Enhanced therapeutic efficacy of drug encapsulated folic acid conjugated graphene oxide - gold nanorods for chemo-photothermal therapeutics of cervical cancer

One of the deadliest forms of malignancy affecting females worldwide alongside breast cancer is cervical cancer, which urgently calls for novel treatment approaches. Gold nanocarriers are attaining acceptance due to their inert nature, minimal side-effects, low cytotoxicity and easy fabrication. Syn...

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Autores principales:	Yu Chen, Zhiguo Luo, Chunli Chen, Ming Luo, Li Yuan
Formato:	article
Lenguaje:	EN
Publicado:	Taylor & Francis Group 2021
Materias:	bevacizumab cervical cancer graphene photothermal gold nanorod Biotechnology TP248.13-248.65
Acceso en línea:	https://doaj.org/article/4ae3f89cd96e418fa83fd887e491e1dd
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spelling	oai:doaj.org-article:4ae3f89cd96e418fa83fd887e491e1dd2021-11-04T15:00:41ZEnhanced therapeutic efficacy of drug encapsulated folic acid conjugated graphene oxide - gold nanorods for chemo-photothermal therapeutics of cervical cancer1310-28181314-353010.1080/13102818.2021.1995495https://doaj.org/article/4ae3f89cd96e418fa83fd887e491e1dd2021-01-01T00:00:00Zhttp://dx.doi.org/10.1080/13102818.2021.1995495https://doaj.org/toc/1310-2818https://doaj.org/toc/1314-3530One of the deadliest forms of malignancy affecting females worldwide alongside breast cancer is cervical cancer, which urgently calls for novel treatment approaches. Gold nanocarriers are attaining acceptance due to their inert nature, minimal side-effects, low cytotoxicity and easy fabrication. Synthesis of gold nanorods conjugated with graphene and folic acid was carried out, which were finally loaded with bevacizumab for improved therapeutics. The drug loaded folic acid conjugated graphene-gold nanorods were irradiated with non-infrared laser for improved penetration of the drugs into the specifically targeted tissue. The graphene-gold nanorods with/without drugs and irradiation at different power densities (0.25 and 0.5 W/cm2) were studied. Transmission electron microscopy, zeta potential and polydispersity index established the integrity of prepared graphene nanorods carriers. The encapsulation efficiency and drug loading of the graphene-gold nanorods were determined. In vitro cytotoxicity assay was carried out via 3‐(4,5‐dimethylthiazol‐2‐yl)‐2,5‐diphenyltetrazolium bromide, or MTT assay, with HeLa cells. There was non-significant difference between drug loading and encapsulation with irradiated and non-irradiated graphene-gold nanorods. But with irradiation the blank graphene-gold nanorods had increased cytotoxicity. The graphene-gold nanorods were released in a sustained manner as depicted by in vitro release studies. The drug loaded nanorods were also internalized by HeLa cells and distributed within the cellular compartment. We also tested drug loaded folic acid conjugated graphene-gold rods in vivo in U8GMG tumor bearing mice and results clearly indicated the irradiated ones were most efficient in suppressing tumors.Yu ChenZhiguo LuoChunli ChenMing LuoLi YuanTaylor & Francis Grouparticlebevacizumabcervical cancergraphenephotothermalgold nanorodBiotechnologyTP248.13-248.65ENBiotechnology & Biotechnological Equipment, Vol 35, Iss 1, Pp 1492-1504 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	bevacizumab cervical cancer graphene photothermal gold nanorod Biotechnology TP248.13-248.65
spellingShingle	bevacizumab cervical cancer graphene photothermal gold nanorod Biotechnology TP248.13-248.65 Yu Chen Zhiguo Luo Chunli Chen Ming Luo Li Yuan Enhanced therapeutic efficacy of drug encapsulated folic acid conjugated graphene oxide - gold nanorods for chemo-photothermal therapeutics of cervical cancer
