Determination of Glucose with Cellulose Acetate/Glucose Oxidase Modified Carbon Paste Electrodes

An amperometric biosensor for glucose determination was developed using a carbon paste electrode (CPE) modified with cellulose acetate (CA)/glucose oxidase (GOx) bilayer membrane through the electrostatic interaction between them. The CA membrane was used as matrix for enzyme immobilization via micr...

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Autores principales:	Rochmad Kris Sanjaya, Datin An Nisa Sukmawati, Nurul Ismillayli, Dhony Hermanto
Formato:	article
Lenguaje:	EN ID
Publicado:	Jenderal Soedirman University 2021
Materias:	Chemistry QD1-999
Acceso en línea:	https://doaj.org/article/4ae056386991483b8ef3ef2a613d2580
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spelling	oai:doaj.org-article:4ae056386991483b8ef3ef2a613d25802021-11-22T01:13:54ZDetermination of Glucose with Cellulose Acetate/Glucose Oxidase Modified Carbon Paste Electrodes1907-97612503-031010.20884/1.jm.2021.16.3.679https://doaj.org/article/4ae056386991483b8ef3ef2a613d25802021-11-01T00:00:00Zhttps://ojs.jmolekul.com/ojs/index.php/jm/article/view/679https://doaj.org/toc/1907-9761https://doaj.org/toc/2503-0310An amperometric biosensor for glucose determination was developed using a carbon paste electrode (CPE) modified with cellulose acetate (CA)/glucose oxidase (GOx) bilayer membrane through the electrostatic interaction between them. The CA membrane was used as matrix for enzyme immobilization via microencapsulation technique, is enzyme placed between two membranes. CA/GOx membrane was attached to CPE surface containing ferrocene (Fc). By using proposed amperometric biosensor, glucose concentration was determined as well as its characteristic. The modified Fc–graphite electrode with CA/GOx bilayer membrane for glucose had optimum measurement conditions at work emf of 874 mV, CA concentration of 10% and amount Fc of 0.021 mg. The biosensor showed good performance at glucose concentration range of 0.05–3 mM and limit of detection was 0.024 mM. Proposed biosensor has good reproducibility with relative standard deviation (RSD) was less than 5% up to 7 times use (in the defined condition 4 ˚C). Glucose measurement result in human serum of diabetes mellitus patients showed conformity with result of reference method, MediSense Optium glucose test kit.Rochmad Kris SanjayaDatin An Nisa SukmawatiNurul IsmillayliDhony HermantoJenderal Soedirman UniversityarticleChemistryQD1-999ENIDMolekul, Vol 16, Iss 3, Pp 176-183 (2021)
institution	DOAJ
collection	DOAJ
language	EN ID
topic	Chemistry QD1-999
spellingShingle	Chemistry QD1-999 Rochmad Kris Sanjaya Datin An Nisa Sukmawati Nurul Ismillayli Dhony Hermanto Determination of Glucose with Cellulose Acetate/Glucose Oxidase Modified Carbon Paste Electrodes
description	An amperometric biosensor for glucose determination was developed using a carbon paste electrode (CPE) modified with cellulose acetate (CA)/glucose oxidase (GOx) bilayer membrane through the electrostatic interaction between them. The CA membrane was used as matrix for enzyme immobilization via microencapsulation technique, is enzyme placed between two membranes. CA/GOx membrane was attached to CPE surface containing ferrocene (Fc). By using proposed amperometric biosensor, glucose concentration was determined as well as its characteristic. The modified Fc–graphite electrode with CA/GOx bilayer membrane for glucose had optimum measurement conditions at work emf of 874 mV, CA concentration of 10% and amount Fc of 0.021 mg. The biosensor showed good performance at glucose concentration range of 0.05–3 mM and limit of detection was 0.024 mM. Proposed biosensor has good reproducibility with relative standard deviation (RSD) was less than 5% up to 7 times use (in the defined condition 4 ˚C). Glucose measurement result in human serum of diabetes mellitus patients showed conformity with result of reference method, MediSense Optium glucose test kit.
format	article
author	Rochmad Kris Sanjaya Datin An Nisa Sukmawati Nurul Ismillayli Dhony Hermanto
author_facet	Rochmad Kris Sanjaya Datin An Nisa Sukmawati Nurul Ismillayli Dhony Hermanto
author_sort	Rochmad Kris Sanjaya
title	Determination of Glucose with Cellulose Acetate/Glucose Oxidase Modified Carbon Paste Electrodes
title_short	Determination of Glucose with Cellulose Acetate/Glucose Oxidase Modified Carbon Paste Electrodes
title_full	Determination of Glucose with Cellulose Acetate/Glucose Oxidase Modified Carbon Paste Electrodes
title_fullStr	Determination of Glucose with Cellulose Acetate/Glucose Oxidase Modified Carbon Paste Electrodes
title_full_unstemmed	Determination of Glucose with Cellulose Acetate/Glucose Oxidase Modified Carbon Paste Electrodes
title_sort	determination of glucose with cellulose acetate/glucose oxidase modified carbon paste electrodes
publisher	Jenderal Soedirman University
publishDate	2021
url	https://doaj.org/article/4ae056386991483b8ef3ef2a613d2580
work_keys_str_mv	AT rochmadkrissanjaya determinationofglucosewithcelluloseacetateglucoseoxidasemodifiedcarbonpasteelectrodes AT datinannisasukmawati determinationofglucosewithcelluloseacetateglucoseoxidasemodifiedcarbonpasteelectrodes AT nurulismillayli determinationofglucosewithcelluloseacetateglucoseoxidasemodifiedcarbonpasteelectrodes AT dhonyhermanto determinationofglucosewithcelluloseacetateglucoseoxidasemodifiedcarbonpasteelectrodes
_version_	1718418280083881984

Determination of Glucose with Cellulose Acetate/Glucose Oxidase Modified Carbon Paste Electrodes

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