in animal kingdom. In summary, our results provide phylogenetic overview of U1-70K gene family in vertebrates. In silico analyses conducted in this work will act as a reference for future functional studies of this crucial U1 splicing factor in animal kingdom.Tao FanYu-Zhen ZhaoJing-Fang YangQin-Lai LiuYuan TianDas DebatoshYing-Gao LiuJianhua ZhangChen ChenMo-Xian ChenShao-Ming ZhouNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-15 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Tao Fan Yu-Zhen Zhao Jing-Fang Yang Qin-Lai Liu Yuan Tian Das Debatosh Ying-Gao Liu Jianhua Zhang Chen Chen Mo-Xian Chen Shao-Ming Zhou Phylogenetic comparison and splice site conservation of eukaryotic U1 snRNP-specific U1-70K gene family
description	Abstract Eukaryotic cells can expand their coding ability by using their splicing machinery, spliceosome, to process precursor mRNA (pre-mRNA) into mature messenger RNA. The mega-macromolecular spliceosome contains multiple subcomplexes, referred to as small nuclear ribonucleoproteins (snRNPs). Among these, U1 snRNP and its central component, U1-70K, are crucial for splice site recognition during early spliceosome assembly. The human U1-70K has been linked to several types of human autoimmune and neurodegenerative diseases. However, its phylogenetic relationship has been seldom reported. To this end, we carried out a systemic analysis of 95 animal U1-70K genes and compare these proteins to their yeast and plant counterparts. Analysis of their gene and protein structures, expression patterns and splicing conservation suggest that animal U1-70Ks are conserved in their molecular function, and may play essential role in cancers and juvenile development. In particular, animal U1-70Ks display unique characteristics of single copy number and a splicing isoform with truncated C-terminal, suggesting the specific role of these U1-70Ks in animal kingdom. In summary, our results provide phylogenetic overview of U1-70K gene family in vertebrates. In silico analyses conducted in this work will act as a reference for future functional studies of this crucial U1 splicing factor in animal kingdom.
format	article
author	Tao Fan Yu-Zhen Zhao Jing-Fang Yang Qin-Lai Liu Yuan Tian Das Debatosh Ying-Gao Liu Jianhua Zhang Chen Chen Mo-Xian Chen Shao-Ming Zhou
author_facet	Tao Fan Yu-Zhen Zhao Jing-Fang Yang Qin-Lai Liu Yuan Tian Das Debatosh Ying-Gao Liu Jianhua Zhang Chen Chen Mo-Xian Chen Shao-Ming Zhou
author_sort	Tao Fan
title	Phylogenetic comparison and splice site conservation of eukaryotic U1 snRNP-specific U1-70K gene family
title_short	Phylogenetic comparison and splice site conservation of eukaryotic U1 snRNP-specific U1-70K gene family
title_full	Phylogenetic comparison and splice site conservation of eukaryotic U1 snRNP-specific U1-70K gene family
title_fullStr	Phylogenetic comparison and splice site conservation of eukaryotic U1 snRNP-specific U1-70K gene family
title_full_unstemmed	Phylogenetic comparison and splice site conservation of eukaryotic U1 snRNP-specific U1-70K gene family
title_sort	phylogenetic comparison and splice site conservation of eukaryotic u1 snrnp-specific u1-70k gene family
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/4a8dd14d6d6743df8c4fbeb531d002aa
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Phylogenetic comparison and splice site conservation of eukaryotic U1 snRNP-specific U1-70K gene family

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