Utility of total cell-free DNA levels for surgical damage evaluation in patients with urological surgeries

Abstract The evaluation of surgical damage is challenging because of the lack of specific biomarkers. Total cell-free DNA (cfDNA) levels have been reported to increase with external trauma and may be a biomarker for tissue damage. To investigate the utility of perioperative total cfDNA levels in eva...

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Autores principales:	Sakae Konishi, Takuma Narita, Shingo Hatakeyama, Tohru Yoneyama, Mihoko Sutoh Yoneyama, Yuki Tobisawa, Daisuke Noro, Tendo Sato, Kyo Togashi, Teppei Okamoto, Hayato Yamamoto, Takahiro Yoneyama, Yasuhiro Hashimoto, Chikara Ohyama
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/4a938730a3884aa8aee20ccb5e394ecc
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spelling	oai:doaj.org-article:4a938730a3884aa8aee20ccb5e394ecc2021-11-14T12:20:46ZUtility of total cell-free DNA levels for surgical damage evaluation in patients with urological surgeries10.1038/s41598-021-01430-z2045-2322https://doaj.org/article/4a938730a3884aa8aee20ccb5e394ecc2021-11-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-01430-zhttps://doaj.org/toc/2045-2322Abstract The evaluation of surgical damage is challenging because of the lack of specific biomarkers. Total cell-free DNA (cfDNA) levels have been reported to increase with external trauma and may be a biomarker for tissue damage. To investigate the utility of perioperative total cfDNA levels in evaluating surgical damage in urological surgeries. This multicenter, prospective, observational study included 196 patients scheduled for urological surgeries between September 2020 and July 2021. The primary outcome was the change in total cfDNA levels before and after urological surgery. The secondary outcome was the effect of surgical type on total cfDNA ratio before and after urological surgery. The postoperative median total cfDNA level of the 196 patients was significantly increased 2.5-fold compared to the preoperative level (185.2 ng/mL vs. 406.7 ng/mL, P < 0.001). The median total cfDNA before/after ratio was greater than four-fold for kidney transplantation, open cystectomy, and open adrenalectomy. The ratio was less than two-fold for laparoscopic adrenalectomy and robot-assisted radical prostatectomy. Major surgery showed a significant postoperative increase in total cfDNA levels, while minor surgery did not. Total cfDNA levels increased 2.5-fold after urological surgery and it can be used as an acute-phase biomarker for surgical damage.Sakae KonishiTakuma NaritaShingo HatakeyamaTohru YoneyamaMihoko Sutoh YoneyamaYuki TobisawaDaisuke NoroTendo SatoKyo TogashiTeppei OkamotoHayato YamamotoTakahiro YoneyamaYasuhiro HashimotoChikara OhyamaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-8 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Sakae Konishi Takuma Narita Shingo Hatakeyama Tohru Yoneyama Mihoko Sutoh Yoneyama Yuki Tobisawa Daisuke Noro Tendo Sato Kyo Togashi Teppei Okamoto Hayato Yamamoto Takahiro Yoneyama Yasuhiro Hashimoto Chikara Ohyama Utility of total cell-free DNA levels for surgical damage evaluation in patients with urological surgeries
description	Abstract The evaluation of surgical damage is challenging because of the lack of specific biomarkers. Total cell-free DNA (cfDNA) levels have been reported to increase with external trauma and may be a biomarker for tissue damage. To investigate the utility of perioperative total cfDNA levels in evaluating surgical damage in urological surgeries. This multicenter, prospective, observational study included 196 patients scheduled for urological surgeries between September 2020 and July 2021. The primary outcome was the change in total cfDNA levels before and after urological surgery. The secondary outcome was the effect of surgical type on total cfDNA ratio before and after urological surgery. The postoperative median total cfDNA level of the 196 patients was significantly increased 2.5-fold compared to the preoperative level (185.2 ng/mL vs. 406.7 ng/mL, P < 0.001). The median total cfDNA before/after ratio was greater than four-fold for kidney transplantation, open cystectomy, and open adrenalectomy. The ratio was less than two-fold for laparoscopic adrenalectomy and robot-assisted radical prostatectomy. Major surgery showed a significant postoperative increase in total cfDNA levels, while minor surgery did not. Total cfDNA levels increased 2.5-fold after urological surgery and it can be used as an acute-phase biomarker for surgical damage.
format	article
author	Sakae Konishi Takuma Narita Shingo Hatakeyama Tohru Yoneyama Mihoko Sutoh Yoneyama Yuki Tobisawa Daisuke Noro Tendo Sato Kyo Togashi Teppei Okamoto Hayato Yamamoto Takahiro Yoneyama Yasuhiro Hashimoto Chikara Ohyama
author_facet	Sakae Konishi Takuma Narita Shingo Hatakeyama Tohru Yoneyama Mihoko Sutoh Yoneyama Yuki Tobisawa Daisuke Noro Tendo Sato Kyo Togashi Teppei Okamoto Hayato Yamamoto Takahiro Yoneyama Yasuhiro Hashimoto Chikara Ohyama
author_sort	Sakae Konishi
title	Utility of total cell-free DNA levels for surgical damage evaluation in patients with urological surgeries
title_short	Utility of total cell-free DNA levels for surgical damage evaluation in patients with urological surgeries
title_full	Utility of total cell-free DNA levels for surgical damage evaluation in patients with urological surgeries
title_fullStr	Utility of total cell-free DNA levels for surgical damage evaluation in patients with urological surgeries
title_full_unstemmed	Utility of total cell-free DNA levels for surgical damage evaluation in patients with urological surgeries
title_sort	utility of total cell-free dna levels for surgical damage evaluation in patients with urological surgeries
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/4a938730a3884aa8aee20ccb5e394ecc
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Utility of total cell-free DNA levels for surgical damage evaluation in patients with urological surgeries

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