Cryopreservation of Indonesian native chicken semen by using dimethyl sulfoxide and various level of ethylene glycol as cryoprotectants

Abstract. Khaeruddin, Junaedi, Hastuti. 2020. Cryopreservation of Indonesian native chicken semen by using dimethyl sulfoxide and various level of ethylene glycol as cryoprotectants. Biodiversitas 21: 5718-5722. Imported purebred chickens are becoming more popular and a regular staple in Indonesia....

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Autores principales:	KHAERUDDIN KHAERUDDIN, JUNAEDI JUNAEDI, HASTUTI HASTUTI
Formato:	article
Lenguaje:	EN
Publicado:	MBI & UNS Solo 2020
Materias:	cryopreservation, dmso, ethylene glycol, semen chicken Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/4addaea10d7c4026965bdcf5eb4fb383
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spelling	oai:doaj.org-article:4addaea10d7c4026965bdcf5eb4fb3832021-11-22T00:52:01ZCryopreservation of Indonesian native chicken semen by using dimethyl sulfoxide and various level of ethylene glycol as cryoprotectants1412-033X2085-472210.13057/biodiv/d211231https://doaj.org/article/4addaea10d7c4026965bdcf5eb4fb3832020-11-01T00:00:00Zhttps://smujo.id/biodiv/article/view/6830https://doaj.org/toc/1412-033Xhttps://doaj.org/toc/2085-4722Abstract. Khaeruddin, Junaedi, Hastuti. 2020. Cryopreservation of Indonesian native chicken semen by using dimethyl sulfoxide and various level of ethylene glycol as cryoprotectants. Biodiversitas 21: 5718-5722. Imported purebred chickens are becoming more popular and a regular staple in Indonesia. Therefore, it is necessary to strengthen conservation efforts to preserve Indonesian chickens, one of which is by means of sperm cryopreservation. This study aimed to determine the effects of the addition of DMSO and different concentrations of ethylene glycol to a Ringer’s lactate egg yolk (RLY)-or coconut water egg yolk (CWY)-based extender on the quality of frozen-thawed Indonesian chicken sperm. This study was used nine Indonesian native roosters about 20 months of age. The semen extenders used in this study were RLY + DMSO 7%, RLY + ethylene glycol 3%, RLY + ethylene glycol 5%, RLY + ethylene glycol 7%, CWY + DMSO 7%, CWY + ethylene glycol 3%, CWY + ethylene glycol 5% and CWY + ethylene glycol 7%. Liquid semen was packaged in 0.25 mL straw, then cooled at 5oC for 2 hours, frozen at 5 cm above liquid nitrogen for 10 minutes, following stored in a liquid nitrogen container for 24 hours. The semen straws were thawed at 37oC for 30 seconds. Statistical analysis for multiple comparisons was performed as a completely randomized design with eight treatment levels and seven replications. The results showed that there were no differences in sperm motility, recovery rate, and abnormality between extenders after the freeze-thaw process. Whereas, RLY + DMSO 7% was the highest sperm viability.KHAERUDDIN KHAERUDDINJUNAEDI JUNAEDIHASTUTI HASTUTIMBI & UNS Soloarticlecryopreservation, dmso, ethylene glycol, semen chickenBiology (General)QH301-705.5ENBiodiversitas, Vol 21, Iss 12 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	cryopreservation, dmso, ethylene glycol, semen chicken Biology (General) QH301-705.5
spellingShingle	cryopreservation, dmso, ethylene glycol, semen chicken Biology (General) QH301-705.5 KHAERUDDIN KHAERUDDIN JUNAEDI JUNAEDI HASTUTI HASTUTI Cryopreservation of Indonesian native chicken semen by using dimethyl sulfoxide and various level of ethylene glycol as cryoprotectants
description	Abstract. Khaeruddin, Junaedi, Hastuti. 2020. Cryopreservation of Indonesian native chicken semen by using dimethyl sulfoxide and various level of ethylene glycol as cryoprotectants. Biodiversitas 21: 5718-5722. Imported purebred chickens are becoming more popular and a regular staple in Indonesia. Therefore, it is necessary to strengthen conservation efforts to preserve Indonesian chickens, one of which is by means of sperm cryopreservation. This study aimed to determine the effects of the addition of DMSO and different concentrations of ethylene glycol to a Ringer’s lactate egg yolk (RLY)-or coconut water egg yolk (CWY)-based extender on the quality of frozen-thawed Indonesian chicken sperm. This study was used nine Indonesian native roosters about 20 months of age. The semen extenders used in this study were RLY + DMSO 7%, RLY + ethylene glycol 3%, RLY + ethylene glycol 5%, RLY + ethylene glycol 7%, CWY + DMSO 7%, CWY + ethylene glycol 3%, CWY + ethylene glycol 5% and CWY + ethylene glycol 7%. Liquid semen was packaged in 0.25 mL straw, then cooled at 5oC for 2 hours, frozen at 5 cm above liquid nitrogen for 10 minutes, following stored in a liquid nitrogen container for 24 hours. The semen straws were thawed at 37oC for 30 seconds. Statistical analysis for multiple comparisons was performed as a completely randomized design with eight treatment levels and seven replications. The results showed that there were no differences in sperm motility, recovery rate, and abnormality between extenders after the freeze-thaw process. Whereas, RLY + DMSO 7% was the highest sperm viability.
format	article
author	KHAERUDDIN KHAERUDDIN JUNAEDI JUNAEDI HASTUTI HASTUTI
author_facet	KHAERUDDIN KHAERUDDIN JUNAEDI JUNAEDI HASTUTI HASTUTI
author_sort	KHAERUDDIN KHAERUDDIN
title	Cryopreservation of Indonesian native chicken semen by using dimethyl sulfoxide and various level of ethylene glycol as cryoprotectants
title_short	Cryopreservation of Indonesian native chicken semen by using dimethyl sulfoxide and various level of ethylene glycol as cryoprotectants
title_full	Cryopreservation of Indonesian native chicken semen by using dimethyl sulfoxide and various level of ethylene glycol as cryoprotectants
title_fullStr	Cryopreservation of Indonesian native chicken semen by using dimethyl sulfoxide and various level of ethylene glycol as cryoprotectants
title_full_unstemmed	Cryopreservation of Indonesian native chicken semen by using dimethyl sulfoxide and various level of ethylene glycol as cryoprotectants
title_sort	cryopreservation of indonesian native chicken semen by using dimethyl sulfoxide and various level of ethylene glycol as cryoprotectants
publisher	MBI & UNS Solo
publishDate	2020
url	https://doaj.org/article/4addaea10d7c4026965bdcf5eb4fb383
work_keys_str_mv	AT khaeruddinkhaeruddin cryopreservationofindonesiannativechickensemenbyusingdimethylsulfoxideandvariouslevelofethyleneglycolascryoprotectants AT junaedijunaedi cryopreservationofindonesiannativechickensemenbyusingdimethylsulfoxideandvariouslevelofethyleneglycolascryoprotectants AT hastutihastuti cryopreservationofindonesiannativechickensemenbyusingdimethylsulfoxideandvariouslevelofethyleneglycolascryoprotectants
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Cryopreservation of Indonesian native chicken semen by using dimethyl sulfoxide and various level of ethylene glycol as cryoprotectants

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