Virus-derived sequences from the transcriptomes of two snail vectors of schistosomiasis, Biomphalaria pfeifferi and Bulinus globosus from Kenya

Schistosomiasis, which infects more than 230 million people, is vectored by freshwater snails. We identified viral sequences in the transcriptomes of Biomphalaria pfeifferi (BP) and Bulinus globosus (BuG), two of the world’s most important schistosomiasis vectors in Africa. Sequences from 26 snails...

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Autores principales:	Sijun Liu, Si-Ming Zhang, Sarah K. Buddenborg, Eric S. Loker, Bryony C. Bonning
Formato:	article
Lenguaje:	EN
Publicado:	PeerJ Inc. 2021
Materias:	Snail virus Schistosomiasis vector Virus discovery Biomphalaria pfeifferi Bulinus globosus Kenya Medicine R
Acceso en línea:	https://doaj.org/article/4ae13b8ca06642ffbe04d7874fffe3c5
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spelling	oai:doaj.org-article:4ae13b8ca06642ffbe04d7874fffe3c52021-11-17T15:05:08ZVirus-derived sequences from the transcriptomes of two snail vectors of schistosomiasis, Biomphalaria pfeifferi and Bulinus globosus from Kenya10.7717/peerj.122902167-8359https://doaj.org/article/4ae13b8ca06642ffbe04d7874fffe3c52021-11-01T00:00:00Zhttps://peerj.com/articles/12290.pdfhttps://peerj.com/articles/12290/https://doaj.org/toc/2167-8359Schistosomiasis, which infects more than 230 million people, is vectored by freshwater snails. We identified viral sequences in the transcriptomes of Biomphalaria pfeifferi (BP) and Bulinus globosus (BuG), two of the world’s most important schistosomiasis vectors in Africa. Sequences from 26 snails generated using Illumina Hi-Seq or 454 sequencing were assembled using Trinity and CAP3 and putative virus sequences were identified using a bioinformatics pipeline. Phylogenetic analyses were performed using viral RNA-dependent RNA polymerase and coat protein sequences to establish relatedness between virus sequences identified and those of known viruses. Viral sequences were identified from the entire snail holobiont, including symbionts, ingested material and organisms passively associated with the snails. Sequences derived from more than 17 different viruses were found including five near full-length genomes, most of which were small RNA viruses with positive sense RNA genomes (i.e., picorna-like viruses) and some of which are likely derived from adherent or ingested diatoms. Based on phylogenetic analysis, five of these viruses (including BPV2 and BuGV2) along with four Biomphalaria glabrata viruses reported previously, cluster with known invertebrate viruses and are putative viruses of snails. The presence of RNA sequences derived from four of these novel viruses in samples was confirmed. Identification of the genome sequences of candidate snail viruses provides a first step toward characterization of additional gastropod viruses, including from species of biomedical significance.Sijun LiuSi-Ming ZhangSarah K. BuddenborgEric S. LokerBryony C. BonningPeerJ Inc.articleSnail virusSchistosomiasis vectorVirus discoveryBiomphalaria pfeifferiBulinus globosusKenyaMedicineRENPeerJ, Vol 9, p e12290 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Snail virus Schistosomiasis vector Virus discovery Biomphalaria pfeifferi Bulinus globosus Kenya Medicine R
spellingShingle	Snail virus Schistosomiasis vector Virus discovery Biomphalaria pfeifferi Bulinus globosus Kenya Medicine R Sijun Liu Si-Ming Zhang Sarah K. Buddenborg Eric S. Loker Bryony C. Bonning Virus-derived sequences from the transcriptomes of two snail vectors of schistosomiasis, Biomphalaria pfeifferi and Bulinus globosus from Kenya
description	Schistosomiasis, which infects more than 230 million people, is vectored by freshwater snails. We identified viral sequences in the transcriptomes of Biomphalaria pfeifferi (BP) and Bulinus globosus (BuG), two of the world’s most important schistosomiasis vectors in Africa. Sequences from 26 snails generated using Illumina Hi-Seq or 454 sequencing were assembled using Trinity and CAP3 and putative virus sequences were identified using a bioinformatics pipeline. Phylogenetic analyses were performed using viral RNA-dependent RNA polymerase and coat protein sequences to establish relatedness between virus sequences identified and those of known viruses. Viral sequences were identified from the entire snail holobiont, including symbionts, ingested material and organisms passively associated with the snails. Sequences derived from more than 17 different viruses were found including five near full-length genomes, most of which were small RNA viruses with positive sense RNA genomes (i.e., picorna-like viruses) and some of which are likely derived from adherent or ingested diatoms. Based on phylogenetic analysis, five of these viruses (including BPV2 and BuGV2) along with four Biomphalaria glabrata viruses reported previously, cluster with known invertebrate viruses and are putative viruses of snails. The presence of RNA sequences derived from four of these novel viruses in samples was confirmed. Identification of the genome sequences of candidate snail viruses provides a first step toward characterization of additional gastropod viruses, including from species of biomedical significance.
format	article
author	Sijun Liu Si-Ming Zhang Sarah K. Buddenborg Eric S. Loker Bryony C. Bonning
author_facet	Sijun Liu Si-Ming Zhang Sarah K. Buddenborg Eric S. Loker Bryony C. Bonning
author_sort	Sijun Liu
title	Virus-derived sequences from the transcriptomes of two snail vectors of schistosomiasis, Biomphalaria pfeifferi and Bulinus globosus from Kenya
title_short	Virus-derived sequences from the transcriptomes of two snail vectors of schistosomiasis, Biomphalaria pfeifferi and Bulinus globosus from Kenya
title_full	Virus-derived sequences from the transcriptomes of two snail vectors of schistosomiasis, Biomphalaria pfeifferi and Bulinus globosus from Kenya
title_fullStr	Virus-derived sequences from the transcriptomes of two snail vectors of schistosomiasis, Biomphalaria pfeifferi and Bulinus globosus from Kenya
title_full_unstemmed	Virus-derived sequences from the transcriptomes of two snail vectors of schistosomiasis, Biomphalaria pfeifferi and Bulinus globosus from Kenya
title_sort	virus-derived sequences from the transcriptomes of two snail vectors of schistosomiasis, biomphalaria pfeifferi and bulinus globosus from kenya
publisher	PeerJ Inc.
publishDate	2021
url	https://doaj.org/article/4ae13b8ca06642ffbe04d7874fffe3c5
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Virus-derived sequences from the transcriptomes of two snail vectors of schistosomiasis, Biomphalaria pfeifferi and Bulinus globosus from Kenya

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