Antioxidative fullerol promotes osteogenesis of human adipose-derived stem cells

Xinlin Yang, Ching-Ju Li, Yueping Wan, Pinar Smith, Guowei Shang, Quanjun Cui Department of Orthopaedic Surgery, University of Virginia School of Medicine, Charlottesville, VA, USA Abstract: Antioxidants were implicated as potential reagents to enhance osteogenesis, and nano-fullerenes have been...

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Autores principales:	Yang XL, Li CJ, Wan YP, Smith P, Shang GW, Cui Q
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2014
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/4a9bb2844a0e43dfa7a7effe98465db7
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spelling	oai:doaj.org-article:4a9bb2844a0e43dfa7a7effe98465db72021-12-02T01:30:30ZAntioxidative fullerol promotes osteogenesis of human adipose-derived stem cells1178-2013https://doaj.org/article/4a9bb2844a0e43dfa7a7effe98465db72014-08-01T00:00:00Zhttp://www.dovepress.com/antioxidative-fullerol-promotes-osteogenesis-of-human-adipose-derived--peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013 Xinlin Yang, Ching-Ju Li, Yueping Wan, Pinar Smith, Guowei Shang, Quanjun Cui Department of Orthopaedic Surgery, University of Virginia School of Medicine, Charlottesville, VA, USA Abstract: Antioxidants were implicated as potential reagents to enhance osteogenesis, and nano-fullerenes have been demonstrated to have a great antioxidative capacity by both in vitro and in vivo experiments. In this study, we assessed the impact of a polyhydroxylated fullerene, fullerol, on the osteogenic differentiation of human adipose-derived stem cells (ADSCs). Fullerol was not toxic against human ADSCs at concentrations up to 10 µM. At a concentration of 1 µM, fullerol reduced cellular reactive oxygen species after a 5-day incubation either in the presence or in the absence of osteogenic media. Pretreatment of fullerol for 7 days increased the osteogenic potential of human ADSCs. Furthermore, when incubated together with osteogenic medium, fullerol promoted osteogenic differentiation in a dose-dependent manner. Finally, fullerol proved to promote expression of FoxO1, a major functional isoform of forkhead box O transcription factors that defend against reactive oxygen species in bone. Although further clarification of related mechanisms is required, the findings may help further development of a novel approach for bone repair, using combined treatment of nano-fullerol with ADSCs. Keywords: polyhydroxylated fullerene, bone repair, reactive oxygen species, forkhead box protein O1Yang XLLi CJWan YPSmith PShang GWCui QDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2014, Iss Issue 1, Pp 4023-4031 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Yang XL Li CJ Wan YP Smith P Shang GW Cui Q Antioxidative fullerol promotes osteogenesis of human adipose-derived stem cells
description	Xinlin Yang, Ching-Ju Li, Yueping Wan, Pinar Smith, Guowei Shang, Quanjun Cui Department of Orthopaedic Surgery, University of Virginia School of Medicine, Charlottesville, VA, USA Abstract: Antioxidants were implicated as potential reagents to enhance osteogenesis, and nano-fullerenes have been demonstrated to have a great antioxidative capacity by both in vitro and in vivo experiments. In this study, we assessed the impact of a polyhydroxylated fullerene, fullerol, on the osteogenic differentiation of human adipose-derived stem cells (ADSCs). Fullerol was not toxic against human ADSCs at concentrations up to 10 µM. At a concentration of 1 µM, fullerol reduced cellular reactive oxygen species after a 5-day incubation either in the presence or in the absence of osteogenic media. Pretreatment of fullerol for 7 days increased the osteogenic potential of human ADSCs. Furthermore, when incubated together with osteogenic medium, fullerol promoted osteogenic differentiation in a dose-dependent manner. Finally, fullerol proved to promote expression of FoxO1, a major functional isoform of forkhead box O transcription factors that defend against reactive oxygen species in bone. Although further clarification of related mechanisms is required, the findings may help further development of a novel approach for bone repair, using combined treatment of nano-fullerol with ADSCs. Keywords: polyhydroxylated fullerene, bone repair, reactive oxygen species, forkhead box protein O1
format	article
author	Yang XL Li CJ Wan YP Smith P Shang GW Cui Q
author_facet	Yang XL Li CJ Wan YP Smith P Shang GW Cui Q
author_sort	Yang XL
title	Antioxidative fullerol promotes osteogenesis of human adipose-derived stem cells
title_short	Antioxidative fullerol promotes osteogenesis of human adipose-derived stem cells
title_full	Antioxidative fullerol promotes osteogenesis of human adipose-derived stem cells
title_fullStr	Antioxidative fullerol promotes osteogenesis of human adipose-derived stem cells
title_full_unstemmed	Antioxidative fullerol promotes osteogenesis of human adipose-derived stem cells
title_sort	antioxidative fullerol promotes osteogenesis of human adipose-derived stem cells
publisher	Dove Medical Press
publishDate	2014
url	https://doaj.org/article/4a9bb2844a0e43dfa7a7effe98465db7
work_keys_str_mv	AT yangxl antioxidativefullerolpromotesosteogenesisofhumanadiposederivedstemcells AT licj antioxidativefullerolpromotesosteogenesisofhumanadiposederivedstemcells AT wanyp antioxidativefullerolpromotesosteogenesisofhumanadiposederivedstemcells AT smithp antioxidativefullerolpromotesosteogenesisofhumanadiposederivedstemcells AT shanggw antioxidativefullerolpromotesosteogenesisofhumanadiposederivedstemcells AT cuiq antioxidativefullerolpromotesosteogenesisofhumanadiposederivedstemcells
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Antioxidative fullerol promotes osteogenesis of human adipose-derived stem cells

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