Concurrent Host-Pathogen Transcriptional Responses in a <italic toggle="yes">Clostridium perfringens</italic> Murine Myonecrosis Infection

ABSTRACT To obtain an insight into host-pathogen interactions in clostridial myonecrosis, we carried out comparative transcriptome analysis of both the bacterium and the host in a murine Clostridium perfringens infection model, which is the first time that such an investigation has been conducted. A...

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Autores principales:	Lee-Yean Low, Paul F. Harrison, Jodee Gould, David R. Powell, Jocelyn M. Choo, Samuel C. Forster, Ross Chapman, Linden J. Gearing, Jackie K. Cheung, Paul Hertzog, Julian I. Rood
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2018
Materias:	clostridial myonecrosis Clostridium perfringens RNA-seq gas gangrene host-pathogen interactions inflammasome Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/4ad7616006074ac0b0e8ec60d59ddcd5
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spelling	oai:doaj.org-article:4ad7616006074ac0b0e8ec60d59ddcd52021-11-15T15:53:26ZConcurrent Host-Pathogen Transcriptional Responses in a <italic toggle="yes">Clostridium perfringens</italic> Murine Myonecrosis Infection10.1128/mBio.00473-182150-7511https://doaj.org/article/4ad7616006074ac0b0e8ec60d59ddcd52018-05-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/mBio.00473-18https://doaj.org/toc/2150-7511ABSTRACT To obtain an insight into host-pathogen interactions in clostridial myonecrosis, we carried out comparative transcriptome analysis of both the bacterium and the host in a murine Clostridium perfringens infection model, which is the first time that such an investigation has been conducted. Analysis of the host transcriptome from infected muscle tissues indicated that many genes were upregulated compared to the results seen with mock-infected mice. These genes were enriched for host defense pathways, including Toll-like receptor (TLR) and Nod-like receptor (NLR) signaling components. Real-time PCR confirmed that host TLR2 and NLRP3 inflammasome genes were induced in response to C. perfringens infection. Comparison of the transcriptome of C. perfringens cells from the infected tissues with that from broth cultures showed that host selective pressure induced a global change in C. perfringens gene expression. A total of 33% (923) of C. perfringens genes were differentially regulated, including 10 potential virulence genes that were upregulated relative to their expression in vitro. These genes encoded putative proteins that may be involved in the synthesis of cell wall-associated macromolecules, in adhesion to host cells, or in protection from host cationic antimicrobial peptides. This report presents the first successful expression profiling of coregulated transcriptomes of bacterial and host genes during a clostridial myonecrosis infection and provides new insights into disease pathogenesis and host-pathogen interactions. IMPORTANCE Clostridium perfringens is the causative agent of traumatic clostridial myonecrosis, or gas gangrene. In this study, we carried out transcriptional analysis of both the host and the bacterial pathogen in a mouse myonecrosis infection. The results showed that in comparison to mock-infected control tissues, muscle tissues from C. perfringens-infected mice had a significantly altered gene expression profile. In particular, the expression of many genes involved in the innate immune system was upregulated. Comparison of the expression profiles of C. perfringens cells isolated from the infected tissues with those from equivalent broth cultures identified many potential virulence genes that were significantly upregulated in vivo. These studies have provided a new understanding of the range of factors involved in host-pathogen interactions in a myonecrosis infection.Lee-Yean LowPaul F. HarrisonJodee GouldDavid R. PowellJocelyn M. ChooSamuel C. ForsterRoss ChapmanLinden J. GearingJackie K. CheungPaul HertzogJulian I. RoodAmerican Society for Microbiologyarticleclostridial myonecrosisClostridium perfringensRNA-seqgas gangrenehost-pathogen interactionsinflammasomeMicrobiologyQR1-502ENmBio, Vol 9, Iss 2 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	clostridial myonecrosis Clostridium perfringens RNA-seq gas gangrene host-pathogen interactions inflammasome Microbiology QR1-502
