Mir-26b inhibits growth and resistance to paclitaxel chemotherapy by silencing the CDC6 gene in gastric cancer

Introduction Gastric cancer is one of the most common cancers of the digestive system and is associated with high morbidity and mortality. The aim of this study was to investigate whether miR-26b is involved in the proliferation and resistance to paclitaxel chemotherapy in gastric cancer cells. Mat...

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Autores principales:	Bochao Zhao, Jiale Zhang, Xiuxiu Chen, Huimian Xu, Baojun Huang
Formato:	article
Lenguaje:	EN
Publicado:	Termedia Publishing House 2019
Materias:	chemotherapy gastric cancer paclitaxel mir-26b cdc6 Medicine R
Acceso en línea:	https://doaj.org/article/4a91ffd2a37d44fabec2645477934679
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spelling	oai:doaj.org-article:4a91ffd2a37d44fabec26454779346792021-12-02T18:33:05ZMir-26b inhibits growth and resistance to paclitaxel chemotherapy by silencing the CDC6 gene in gastric cancer1734-19221896-915110.5114/aoms.2018.73315https://doaj.org/article/4a91ffd2a37d44fabec26454779346792019-03-01T00:00:00Zhttps://www.archivesofmedicalscience.com/Mir-26b-inhibits-growth-and-resistance-to-paclitaxel-chemotherapy-by-silencing-the,69140,0,2.htmlhttps://doaj.org/toc/1734-1922https://doaj.org/toc/1896-9151Introduction Gastric cancer is one of the most common cancers of the digestive system and is associated with high morbidity and mortality. The aim of this study was to investigate whether miR-26b is involved in the proliferation and resistance to paclitaxel chemotherapy in gastric cancer cells. Material and methods The expression of miR-26b in gastric cancer cell lines was determined by quantitative real-time PCR. Bioinformatics software was used to predict potential target genes of miR-26b. Luciferase assay was used to verify the interactions between target genes and miR-26b. CDC6 protein expression was measured by Western blot. The proliferation and chemotherapy resistance were analyzed by MTT assay. Cell invasion was evaluated by Transwell assay. Results MiR-26b was down-regulated in gastric cancer cell lines compared to normal control cells, and its expression in drug resistance cells was even lower (p < 0.05). CDC6 was identified as a potential target gene of miR-26b by using bioinformatics analysis software. The expression of CDC6 was inhibited by miR-26b both at RNA level, which was determined by luciferase assay, and at protein level, which was determined by Western blot (p < 0.05). Silencing CDC6 inhibited cell proliferation, invasion, and promoted apoptosis of gastric cancer cell lines, BGC823 and SGC7901 (p < 0.05). Moreover, CDC6 knockdown inhibited chemotherapy resistance to paclitaxel, IC50 to paclitaxel decreased from 153.17 ±0.49 µg/l to 39.81 ±0.28 µg/l (p < 0.05). Conclusions miR-26b inhibits growth and resistance to paclitaxel chemotherapy by silencing the CDC6 gene in the gastric cancer cell line SGC7901.Bochao ZhaoJiale ZhangXiuxiu ChenHuimian XuBaojun HuangTermedia Publishing Housearticlechemotherapygastric cancerpaclitaxelmir-26bcdc6gastric cancerchemotherapypaclitaxelMedicineRENArchives of Medical Science, Vol 15, Iss 2, Pp 498-503 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	chemotherapy gastric cancer paclitaxel mir-26b cdc6 gastric cancer chemotherapy paclitaxel Medicine R
spellingShingle	chemotherapy gastric cancer paclitaxel mir-26b cdc6 gastric cancer chemotherapy paclitaxel Medicine R Bochao Zhao Jiale Zhang Xiuxiu Chen Huimian Xu Baojun Huang Mir-26b inhibits growth and resistance to paclitaxel chemotherapy by silencing the CDC6 gene in gastric cancer
description	Introduction Gastric cancer is one of the most common cancers of the digestive system and is associated with high morbidity and mortality. The aim of this study was to investigate whether miR-26b is involved in the proliferation and resistance to paclitaxel chemotherapy in gastric cancer cells. Material and methods The expression of miR-26b in gastric cancer cell lines was determined by quantitative real-time PCR. Bioinformatics software was used to predict potential target genes of miR-26b. Luciferase assay was used to verify the interactions between target genes and miR-26b. CDC6 protein expression was measured by Western blot. The proliferation and chemotherapy resistance were analyzed by MTT assay. Cell invasion was evaluated by Transwell assay. Results MiR-26b was down-regulated in gastric cancer cell lines compared to normal control cells, and its expression in drug resistance cells was even lower (p < 0.05). CDC6 was identified as a potential target gene of miR-26b by using bioinformatics analysis software. The expression of CDC6 was inhibited by miR-26b both at RNA level, which was determined by luciferase assay, and at protein level, which was determined by Western blot (p < 0.05). Silencing CDC6 inhibited cell proliferation, invasion, and promoted apoptosis of gastric cancer cell lines, BGC823 and SGC7901 (p < 0.05). Moreover, CDC6 knockdown inhibited chemotherapy resistance to paclitaxel, IC50 to paclitaxel decreased from 153.17 ±0.49 µg/l to 39.81 ±0.28 µg/l (p < 0.05). Conclusions miR-26b inhibits growth and resistance to paclitaxel chemotherapy by silencing the CDC6 gene in the gastric cancer cell line SGC7901.
format	article
author	Bochao Zhao Jiale Zhang Xiuxiu Chen Huimian Xu Baojun Huang
author_facet	Bochao Zhao Jiale Zhang Xiuxiu Chen Huimian Xu Baojun Huang
author_sort	Bochao Zhao
title	Mir-26b inhibits growth and resistance to paclitaxel chemotherapy by silencing the CDC6 gene in gastric cancer
title_short	Mir-26b inhibits growth and resistance to paclitaxel chemotherapy by silencing the CDC6 gene in gastric cancer
title_full	Mir-26b inhibits growth and resistance to paclitaxel chemotherapy by silencing the CDC6 gene in gastric cancer
title_fullStr	Mir-26b inhibits growth and resistance to paclitaxel chemotherapy by silencing the CDC6 gene in gastric cancer
title_full_unstemmed	Mir-26b inhibits growth and resistance to paclitaxel chemotherapy by silencing the CDC6 gene in gastric cancer
title_sort	mir-26b inhibits growth and resistance to paclitaxel chemotherapy by silencing the cdc6 gene in gastric cancer
publisher	Termedia Publishing House
publishDate	2019
url	https://doaj.org/article/4a91ffd2a37d44fabec2645477934679
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Mir-26b inhibits growth and resistance to paclitaxel chemotherapy by silencing the CDC6 gene in gastric cancer

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