Effects of Green Silver Nanoparticles on Apoptosis and Oxidative Stress in Normal and Cancerous Human Hepatic Cells in vitro

May Bin-Jumah,1 Monera AL-Abdan,1 Gadah Albasher,2 Saud Alarifi2 1Department of Biology, College of Science, Princess Nourah Bint Abdulrahman University, Riyadh, Saudi Arabia; 2Department of Zoology, College of Science, King Saud University, Riyadh, Saudi ArabiaCorrespondence: Saud AlarifiDepartment...

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Autores principales:	Bin-Jumah M, AL-Abdan M, Albasher G, Alarifi S
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2020
Materias:	green silver nanoparticles cytotoxicity chang and huh-7 cells genotoxicity apoptosis Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/4ad5b03b47ad45578f2136172fa3919a
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spelling	oai:doaj.org-article:4ad5b03b47ad45578f2136172fa3919a2021-12-02T08:05:14ZEffects of Green Silver Nanoparticles on Apoptosis and Oxidative Stress in Normal and Cancerous Human Hepatic Cells in vitro1178-2013https://doaj.org/article/4ad5b03b47ad45578f2136172fa3919a2020-03-01T00:00:00Zhttps://www.dovepress.com/effects-of-green-silver-nanoparticles-on-apoptosis-and-oxidative-stres-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013May Bin-Jumah,1 Monera AL-Abdan,1 Gadah Albasher,2 Saud Alarifi2 1Department of Biology, College of Science, Princess Nourah Bint Abdulrahman University, Riyadh, Saudi Arabia; 2Department of Zoology, College of Science, King Saud University, Riyadh, Saudi ArabiaCorrespondence: Saud AlarifiDepartment of Zoology, College of Science, King Saud University, BOX 2455, Riyadh 11451, Saudi ArabiaTel +966-14679816Fax +966 4678514Email salarifi@ksu.edu.saIntroduction: Extensive use of metallic nanomaterials in different areas of agriculture and commercial products induce significant harmful effects on human health and the environment. In the current study, we synthesized an eco-friendly approach silver nanoparticles (AgNPs) using root extracts of Beta vulgaris L.Methods: The synthesized green silver nanoparticles (gAgNPs) were characterized by dynamic light scattering (DLS) and high-resolution transmission electron microscope (HR-TEM). The gAgNPs had a round shape and the mean size was 20− 50 nm. The cytotoxic effects of gAgNPs were determined in human hepatic normal (CHANG) and cancer (HUH-7) cells by using tetrazolium salt (MTT) and lactate dehydrogenase (LDH) assays for 24 h.Results and Discussion: It was clear from the observations of this experiment that higher concentrations of gAgNPs reduce cell viability. The production of reactive oxygen species (ROS) was evaluated by using DCFDA. The gAgNPs induced more ROS in the HuH-7 cells than in the CHANG cells. The fragmentation of DNA was evaluated by alkaline single-cell gel electrophoresis and the maximum DNA strand breakage was found at a higher concentration exposure of gAgNPs for 24 h. It is important to notice that the HuH-7 cells showed an increased sensitivity to gAgNPs than the CHANG cells. The apoptotic and necrotic effects of gAgNPs on both the cells were evaluated using annexin-V-FITC and propidium iodide staining. An increased count of apoptotic and necrotic cells was found following a higher concentration exposure of gAgNPs. The apoptotic protein expression in these cells due to gAgNPs exposure was determined using immunoblotting techniques and the level of Bcl2 was decreased. However, the expression of BAX and protein was increased in both cells.Conclusion: Therefore, it can be concluded that higher concentrations of gAgNPs may induce significant cytotoxicity and cause DNA damage and apoptosis.Keywords: green silver nanoparticles, cytotoxicity, CHANG and HuH-7 cells, genotoxicity, apoptosisBin-Jumah MAL-Abdan MAlbasher GAlarifi SDove Medical Pressarticlegreen silver nanoparticlescytotoxicitychang and huh-7 cellsgenotoxicityapoptosisMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 15, Pp 1537-1548 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	green silver nanoparticles cytotoxicity chang and huh-7 cells genotoxicity apoptosis Medicine (General) R5-920
