Adverse effects from clenbuterol and ractopamine on nematode Caenorhabditis elegans and the underlying mechanism.

In the present study, we used Caenorhabditis elegans assay system to investigate in vivo toxicity from clentuberol and ractopamine and the possible underlying mechanism. Both acute and prolonged exposures to clentuberol or ractopamine decreased brood size and locomotion behavior, and induced intesti...

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Autores principales:	Ziheng Zhuang, Yunli Zhao, Qiuli Wu, Min Li, Haicui Liu, Lingmei Sun, Wei Gao, Dayong Wang
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/4adbc99e3dbc41feaae858c8f7d31603
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spelling	oai:doaj.org-article:4adbc99e3dbc41feaae858c8f7d316032021-11-18T08:37:02ZAdverse effects from clenbuterol and ractopamine on nematode Caenorhabditis elegans and the underlying mechanism.1932-620310.1371/journal.pone.0085482https://doaj.org/article/4adbc99e3dbc41feaae858c8f7d316032014-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24465573/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203In the present study, we used Caenorhabditis elegans assay system to investigate in vivo toxicity from clentuberol and ractopamine and the possible underlying mechanism. Both acute and prolonged exposures to clentuberol or ractopamine decreased brood size and locomotion behavior, and induced intestinal autofluorescence and reactive oxygen species (ROS) production. Although acute exposure to the examined concentrations of clentuberol or ractopamine did not induce lethality, prolonged exposure to 10 µg/L of clentuberol and ractopamine reduced lifespan. At relatively high concentrations, ractopamine exhibited more severe toxicity than clentuberol on nematodes. Overexpression of sod-2 gene encoding a Mn-SOD to prevent induction of oxidative stress effectively inhibited toxicity from clentuberol or ractopamine. Besides oxidative stress, we found that clentuberol might reduce lifespan through influencing insulin/IGF signaling pathway; however, ractopamine might reduce lifespan through affecting both insulin/IGF signaling pathway and TOR signaling pathway. Ractopamine more severely decreased expression levels of daf-16, sgk-1, skn-1, and aak-2 genes than clentuberol, and increased expression levels of daf-2 and age-1 genes at the examined concentration. Therefore, the C. elegans assay system may be useful for assessing the possible toxicity from weight loss agents, and clentuberol and ractopamine may induce toxicity through different molecular mechanisms.Ziheng ZhuangYunli ZhaoQiuli WuMin LiHaicui LiuLingmei SunWei GaoDayong WangPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 1, p e85482 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Ziheng Zhuang Yunli Zhao Qiuli Wu Min Li Haicui Liu Lingmei Sun Wei Gao Dayong Wang Adverse effects from clenbuterol and ractopamine on nematode Caenorhabditis elegans and the underlying mechanism.
description	In the present study, we used Caenorhabditis elegans assay system to investigate in vivo toxicity from clentuberol and ractopamine and the possible underlying mechanism. Both acute and prolonged exposures to clentuberol or ractopamine decreased brood size and locomotion behavior, and induced intestinal autofluorescence and reactive oxygen species (ROS) production. Although acute exposure to the examined concentrations of clentuberol or ractopamine did not induce lethality, prolonged exposure to 10 µg/L of clentuberol and ractopamine reduced lifespan. At relatively high concentrations, ractopamine exhibited more severe toxicity than clentuberol on nematodes. Overexpression of sod-2 gene encoding a Mn-SOD to prevent induction of oxidative stress effectively inhibited toxicity from clentuberol or ractopamine. Besides oxidative stress, we found that clentuberol might reduce lifespan through influencing insulin/IGF signaling pathway; however, ractopamine might reduce lifespan through affecting both insulin/IGF signaling pathway and TOR signaling pathway. Ractopamine more severely decreased expression levels of daf-16, sgk-1, skn-1, and aak-2 genes than clentuberol, and increased expression levels of daf-2 and age-1 genes at the examined concentration. Therefore, the C. elegans assay system may be useful for assessing the possible toxicity from weight loss agents, and clentuberol and ractopamine may induce toxicity through different molecular mechanisms.
format	article
author	Ziheng Zhuang Yunli Zhao Qiuli Wu Min Li Haicui Liu Lingmei Sun Wei Gao Dayong Wang
author_facet	Ziheng Zhuang Yunli Zhao Qiuli Wu Min Li Haicui Liu Lingmei Sun Wei Gao Dayong Wang
author_sort	Ziheng Zhuang
title	Adverse effects from clenbuterol and ractopamine on nematode Caenorhabditis elegans and the underlying mechanism.
title_short	Adverse effects from clenbuterol and ractopamine on nematode Caenorhabditis elegans and the underlying mechanism.
title_full	Adverse effects from clenbuterol and ractopamine on nematode Caenorhabditis elegans and the underlying mechanism.
title_fullStr	Adverse effects from clenbuterol and ractopamine on nematode Caenorhabditis elegans and the underlying mechanism.
title_full_unstemmed	Adverse effects from clenbuterol and ractopamine on nematode Caenorhabditis elegans and the underlying mechanism.
title_sort	adverse effects from clenbuterol and ractopamine on nematode caenorhabditis elegans and the underlying mechanism.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/4adbc99e3dbc41feaae858c8f7d31603
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Adverse effects from clenbuterol and ractopamine on nematode Caenorhabditis elegans and the underlying mechanism.

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