Estrogens induce expression of membrane-associated estrogen receptor α isoforms in lactotropes.

Estrogens are key to anterior pituitary function, stimulating hormone release and controlling cell fate to achieve pituitary dynamic adaptation to changing physiological conditions. In addition to their classical mechanism of action through intracellular estrogen receptors (ERs), estrogens exert rap...

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Autores principales:	Sandra Zárate, Gabriela Jaita, Jimena Ferraris, Guadalupe Eijo, María L Magri, Daniel Pisera, Adriana Seilicovich
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/4ac7248c94134090a621c38474511624
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spelling	oai:doaj.org-article:4ac7248c94134090a621c384745116242021-11-18T07:11:33ZEstrogens induce expression of membrane-associated estrogen receptor α isoforms in lactotropes.1932-620310.1371/journal.pone.0041299https://doaj.org/article/4ac7248c94134090a621c384745116242012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22844453/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Estrogens are key to anterior pituitary function, stimulating hormone release and controlling cell fate to achieve pituitary dynamic adaptation to changing physiological conditions. In addition to their classical mechanism of action through intracellular estrogen receptors (ERs), estrogens exert rapid actions via cell membrane-localized ERs (mERs). We previously showed that E2 exerts a rapid pro-apoptotic action in anterior pituitary cells, especially in lactotropes and somatotropes, through activation of mERs. In the present study, we examined the involvement of mERα in the rapid pro-apoptotic action of estradiol by TUNEL in primary cultures of anterior pituitary cells from ovariectomized rats using a cell-impermeable E2 conjugate (E2-BSA) and an ERα selective antagonist (MPP dihydrochloride). We studied mERα expression during the estrous cycle and its regulation by gonadal steroids in vivo by flow cytometry. We identified ERα variants in the plasma membrane of anterior pituitary cells during the estrous cycle and studied E2 regulation of these mERα variants in vitro by surface biotinylation and Western Blot. E2-BSA-induced apoptosis was abrogated by MPP in total anterior pituitary cells and lactotropes. In cycling rats, we detected a higher number of lactotropes and a lower number of somatotropes expressing mERα at proestrus than at diestrus. Acute E2 treatment increased the percentage of mERα-expressing lactotropes whereas it decreased the percentage of mERα-expressing somatotropes. We detected three mERα isoforms of 66, 39 and 22 kDa. Expression of mERα66 and mERα39 was higher at proestrus than at diestrus, and short-term E2 incubation increased expression of these two mERα variants. Our results indicate that the rapid apoptotic action exerted by E2 in lactotropes depends on mERα, probably full-length ERα and/or a 39 kDa ERα variant. Expression and activation of mERα variants in lactotropes could be one of the mechanisms through which E2 participates in anterior pituitary cell renewal during the estrous cycle.Sandra ZárateGabriela JaitaJimena FerrarisGuadalupe EijoMaría L MagriDaniel PiseraAdriana SeilicovichPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 7, p e41299 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Sandra Zárate Gabriela Jaita Jimena Ferraris Guadalupe Eijo María L Magri Daniel Pisera Adriana Seilicovich Estrogens induce expression of membrane-associated estrogen receptor α isoforms in lactotropes.
description	Estrogens are key to anterior pituitary function, stimulating hormone release and controlling cell fate to achieve pituitary dynamic adaptation to changing physiological conditions. In addition to their classical mechanism of action through intracellular estrogen receptors (ERs), estrogens exert rapid actions via cell membrane-localized ERs (mERs). We previously showed that E2 exerts a rapid pro-apoptotic action in anterior pituitary cells, especially in lactotropes and somatotropes, through activation of mERs. In the present study, we examined the involvement of mERα in the rapid pro-apoptotic action of estradiol by TUNEL in primary cultures of anterior pituitary cells from ovariectomized rats using a cell-impermeable E2 conjugate (E2-BSA) and an ERα selective antagonist (MPP dihydrochloride). We studied mERα expression during the estrous cycle and its regulation by gonadal steroids in vivo by flow cytometry. We identified ERα variants in the plasma membrane of anterior pituitary cells during the estrous cycle and studied E2 regulation of these mERα variants in vitro by surface biotinylation and Western Blot. E2-BSA-induced apoptosis was abrogated by MPP in total anterior pituitary cells and lactotropes. In cycling rats, we detected a higher number of lactotropes and a lower number of somatotropes expressing mERα at proestrus than at diestrus. Acute E2 treatment increased the percentage of mERα-expressing lactotropes whereas it decreased the percentage of mERα-expressing somatotropes. We detected three mERα isoforms of 66, 39 and 22 kDa. Expression of mERα66 and mERα39 was higher at proestrus than at diestrus, and short-term E2 incubation increased expression of these two mERα variants. Our results indicate that the rapid apoptotic action exerted by E2 in lactotropes depends on mERα, probably full-length ERα and/or a 39 kDa ERα variant. Expression and activation of mERα variants in lactotropes could be one of the mechanisms through which E2 participates in anterior pituitary cell renewal during the estrous cycle.
format	article
author	Sandra Zárate Gabriela Jaita Jimena Ferraris Guadalupe Eijo María L Magri Daniel Pisera Adriana Seilicovich
author_facet	Sandra Zárate Gabriela Jaita Jimena Ferraris Guadalupe Eijo María L Magri Daniel Pisera Adriana Seilicovich
author_sort	Sandra Zárate
title	Estrogens induce expression of membrane-associated estrogen receptor α isoforms in lactotropes.
title_short	Estrogens induce expression of membrane-associated estrogen receptor α isoforms in lactotropes.
title_full	Estrogens induce expression of membrane-associated estrogen receptor α isoforms in lactotropes.
title_fullStr	Estrogens induce expression of membrane-associated estrogen receptor α isoforms in lactotropes.
title_full_unstemmed	Estrogens induce expression of membrane-associated estrogen receptor α isoforms in lactotropes.
title_sort	estrogens induce expression of membrane-associated estrogen receptor α isoforms in lactotropes.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/4ac7248c94134090a621c38474511624
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Estrogens induce expression of membrane-associated estrogen receptor α isoforms in lactotropes.

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