spellingShingle	green silver nanoparticles cytotoxicity chang and huh-7 cells genotoxicity apoptosis Medicine (General) R5-920 Bin-Jumah M AL-Abdan M Albasher G Alarifi S Effects of Green Silver Nanoparticles on Apoptosis and Oxidative Stress in Normal and Cancerous Human Hepatic Cells in vitro
description	May Bin-Jumah,1 Monera AL-Abdan,1 Gadah Albasher,2 Saud Alarifi2 1Department of Biology, College of Science, Princess Nourah Bint Abdulrahman University, Riyadh, Saudi Arabia; 2Department of Zoology, College of Science, King Saud University, Riyadh, Saudi ArabiaCorrespondence: Saud AlarifiDepartment of Zoology, College of Science, King Saud University, BOX 2455, Riyadh 11451, Saudi ArabiaTel +966-14679816Fax +966 4678514Email salarifi@ksu.edu.saIntroduction: Extensive use of metallic nanomaterials in different areas of agriculture and commercial products induce significant harmful effects on human health and the environment. In the current study, we synthesized an eco-friendly approach silver nanoparticles (AgNPs) using root extracts of Beta vulgaris L.Methods: The synthesized green silver nanoparticles (gAgNPs) were characterized by dynamic light scattering (DLS) and high-resolution transmission electron microscope (HR-TEM). The gAgNPs had a round shape and the mean size was 20− 50 nm. The cytotoxic effects of gAgNPs were determined in human hepatic normal (CHANG) and cancer (HUH-7) cells by using tetrazolium salt (MTT) and lactate dehydrogenase (LDH) assays for 24 h.Results and Discussion: It was clear from the observations of this experiment that higher concentrations of gAgNPs reduce cell viability. The production of reactive oxygen species (ROS) was evaluated by using DCFDA. The gAgNPs induced more ROS in the HuH-7 cells than in the CHANG cells. The fragmentation of DNA was evaluated by alkaline single-cell gel electrophoresis and the maximum DNA strand breakage was found at a higher concentration exposure of gAgNPs for 24 h. It is important to notice that the HuH-7 cells showed an increased sensitivity to gAgNPs than the CHANG cells. The apoptotic and necrotic effects of gAgNPs on both the cells were evaluated using annexin-V-FITC and propidium iodide staining. An increased count of apoptotic and necrotic cells was found following a higher concentration exposure of gAgNPs. The apoptotic protein expression in these cells due to gAgNPs exposure was determined using immunoblotting techniques and the level of Bcl2 was decreased. However, the expression of BAX and protein was increased in both cells.Conclusion: Therefore, it can be concluded that higher concentrations of gAgNPs may induce significant cytotoxicity and cause DNA damage and apoptosis.Keywords: green silver nanoparticles, cytotoxicity, CHANG and HuH-7 cells, genotoxicity, apoptosis
format	article
author	Bin-Jumah M AL-Abdan M Albasher G Alarifi S
author_facet	Bin-Jumah M AL-Abdan M Albasher G Alarifi S
author_sort	Bin-Jumah M
title	Effects of Green Silver Nanoparticles on Apoptosis and Oxidative Stress in Normal and Cancerous Human Hepatic Cells in vitro
title_short	Effects of Green Silver Nanoparticles on Apoptosis and Oxidative Stress in Normal and Cancerous Human Hepatic Cells in vitro
title_full	Effects of Green Silver Nanoparticles on Apoptosis and Oxidative Stress in Normal and Cancerous Human Hepatic Cells in vitro
title_fullStr	Effects of Green Silver Nanoparticles on Apoptosis and Oxidative Stress in Normal and Cancerous Human Hepatic Cells in vitro
title_full_unstemmed	Effects of Green Silver Nanoparticles on Apoptosis and Oxidative Stress in Normal and Cancerous Human Hepatic Cells in vitro
title_sort	effects of green silver nanoparticles on apoptosis and oxidative stress in normal and cancerous human hepatic cells in vitro
publisher	Dove Medical Press
publishDate	2020
url	https://doaj.org/article/4ad5b03b47ad45578f2136172fa3919a
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Effects of Green Silver Nanoparticles on Apoptosis and Oxidative Stress in Normal and Cancerous Human Hepatic Cells in vitro

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