spellingShingle	clostridial myonecrosis Clostridium perfringens RNA-seq gas gangrene host-pathogen interactions inflammasome Microbiology QR1-502 Lee-Yean Low Paul F. Harrison Jodee Gould David R. Powell Jocelyn M. Choo Samuel C. Forster Ross Chapman Linden J. Gearing Jackie K. Cheung Paul Hertzog Julian I. Rood Concurrent Host-Pathogen Transcriptional Responses in a <italic toggle="yes">Clostridium perfringens</italic> Murine Myonecrosis Infection
description	ABSTRACT To obtain an insight into host-pathogen interactions in clostridial myonecrosis, we carried out comparative transcriptome analysis of both the bacterium and the host in a murine Clostridium perfringens infection model, which is the first time that such an investigation has been conducted. Analysis of the host transcriptome from infected muscle tissues indicated that many genes were upregulated compared to the results seen with mock-infected mice. These genes were enriched for host defense pathways, including Toll-like receptor (TLR) and Nod-like receptor (NLR) signaling components. Real-time PCR confirmed that host TLR2 and NLRP3 inflammasome genes were induced in response to C. perfringens infection. Comparison of the transcriptome of C. perfringens cells from the infected tissues with that from broth cultures showed that host selective pressure induced a global change in C. perfringens gene expression. A total of 33% (923) of C. perfringens genes were differentially regulated, including 10 potential virulence genes that were upregulated relative to their expression in vitro. These genes encoded putative proteins that may be involved in the synthesis of cell wall-associated macromolecules, in adhesion to host cells, or in protection from host cationic antimicrobial peptides. This report presents the first successful expression profiling of coregulated transcriptomes of bacterial and host genes during a clostridial myonecrosis infection and provides new insights into disease pathogenesis and host-pathogen interactions. IMPORTANCE Clostridium perfringens is the causative agent of traumatic clostridial myonecrosis, or gas gangrene. In this study, we carried out transcriptional analysis of both the host and the bacterial pathogen in a mouse myonecrosis infection. The results showed that in comparison to mock-infected control tissues, muscle tissues from C. perfringens-infected mice had a significantly altered gene expression profile. In particular, the expression of many genes involved in the innate immune system was upregulated. Comparison of the expression profiles of C. perfringens cells isolated from the infected tissues with those from equivalent broth cultures identified many potential virulence genes that were significantly upregulated in vivo. These studies have provided a new understanding of the range of factors involved in host-pathogen interactions in a myonecrosis infection.
format	article
author	Lee-Yean Low Paul F. Harrison Jodee Gould David R. Powell Jocelyn M. Choo Samuel C. Forster Ross Chapman Linden J. Gearing Jackie K. Cheung Paul Hertzog Julian I. Rood
author_facet	Lee-Yean Low Paul F. Harrison Jodee Gould David R. Powell Jocelyn M. Choo Samuel C. Forster Ross Chapman Linden J. Gearing Jackie K. Cheung Paul Hertzog Julian I. Rood
author_sort	Lee-Yean Low
title	Concurrent Host-Pathogen Transcriptional Responses in a <italic toggle="yes">Clostridium perfringens</italic> Murine Myonecrosis Infection
title_short	Concurrent Host-Pathogen Transcriptional Responses in a <italic toggle="yes">Clostridium perfringens</italic> Murine Myonecrosis Infection
title_full	Concurrent Host-Pathogen Transcriptional Responses in a <italic toggle="yes">Clostridium perfringens</italic> Murine Myonecrosis Infection
title_fullStr	Concurrent Host-Pathogen Transcriptional Responses in a <italic toggle="yes">Clostridium perfringens</italic> Murine Myonecrosis Infection
title_full_unstemmed	Concurrent Host-Pathogen Transcriptional Responses in a <italic toggle="yes">Clostridium perfringens</italic> Murine Myonecrosis Infection
title_sort	concurrent host-pathogen transcriptional responses in a <italic toggle="yes">clostridium perfringens</italic> murine myonecrosis infection
publisher	American Society for Microbiology
publishDate	2018
url	https://doaj.org/article/4ad7616006074ac0b0e8ec60d59ddcd5
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Concurrent Host-Pathogen Transcriptional Responses in a <italic toggle="yes">Clostridium perfringens</italic> Murine Myonecrosis Infection

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