Caveolin-1/Endothelial Nitric Oxide Synthase Interaction Is Reduced in Arteries From Pregnant Spontaneously Hypertensive Rats

We have investigated the role caveolae/caveolin-1 (Cav-1) plays in endothelial nitric oxide synthase (eNOS) activation and how it impacts pregnancy-induced decreased vascular reactivity in normotensive (Wistar rats) and spontaneously hypertensive rats (SHR). Wistar rats and SHR were divided into non...

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Autores principales:	Jéssica A. Troiano, Simone R. Potje, Murilo E. Graton, Emily T. Gonçalves, Rita C. Tostes, Cristina Antoniali
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	pregnancy spontaneously hypertensive rat aorta - thoracic mesenteric artery endothelial nitric oxide synthase caveolae Physiology QP1-981
Acceso en línea:	https://doaj.org/article/4ac62d10e25245b0b6aa86714d39f9f7
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spelling	oai:doaj.org-article:4ac62d10e25245b0b6aa86714d39f9f72021-11-09T16:57:52ZCaveolin-1/Endothelial Nitric Oxide Synthase Interaction Is Reduced in Arteries From Pregnant Spontaneously Hypertensive Rats1664-042X10.3389/fphys.2021.760237https://doaj.org/article/4ac62d10e25245b0b6aa86714d39f9f72021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fphys.2021.760237/fullhttps://doaj.org/toc/1664-042XWe have investigated the role caveolae/caveolin-1 (Cav-1) plays in endothelial nitric oxide synthase (eNOS) activation and how it impacts pregnancy-induced decreased vascular reactivity in normotensive (Wistar rats) and spontaneously hypertensive rats (SHR). Wistar rats and SHR were divided into non-pregnant (NP) and pregnant (P). Nitrite levels were assessed by the Griess method in the aorta and mesenteric vascular bed. In functional studies, arteries were incubated with methyl-β-cyclodextrin (dextrin, 10mmol/L), which disrupts caveolae by depleting cholesterol, and concentration-response curves to phenylephrine (PE) and acetylcholine (ACh) were constructed. Electronic microscopy was used to determine endothelial caveolae density in the aorta and resistance mesenteric artery in the presence of vehicle or dextrin (10mmol/L). Western blot was performed to evaluate Cav-1, p-Cav-1, calmodulin (CaM), and heat shock protein 90 (Hsp90) expression. Cav-1/eNOS interaction in the aorta and mesenteric vascular bed was assessed by co-immunoprecipitation. Nitric oxide (NO) generation was greater in arteries from P groups compared to NP groups. Dextrin did not change vascular responses in the aorta from P groups or the number of caveolae in P groups compared to NP groups. Compared to NP Wistar rats, NP SHR showed smaller number of caveolae and reduced Cav-1 expression. Pregnancy did not alter Cav-1, CaM, or Hsp90 expression in the aorta or mesenteric vascular bed from Wistar rats or SHR. These results suggest that pregnancy does not alter expression of the main eNOS regulatory proteins, but it decreases Cav-1/eNOS interaction. Reduced Cav-1/eNOS interaction in the aorta and mesenteric vascular bed seems to be an important mechanism to increase eNOS activity and nitric oxide production in pregnant normotensive and hypertensive rats.Jéssica A. TroianoJéssica A. TroianoSimone R. PotjeSimone R. PotjeMurilo E. GratonMurilo E. GratonEmily T. GonçalvesRita C. TostesCristina AntonialiCristina AntonialiFrontiers Media S.A.articlepregnancyspontaneously hypertensive rataorta - thoracicmesenteric arteryendothelial nitric oxide synthasecaveolaePhysiologyQP1-981ENFrontiers in Physiology, Vol 12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	pregnancy spontaneously hypertensive rat aorta - thoracic mesenteric artery endothelial nitric oxide synthase caveolae Physiology QP1-981
