Ruminant fat intake improves gut microbiota, serum inflammatory parameter and fatty acid profile in tissues of Wistar rats

Abstract This study tested the hypothesis that naturally and industrially produced trans-fatty acids can exert distinct effects on metabolic parameters and on gut microbiota of rats. Wistar rats were randomized into three groups according to the diet: CONT-control, with 5% soybean oil and normal amo...

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Autores principales:	Larissa de Brito Medeiros, Susana Paula Almeida Alves, Rui José Branquinho de Bessa, Juliana Késsia Barbosa Soares, Camila Neves Meireles Costa, Jailane de Souza Aquino, Gerlane Coelho Bernardo Guerra, Daline Fernandes de Souza Araújo, Lydiane Tavares Toscano, Alexandre Sérgio Silva, Adriano Francisco Alves, Mateus Lacerda Pereira Lemos, Wydemberg José de Araujo, Ariosvaldo Nunes de Medeiros, Celso José Bruno de Oliveira, Rita de Cassia Ramos do Egypto Queiroga
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/4adc391287b8433ea80f89c795bcfda8
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spelling	oai:doaj.org-article:4adc391287b8433ea80f89c795bcfda82021-12-02T18:48:23ZRuminant fat intake improves gut microbiota, serum inflammatory parameter and fatty acid profile in tissues of Wistar rats10.1038/s41598-021-98248-62045-2322https://doaj.org/article/4adc391287b8433ea80f89c795bcfda82021-09-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-98248-6https://doaj.org/toc/2045-2322Abstract This study tested the hypothesis that naturally and industrially produced trans-fatty acids can exert distinct effects on metabolic parameters and on gut microbiota of rats. Wistar rats were randomized into three groups according to the diet: CONT-control, with 5% soybean oil and normal amount of fat; HVF-20% of hydrogenated vegetable fat (industrial); and RUM-20% of ruminant fat (natural). After 53 days of treatment, serum biochemical markers, fatty acid composition of liver, heart and adipose tissue, histology and hepatic oxidative parameters, as well as gut microbiota composition were evaluated. HVF diet intake reduced triglycerides (≈ 39.39%) and VLDL levels (≈ 39.49%). Trans-fatty acids levels in all tissue were higher in HVF group. However, RUM diet intake elevated amounts of anti-inflammatory cytokine IL-10 (≈ 14.7%) compared to CONT, but not to HVF. Furthermore, RUM intake led to higher concentrations of stearic acid and conjugated linoleic acid in all tissue; this particular diet was associated with a hepatoprotective effect. The microbial gut communities were significantly different among the groups. Our results show that ruminant fat reversed the hepatic steatosis normally caused by high fat diets, which may be related to the remodelling of the gut microbiota and its anti-inflammatory potential.Larissa de Brito MedeirosSusana Paula Almeida AlvesRui José Branquinho de BessaJuliana Késsia Barbosa SoaresCamila Neves Meireles CostaJailane de Souza AquinoGerlane Coelho Bernardo GuerraDaline Fernandes de Souza AraújoLydiane Tavares ToscanoAlexandre Sérgio SilvaAdriano Francisco AlvesMateus Lacerda Pereira LemosWydemberg José de AraujoAriosvaldo Nunes de MedeirosCelso José Bruno de OliveiraRita de Cassia Ramos do Egypto QueirogaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-17 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Larissa de Brito Medeiros Susana Paula Almeida Alves Rui José Branquinho de Bessa Juliana Késsia Barbosa Soares Camila Neves Meireles Costa Jailane de Souza Aquino Gerlane Coelho Bernardo Guerra Daline Fernandes de Souza Araújo Lydiane Tavares Toscano Alexandre Sérgio Silva Adriano Francisco Alves Mateus Lacerda Pereira Lemos Wydemberg José de Araujo Ariosvaldo Nunes de Medeiros Celso José Bruno de Oliveira Rita de Cassia Ramos do Egypto Queiroga Ruminant fat intake improves gut microbiota, serum inflammatory parameter and fatty acid profile in tissues of Wistar rats
description	Abstract This study tested the hypothesis that naturally and industrially produced trans-fatty acids can exert distinct effects on metabolic parameters and on gut microbiota of rats. Wistar rats were randomized into three groups according to the diet: CONT-control, with 5% soybean oil and normal amount of fat; HVF-20% of hydrogenated vegetable fat (industrial); and RUM-20% of ruminant fat (natural). After 53 days of treatment, serum biochemical markers, fatty acid composition of liver, heart and adipose tissue, histology and hepatic oxidative parameters, as well as gut microbiota composition were evaluated. HVF diet intake reduced triglycerides (≈ 39.39%) and VLDL levels (≈ 39.49%). Trans-fatty acids levels in all tissue were higher in HVF group. However, RUM diet intake elevated amounts of anti-inflammatory cytokine IL-10 (≈ 14.7%) compared to CONT, but not to HVF. Furthermore, RUM intake led to higher concentrations of stearic acid and conjugated linoleic acid in all tissue; this particular diet was associated with a hepatoprotective effect. The microbial gut communities were significantly different among the groups. Our results show that ruminant fat reversed the hepatic steatosis normally caused by high fat diets, which may be related to the remodelling of the gut microbiota and its anti-inflammatory potential.
format	article
author	Larissa de Brito Medeiros Susana Paula Almeida Alves Rui José Branquinho de Bessa Juliana Késsia Barbosa Soares Camila Neves Meireles Costa Jailane de Souza Aquino Gerlane Coelho Bernardo Guerra Daline Fernandes de Souza Araújo Lydiane Tavares Toscano Alexandre Sérgio Silva Adriano Francisco Alves Mateus Lacerda Pereira Lemos Wydemberg José de Araujo Ariosvaldo Nunes de Medeiros Celso José Bruno de Oliveira Rita de Cassia Ramos do Egypto Queiroga
author_facet	Larissa de Brito Medeiros Susana Paula Almeida Alves Rui José Branquinho de Bessa Juliana Késsia Barbosa Soares Camila Neves Meireles Costa Jailane de Souza Aquino Gerlane Coelho Bernardo Guerra Daline Fernandes de Souza Araújo Lydiane Tavares Toscano Alexandre Sérgio Silva Adriano Francisco Alves Mateus Lacerda Pereira Lemos Wydemberg José de Araujo Ariosvaldo Nunes de Medeiros Celso José Bruno de Oliveira Rita de Cassia Ramos do Egypto Queiroga
author_sort	Larissa de Brito Medeiros
title	Ruminant fat intake improves gut microbiota, serum inflammatory parameter and fatty acid profile in tissues of Wistar rats
title_short	Ruminant fat intake improves gut microbiota, serum inflammatory parameter and fatty acid profile in tissues of Wistar rats
title_full	Ruminant fat intake improves gut microbiota, serum inflammatory parameter and fatty acid profile in tissues of Wistar rats
title_fullStr	Ruminant fat intake improves gut microbiota, serum inflammatory parameter and fatty acid profile in tissues of Wistar rats
title_full_unstemmed	Ruminant fat intake improves gut microbiota, serum inflammatory parameter and fatty acid profile in tissues of Wistar rats
title_sort	ruminant fat intake improves gut microbiota, serum inflammatory parameter and fatty acid profile in tissues of wistar rats
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/4adc391287b8433ea80f89c795bcfda8
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Ruminant fat intake improves gut microbiota, serum inflammatory parameter and fatty acid profile in tissues of Wistar rats

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