Live birth from slow-frozen rabbit oocytes after in vivo fertilisation.

In vivo fertilisation techniques such as intraoviductal oocyte transfer have been considered as alternatives to bypass the inadequacy of conventional in vitro fertilisation in rabbit. There is only one study in the literature, published in 1989, that reports live offspring from cryopreserved rabbit...

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Autores principales:	Estrella Jiménez-Trigos, José S Vicente, Francisco Marco-Jiménez
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/4ac4713c3839453195689ac83564f32e
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spelling	oai:doaj.org-article:4ac4713c3839453195689ac83564f32e2021-11-18T08:41:35ZLive birth from slow-frozen rabbit oocytes after in vivo fertilisation.1932-620310.1371/journal.pone.0083399https://doaj.org/article/4ac4713c3839453195689ac83564f32e2013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24358281/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203In vivo fertilisation techniques such as intraoviductal oocyte transfer have been considered as alternatives to bypass the inadequacy of conventional in vitro fertilisation in rabbit. There is only one study in the literature, published in 1989, that reports live offspring from cryopreserved rabbit oocytes. The aim of the present study was to establish the in vivo fertilisation procedure to generate live offspring with frozen oocytes. First, the effect of two recipient models (i) ovariectomised or (ii) oviduct ligated immediately after transfer on the ability of fresh oocytes to fertilise were compared. Second, generation of live offspring from slow-frozen oocytes was carried out using the ligated oviduct recipient model. Throughout the experiment, recipients were artificially inseminated 9 hours prior to oocyte transfer. In the first experiment, two days after unilateral transfer of fresh oocytes, oviducts and uterine horns were flushed to assess embryo recovery rates. The embryo recovery rates were low compared to control in both ovariectomised and ligated oviduct groups. However, ligated oviduct recipient showed significantly (P<0.05) higher embryo recovery rates compared to ovariectomised and control-transferred. In the second experiment, using bilateral oviduct ligation model, all females that received slow-frozen oocytes became pregnant and delivered a total of 4 live young naturally. Thus, in vivo fertilisation is an effective technique to generate live offspring using slow-frozen oocytes in rabbits.Estrella Jiménez-TrigosJosé S VicenteFrancisco Marco-JiménezPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 12, p e83399 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Estrella Jiménez-Trigos José S Vicente Francisco Marco-Jiménez Live birth from slow-frozen rabbit oocytes after in vivo fertilisation.
description	In vivo fertilisation techniques such as intraoviductal oocyte transfer have been considered as alternatives to bypass the inadequacy of conventional in vitro fertilisation in rabbit. There is only one study in the literature, published in 1989, that reports live offspring from cryopreserved rabbit oocytes. The aim of the present study was to establish the in vivo fertilisation procedure to generate live offspring with frozen oocytes. First, the effect of two recipient models (i) ovariectomised or (ii) oviduct ligated immediately after transfer on the ability of fresh oocytes to fertilise were compared. Second, generation of live offspring from slow-frozen oocytes was carried out using the ligated oviduct recipient model. Throughout the experiment, recipients were artificially inseminated 9 hours prior to oocyte transfer. In the first experiment, two days after unilateral transfer of fresh oocytes, oviducts and uterine horns were flushed to assess embryo recovery rates. The embryo recovery rates were low compared to control in both ovariectomised and ligated oviduct groups. However, ligated oviduct recipient showed significantly (P<0.05) higher embryo recovery rates compared to ovariectomised and control-transferred. In the second experiment, using bilateral oviduct ligation model, all females that received slow-frozen oocytes became pregnant and delivered a total of 4 live young naturally. Thus, in vivo fertilisation is an effective technique to generate live offspring using slow-frozen oocytes in rabbits.
format	article
author	Estrella Jiménez-Trigos José S Vicente Francisco Marco-Jiménez
author_facet	Estrella Jiménez-Trigos José S Vicente Francisco Marco-Jiménez
author_sort	Estrella Jiménez-Trigos
title	Live birth from slow-frozen rabbit oocytes after in vivo fertilisation.
title_short	Live birth from slow-frozen rabbit oocytes after in vivo fertilisation.
title_full	Live birth from slow-frozen rabbit oocytes after in vivo fertilisation.
title_fullStr	Live birth from slow-frozen rabbit oocytes after in vivo fertilisation.
title_full_unstemmed	Live birth from slow-frozen rabbit oocytes after in vivo fertilisation.
title_sort	live birth from slow-frozen rabbit oocytes after in vivo fertilisation.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/4ac4713c3839453195689ac83564f32e
work_keys_str_mv	AT estrellajimeneztrigos livebirthfromslowfrozenrabbitoocytesafterinvivofertilisation AT josesvicente livebirthfromslowfrozenrabbitoocytesafterinvivofertilisation AT franciscomarcojimenez livebirthfromslowfrozenrabbitoocytesafterinvivofertilisation
_version_	1718421443421667328

Live birth from slow-frozen rabbit oocytes after in vivo fertilisation.

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