Identification and biosynthesis of a novel xanthomonadin-dialkylresorcinol-hybrid from Azoarcus sp. BH72.

A novel xanthomonadin-dialkylresorcinol hybrid named arcuflavin was identified in Azoarcus sp. BH72 by a combination of feeding experiments, HPLC-MS and MALDI-MS and gene clusters encoding the biosynthesis of this non-isoprenoid aryl-polyene containing pigment are reported. A chorismate-utilizing en...

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Autores principales:	Tim A Schöner, Sebastian W Fuchs, Barbara Reinhold-Hurek, Helge B Bode
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/4ad31ca04c564206ac85e276fd1e4e6f
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spelling	oai:doaj.org-article:4ad31ca04c564206ac85e276fd1e4e6f2021-11-18T08:28:46ZIdentification and biosynthesis of a novel xanthomonadin-dialkylresorcinol-hybrid from Azoarcus sp. BH72.1932-620310.1371/journal.pone.0090922https://doaj.org/article/4ad31ca04c564206ac85e276fd1e4e6f2014-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24618669/?tool=EBIhttps://doaj.org/toc/1932-6203A novel xanthomonadin-dialkylresorcinol hybrid named arcuflavin was identified in Azoarcus sp. BH72 by a combination of feeding experiments, HPLC-MS and MALDI-MS and gene clusters encoding the biosynthesis of this non-isoprenoid aryl-polyene containing pigment are reported. A chorismate-utilizing enzyme from the XanB2-type producing 3- and 4-hydroxybenzoic acid and an AMP-ligase encoded by these gene clusters were characterized, that might perform the first two steps of the polyene biosynthesis. Furthermore, a detailed analysis of the already known or novel biosynthesis gene clusters involved in the biosynthesis of polyene containing pigments like arcuflavin, flexirubin and xanthomonadin revealed the presence of similar gene clusters in a wide range of bacterial taxa, suggesting that polyene and polyene-dialkylresorcinol pigments are more widespread than previously realized.Tim A SchönerSebastian W FuchsBarbara Reinhold-HurekHelge B BodePublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 3, p e90922 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Tim A Schöner Sebastian W Fuchs Barbara Reinhold-Hurek Helge B Bode Identification and biosynthesis of a novel xanthomonadin-dialkylresorcinol-hybrid from Azoarcus sp. BH72.
description	A novel xanthomonadin-dialkylresorcinol hybrid named arcuflavin was identified in Azoarcus sp. BH72 by a combination of feeding experiments, HPLC-MS and MALDI-MS and gene clusters encoding the biosynthesis of this non-isoprenoid aryl-polyene containing pigment are reported. A chorismate-utilizing enzyme from the XanB2-type producing 3- and 4-hydroxybenzoic acid and an AMP-ligase encoded by these gene clusters were characterized, that might perform the first two steps of the polyene biosynthesis. Furthermore, a detailed analysis of the already known or novel biosynthesis gene clusters involved in the biosynthesis of polyene containing pigments like arcuflavin, flexirubin and xanthomonadin revealed the presence of similar gene clusters in a wide range of bacterial taxa, suggesting that polyene and polyene-dialkylresorcinol pigments are more widespread than previously realized.
format	article
author	Tim A Schöner Sebastian W Fuchs Barbara Reinhold-Hurek Helge B Bode
author_facet	Tim A Schöner Sebastian W Fuchs Barbara Reinhold-Hurek Helge B Bode
author_sort	Tim A Schöner
title	Identification and biosynthesis of a novel xanthomonadin-dialkylresorcinol-hybrid from Azoarcus sp. BH72.
title_short	Identification and biosynthesis of a novel xanthomonadin-dialkylresorcinol-hybrid from Azoarcus sp. BH72.
title_full	Identification and biosynthesis of a novel xanthomonadin-dialkylresorcinol-hybrid from Azoarcus sp. BH72.
title_fullStr	Identification and biosynthesis of a novel xanthomonadin-dialkylresorcinol-hybrid from Azoarcus sp. BH72.
title_full_unstemmed	Identification and biosynthesis of a novel xanthomonadin-dialkylresorcinol-hybrid from Azoarcus sp. BH72.
title_sort	identification and biosynthesis of a novel xanthomonadin-dialkylresorcinol-hybrid from azoarcus sp. bh72.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/4ad31ca04c564206ac85e276fd1e4e6f
work_keys_str_mv	AT timaschoner identificationandbiosynthesisofanovelxanthomonadindialkylresorcinolhybridfromazoarcusspbh72 AT sebastianwfuchs identificationandbiosynthesisofanovelxanthomonadindialkylresorcinolhybridfromazoarcusspbh72 AT barbarareinholdhurek identificationandbiosynthesisofanovelxanthomonadindialkylresorcinolhybridfromazoarcusspbh72 AT helgebbode identificationandbiosynthesisofanovelxanthomonadindialkylresorcinolhybridfromazoarcusspbh72
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Identification and biosynthesis of a novel xanthomonadin-dialkylresorcinol-hybrid from Azoarcus sp. BH72.

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