spellingShingle	pregnancy spontaneously hypertensive rat aorta - thoracic mesenteric artery endothelial nitric oxide synthase caveolae Physiology QP1-981 Jéssica A. Troiano Jéssica A. Troiano Simone R. Potje Simone R. Potje Murilo E. Graton Murilo E. Graton Emily T. Gonçalves Rita C. Tostes Cristina Antoniali Cristina Antoniali Caveolin-1/Endothelial Nitric Oxide Synthase Interaction Is Reduced in Arteries From Pregnant Spontaneously Hypertensive Rats
description	We have investigated the role caveolae/caveolin-1 (Cav-1) plays in endothelial nitric oxide synthase (eNOS) activation and how it impacts pregnancy-induced decreased vascular reactivity in normotensive (Wistar rats) and spontaneously hypertensive rats (SHR). Wistar rats and SHR were divided into non-pregnant (NP) and pregnant (P). Nitrite levels were assessed by the Griess method in the aorta and mesenteric vascular bed. In functional studies, arteries were incubated with methyl-β-cyclodextrin (dextrin, 10mmol/L), which disrupts caveolae by depleting cholesterol, and concentration-response curves to phenylephrine (PE) and acetylcholine (ACh) were constructed. Electronic microscopy was used to determine endothelial caveolae density in the aorta and resistance mesenteric artery in the presence of vehicle or dextrin (10mmol/L). Western blot was performed to evaluate Cav-1, p-Cav-1, calmodulin (CaM), and heat shock protein 90 (Hsp90) expression. Cav-1/eNOS interaction in the aorta and mesenteric vascular bed was assessed by co-immunoprecipitation. Nitric oxide (NO) generation was greater in arteries from P groups compared to NP groups. Dextrin did not change vascular responses in the aorta from P groups or the number of caveolae in P groups compared to NP groups. Compared to NP Wistar rats, NP SHR showed smaller number of caveolae and reduced Cav-1 expression. Pregnancy did not alter Cav-1, CaM, or Hsp90 expression in the aorta or mesenteric vascular bed from Wistar rats or SHR. These results suggest that pregnancy does not alter expression of the main eNOS regulatory proteins, but it decreases Cav-1/eNOS interaction. Reduced Cav-1/eNOS interaction in the aorta and mesenteric vascular bed seems to be an important mechanism to increase eNOS activity and nitric oxide production in pregnant normotensive and hypertensive rats.
format	article
author	Jéssica A. Troiano Jéssica A. Troiano Simone R. Potje Simone R. Potje Murilo E. Graton Murilo E. Graton Emily T. Gonçalves Rita C. Tostes Cristina Antoniali Cristina Antoniali
author_facet	Jéssica A. Troiano Jéssica A. Troiano Simone R. Potje Simone R. Potje Murilo E. Graton Murilo E. Graton Emily T. Gonçalves Rita C. Tostes Cristina Antoniali Cristina Antoniali
author_sort	Jéssica A. Troiano
title	Caveolin-1/Endothelial Nitric Oxide Synthase Interaction Is Reduced in Arteries From Pregnant Spontaneously Hypertensive Rats
title_short	Caveolin-1/Endothelial Nitric Oxide Synthase Interaction Is Reduced in Arteries From Pregnant Spontaneously Hypertensive Rats
title_full	Caveolin-1/Endothelial Nitric Oxide Synthase Interaction Is Reduced in Arteries From Pregnant Spontaneously Hypertensive Rats
title_fullStr	Caveolin-1/Endothelial Nitric Oxide Synthase Interaction Is Reduced in Arteries From Pregnant Spontaneously Hypertensive Rats
title_full_unstemmed	Caveolin-1/Endothelial Nitric Oxide Synthase Interaction Is Reduced in Arteries From Pregnant Spontaneously Hypertensive Rats
title_sort	caveolin-1/endothelial nitric oxide synthase interaction is reduced in arteries from pregnant spontaneously hypertensive rats
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/4ac62d10e25245b0b6aa86714d39f9f7
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Caveolin-1/Endothelial Nitric Oxide Synthase Interaction Is Reduced in Arteries From Pregnant Spontaneously Hypertensive Rats

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