Network and role analysis of autophagy in Phytophthora sojae

Abstract Autophagy is an evolutionarily conserved mechanism in eukaryotes with roles in development and the virulence of plant fungal pathogens. However, few reports on autophagy in oomycete species have been published. Here, we identified 26 autophagy-related genes (ATGs) belonging to 20 different...

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Autores principales:	Linlin Chen, Xiong Zhang, Wen Wang, Xuejing Geng, Yan Shi, Risong Na, Daolong Dou, Honglian Li
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/4ac023e6d68448ca96a8e809439dd38a
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spelling	oai:doaj.org-article:4ac023e6d68448ca96a8e809439dd38a2021-12-02T12:30:43ZNetwork and role analysis of autophagy in Phytophthora sojae10.1038/s41598-017-01988-72045-2322https://doaj.org/article/4ac023e6d68448ca96a8e809439dd38a2017-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-01988-7https://doaj.org/toc/2045-2322Abstract Autophagy is an evolutionarily conserved mechanism in eukaryotes with roles in development and the virulence of plant fungal pathogens. However, few reports on autophagy in oomycete species have been published. Here, we identified 26 autophagy-related genes (ATGs) belonging to 20 different groups in Phytophthora sojae using a genome-wide survey, and core ATGs in oomycetes were used to construct a preliminary autophagy pathway model. Expression profile analysis revealed that these ATGs are broadly expressed and that the majority of them significantly increase during infection stages, suggesting a central role for autophagy in virulence. Autophagy in P. sojae was detected using a GFP-PsAtg8 fusion protein and the fluorescent dye MDC during rapamycin and starvation treatment. In addition, autophagy was significantly induced during sporangium formation and cyst germination. Silencing PsAtg6a in P. sojae significantly reduced sporulation and pathogenicity. Furthermore, a PsAtg6a-silenced strain showed haustorial formation defects. These results suggested that autophagy might play essential roles in both the development and infection mechanism of P. sojae.Linlin ChenXiong ZhangWen WangXuejing GengYan ShiRisong NaDaolong DouHonglian LiNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-12 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Linlin Chen Xiong Zhang Wen Wang Xuejing Geng Yan Shi Risong Na Daolong Dou Honglian Li Network and role analysis of autophagy in Phytophthora sojae
description	Abstract Autophagy is an evolutionarily conserved mechanism in eukaryotes with roles in development and the virulence of plant fungal pathogens. However, few reports on autophagy in oomycete species have been published. Here, we identified 26 autophagy-related genes (ATGs) belonging to 20 different groups in Phytophthora sojae using a genome-wide survey, and core ATGs in oomycetes were used to construct a preliminary autophagy pathway model. Expression profile analysis revealed that these ATGs are broadly expressed and that the majority of them significantly increase during infection stages, suggesting a central role for autophagy in virulence. Autophagy in P. sojae was detected using a GFP-PsAtg8 fusion protein and the fluorescent dye MDC during rapamycin and starvation treatment. In addition, autophagy was significantly induced during sporangium formation and cyst germination. Silencing PsAtg6a in P. sojae significantly reduced sporulation and pathogenicity. Furthermore, a PsAtg6a-silenced strain showed haustorial formation defects. These results suggested that autophagy might play essential roles in both the development and infection mechanism of P. sojae.
format	article
author	Linlin Chen Xiong Zhang Wen Wang Xuejing Geng Yan Shi Risong Na Daolong Dou Honglian Li
author_facet	Linlin Chen Xiong Zhang Wen Wang Xuejing Geng Yan Shi Risong Na Daolong Dou Honglian Li
author_sort	Linlin Chen
title	Network and role analysis of autophagy in Phytophthora sojae
title_short	Network and role analysis of autophagy in Phytophthora sojae
title_full	Network and role analysis of autophagy in Phytophthora sojae
title_fullStr	Network and role analysis of autophagy in Phytophthora sojae
title_full_unstemmed	Network and role analysis of autophagy in Phytophthora sojae
title_sort	network and role analysis of autophagy in phytophthora sojae
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/4ac023e6d68448ca96a8e809439dd38a
work_keys_str_mv	AT linlinchen networkandroleanalysisofautophagyinphytophthorasojae AT xiongzhang networkandroleanalysisofautophagyinphytophthorasojae AT wenwang networkandroleanalysisofautophagyinphytophthorasojae AT xuejinggeng networkandroleanalysisofautophagyinphytophthorasojae AT yanshi networkandroleanalysisofautophagyinphytophthorasojae AT risongna networkandroleanalysisofautophagyinphytophthorasojae AT daolongdou networkandroleanalysisofautophagyinphytophthorasojae AT honglianli networkandroleanalysisofautophagyinphytophthorasojae
_version_	1718394332469264384

Network and role analysis of autophagy in Phytophthora sojae

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