Co-factor-free aggregation of tau into seeding-competent RNA-sequestering amyloid fibrils

The authors present a method for the conversion of full-length tau protein into seeding-competent amyloid fibrils without heparin or other negatively charged co-factors, which could be useful for studying the effects of post-translational modifications on Tau aggregation as well as to identify poten...

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Autores principales:	Pijush Chakraborty, Gwladys Rivière, Shu Liu, Alain Ibáñez de Opakua, Rıza Dervişoğlu, Alina Hebestreit, Loren B. Andreas, Ina M. Vorberg, Markus Zweckstetter
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/4ae08ea82f3e4f1da86a83d9dfe205fb
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

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spelling	oai:doaj.org-article:4ae08ea82f3e4f1da86a83d9dfe205fb2021-12-02T15:22:59ZCo-factor-free aggregation of tau into seeding-competent RNA-sequestering amyloid fibrils10.1038/s41467-021-24362-82041-1723https://doaj.org/article/4ae08ea82f3e4f1da86a83d9dfe205fb2021-07-01T00:00:00Zhttps://doi.org/10.1038/s41467-021-24362-8https://doaj.org/toc/2041-1723The authors present a method for the conversion of full-length tau protein into seeding-competent amyloid fibrils without heparin or other negatively charged co-factors, which could be useful for studying the effects of post-translational modifications on Tau aggregation as well as to identify potential inhibitors of tau aggregation. Biochemical experiments and solid-state NMR spectroscopy measurements show that these co-factor-free tau fibrils have similar properties as amyloid fibrils isolated from brain tissue but differ from those of commonly used heparin-induced tau fibrils.Pijush ChakrabortyGwladys RivièreShu LiuAlain Ibáñez de OpakuaRıza DervişoğluAlina HebestreitLoren B. AndreasIna M. VorbergMarkus ZweckstetterNature PortfolioarticleScienceQENNature Communications, Vol 12, Iss 1, Pp 1-12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Pijush Chakraborty Gwladys Rivière Shu Liu Alain Ibáñez de Opakua Rıza Dervişoğlu Alina Hebestreit Loren B. Andreas Ina M. Vorberg Markus Zweckstetter Co-factor-free aggregation of tau into seeding-competent RNA-sequestering amyloid fibrils
description	The authors present a method for the conversion of full-length tau protein into seeding-competent amyloid fibrils without heparin or other negatively charged co-factors, which could be useful for studying the effects of post-translational modifications on Tau aggregation as well as to identify potential inhibitors of tau aggregation. Biochemical experiments and solid-state NMR spectroscopy measurements show that these co-factor-free tau fibrils have similar properties as amyloid fibrils isolated from brain tissue but differ from those of commonly used heparin-induced tau fibrils.
format	article
author	Pijush Chakraborty Gwladys Rivière Shu Liu Alain Ibáñez de Opakua Rıza Dervişoğlu Alina Hebestreit Loren B. Andreas Ina M. Vorberg Markus Zweckstetter
author_facet	Pijush Chakraborty Gwladys Rivière Shu Liu Alain Ibáñez de Opakua Rıza Dervişoğlu Alina Hebestreit Loren B. Andreas Ina M. Vorberg Markus Zweckstetter
author_sort	Pijush Chakraborty
title	Co-factor-free aggregation of tau into seeding-competent RNA-sequestering amyloid fibrils
title_short	Co-factor-free aggregation of tau into seeding-competent RNA-sequestering amyloid fibrils
title_full	Co-factor-free aggregation of tau into seeding-competent RNA-sequestering amyloid fibrils
title_fullStr	Co-factor-free aggregation of tau into seeding-competent RNA-sequestering amyloid fibrils
title_full_unstemmed	Co-factor-free aggregation of tau into seeding-competent RNA-sequestering amyloid fibrils
title_sort	co-factor-free aggregation of tau into seeding-competent rna-sequestering amyloid fibrils
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/4ae08ea82f3e4f1da86a83d9dfe205fb
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Co-factor-free aggregation of tau into seeding-competent RNA-sequestering amyloid fibrils

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