description	One of the deadliest forms of malignancy affecting females worldwide alongside breast cancer is cervical cancer, which urgently calls for novel treatment approaches. Gold nanocarriers are attaining acceptance due to their inert nature, minimal side-effects, low cytotoxicity and easy fabrication. Synthesis of gold nanorods conjugated with graphene and folic acid was carried out, which were finally loaded with bevacizumab for improved therapeutics. The drug loaded folic acid conjugated graphene-gold nanorods were irradiated with non-infrared laser for improved penetration of the drugs into the specifically targeted tissue. The graphene-gold nanorods with/without drugs and irradiation at different power densities (0.25 and 0.5 W/cm2) were studied. Transmission electron microscopy, zeta potential and polydispersity index established the integrity of prepared graphene nanorods carriers. The encapsulation efficiency and drug loading of the graphene-gold nanorods were determined. In vitro cytotoxicity assay was carried out via 3‐(4,5‐dimethylthiazol‐2‐yl)‐2,5‐diphenyltetrazolium bromide, or MTT assay, with HeLa cells. There was non-significant difference between drug loading and encapsulation with irradiated and non-irradiated graphene-gold nanorods. But with irradiation the blank graphene-gold nanorods had increased cytotoxicity. The graphene-gold nanorods were released in a sustained manner as depicted by in vitro release studies. The drug loaded nanorods were also internalized by HeLa cells and distributed within the cellular compartment. We also tested drug loaded folic acid conjugated graphene-gold rods in vivo in U8GMG tumor bearing mice and results clearly indicated the irradiated ones were most efficient in suppressing tumors.
format	article
author	Yu Chen Zhiguo Luo Chunli Chen Ming Luo Li Yuan
author_facet	Yu Chen Zhiguo Luo Chunli Chen Ming Luo Li Yuan
author_sort	Yu Chen
title	Enhanced therapeutic efficacy of drug encapsulated folic acid conjugated graphene oxide - gold nanorods for chemo-photothermal therapeutics of cervical cancer
title_short	Enhanced therapeutic efficacy of drug encapsulated folic acid conjugated graphene oxide - gold nanorods for chemo-photothermal therapeutics of cervical cancer
title_full	Enhanced therapeutic efficacy of drug encapsulated folic acid conjugated graphene oxide - gold nanorods for chemo-photothermal therapeutics of cervical cancer
title_fullStr	Enhanced therapeutic efficacy of drug encapsulated folic acid conjugated graphene oxide - gold nanorods for chemo-photothermal therapeutics of cervical cancer
title_full_unstemmed	Enhanced therapeutic efficacy of drug encapsulated folic acid conjugated graphene oxide - gold nanorods for chemo-photothermal therapeutics of cervical cancer
title_sort	enhanced therapeutic efficacy of drug encapsulated folic acid conjugated graphene oxide - gold nanorods for chemo-photothermal therapeutics of cervical cancer
publisher	Taylor & Francis Group
publishDate	2021
url	https://doaj.org/article/4ae3f89cd96e418fa83fd887e491e1dd
work_keys_str_mv	AT yuchen enhancedtherapeuticefficacyofdrugencapsulatedfolicacidconjugatedgrapheneoxidegoldnanorodsforchemophotothermaltherapeuticsofcervicalcancer AT zhiguoluo enhancedtherapeuticefficacyofdrugencapsulatedfolicacidconjugatedgrapheneoxidegoldnanorodsforchemophotothermaltherapeuticsofcervicalcancer AT chunlichen enhancedtherapeuticefficacyofdrugencapsulatedfolicacidconjugatedgrapheneoxidegoldnanorodsforchemophotothermaltherapeuticsofcervicalcancer AT mingluo enhancedtherapeuticefficacyofdrugencapsulatedfolicacidconjugatedgrapheneoxidegoldnanorodsforchemophotothermaltherapeuticsofcervicalcancer AT liyuan enhancedtherapeuticefficacyofdrugencapsulatedfolicacidconjugatedgrapheneoxidegoldnanorodsforchemophotothermaltherapeuticsofcervicalcancer
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Enhanced therapeutic efficacy of drug encapsulated folic acid conjugated graphene oxide - gold nanorods for chemo-photothermal therapeutics of cervical cancer

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