Determining seropositivity-A review of approaches to define population seroprevalence when using multiplex bead assays to assess burden of tropical diseases.

<h4>Background</h4>Serological surveys with multiplex bead assays can be used to assess seroprevalence to multiple pathogens simultaneously. However, multiple methods have been used to generate cut-off values for seropositivity and these may lead to inconsistent interpretation of results...

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Autores principales:	YuYen Chan, Kimberly Fornace, Lindsey Wu, Benjamin F Arnold, Jeffrey W Priest, Diana L Martin, Michelle A Chang, Jackie Cook, Gillian Stresman, Chris Drakeley
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270
Acceso en línea:	https://doaj.org/article/4a9e2f514c6b4d98a854ba6b3e61102a
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spelling	oai:doaj.org-article:4a9e2f514c6b4d98a854ba6b3e61102a2021-11-25T06:31:44ZDetermining seropositivity-A review of approaches to define population seroprevalence when using multiplex bead assays to assess burden of tropical diseases.1935-27271935-273510.1371/journal.pntd.0009457https://doaj.org/article/4a9e2f514c6b4d98a854ba6b3e61102a2021-06-01T00:00:00Zhttps://doi.org/10.1371/journal.pntd.0009457https://doaj.org/toc/1935-2727https://doaj.org/toc/1935-2735<h4>Background</h4>Serological surveys with multiplex bead assays can be used to assess seroprevalence to multiple pathogens simultaneously. However, multiple methods have been used to generate cut-off values for seropositivity and these may lead to inconsistent interpretation of results. A literature review was conducted to describe the methods used to determine cut-off values for data generated by multiplex bead assays.<h4>Methodology/principal findings</h4>A search was conducted in PubMed that included articles published from January 2010 to January 2020, and 308 relevant articles were identified that included the terms "serology", "cut-offs", and "multiplex bead assays". After application of exclusion of articles not relevant to neglected tropical diseases (NTD), vaccine preventable diseases (VPD), or malaria, 55 articles were examined based on their relevance to NTD or VPD. The most frequently applied approaches to determine seropositivity included the use of presumed unexposed populations, mixture models, receiver operating curves (ROC), and international standards. Other methods included the use of quantiles, pre-exposed endemic cohorts, and visual inflection points.<h4>Conclusions/significance</h4>For disease control programmes, seropositivity is a practical and easily interpretable health metric but determining appropriate cut-offs for positivity can be challenging. Considerations for optimal cut-off approaches should include factors such as methods recommended by previous research, transmission dynamics, and the immunological backgrounds of the population. In the absence of international standards for estimating seropositivity in a population, the use of consistent methods that align with individual disease epidemiological data will improve comparability between settings and enable the assessment of changes over time.YuYen ChanKimberly FornaceLindsey WuBenjamin F ArnoldJeffrey W PriestDiana L MartinMichelle A ChangJackie CookGillian StresmanChris DrakeleyPublic Library of Science (PLoS)articleArctic medicine. Tropical medicineRC955-962Public aspects of medicineRA1-1270ENPLoS Neglected Tropical Diseases, Vol 15, Iss 6, p e0009457 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270
spellingShingle	Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 YuYen Chan Kimberly Fornace Lindsey Wu Benjamin F Arnold Jeffrey W Priest Diana L Martin Michelle A Chang Jackie Cook Gillian Stresman Chris Drakeley Determining seropositivity-A review of approaches to define population seroprevalence when using multiplex bead assays to assess burden of tropical diseases.
description	<h4>Background</h4>Serological surveys with multiplex bead assays can be used to assess seroprevalence to multiple pathogens simultaneously. However, multiple methods have been used to generate cut-off values for seropositivity and these may lead to inconsistent interpretation of results. A literature review was conducted to describe the methods used to determine cut-off values for data generated by multiplex bead assays.<h4>Methodology/principal findings</h4>A search was conducted in PubMed that included articles published from January 2010 to January 2020, and 308 relevant articles were identified that included the terms "serology", "cut-offs", and "multiplex bead assays". After application of exclusion of articles not relevant to neglected tropical diseases (NTD), vaccine preventable diseases (VPD), or malaria, 55 articles were examined based on their relevance to NTD or VPD. The most frequently applied approaches to determine seropositivity included the use of presumed unexposed populations, mixture models, receiver operating curves (ROC), and international standards. Other methods included the use of quantiles, pre-exposed endemic cohorts, and visual inflection points.<h4>Conclusions/significance</h4>For disease control programmes, seropositivity is a practical and easily interpretable health metric but determining appropriate cut-offs for positivity can be challenging. Considerations for optimal cut-off approaches should include factors such as methods recommended by previous research, transmission dynamics, and the immunological backgrounds of the population. In the absence of international standards for estimating seropositivity in a population, the use of consistent methods that align with individual disease epidemiological data will improve comparability between settings and enable the assessment of changes over time.
format	article
author	YuYen Chan Kimberly Fornace Lindsey Wu Benjamin F Arnold Jeffrey W Priest Diana L Martin Michelle A Chang Jackie Cook Gillian Stresman Chris Drakeley
author_facet	YuYen Chan Kimberly Fornace Lindsey Wu Benjamin F Arnold Jeffrey W Priest Diana L Martin Michelle A Chang Jackie Cook Gillian Stresman Chris Drakeley
author_sort	YuYen Chan
title	Determining seropositivity-A review of approaches to define population seroprevalence when using multiplex bead assays to assess burden of tropical diseases.
title_short	Determining seropositivity-A review of approaches to define population seroprevalence when using multiplex bead assays to assess burden of tropical diseases.
title_full	Determining seropositivity-A review of approaches to define population seroprevalence when using multiplex bead assays to assess burden of tropical diseases.
title_fullStr	Determining seropositivity-A review of approaches to define population seroprevalence when using multiplex bead assays to assess burden of tropical diseases.
title_full_unstemmed	Determining seropositivity-A review of approaches to define population seroprevalence when using multiplex bead assays to assess burden of tropical diseases.
title_sort	determining seropositivity-a review of approaches to define population seroprevalence when using multiplex bead assays to assess burden of tropical diseases.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/4a9e2f514c6b4d98a854ba6b3e61102a
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Determining seropositivity-A review of approaches to define population seroprevalence when using multiplex bead assays to assess burden of tropical diseases.

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