TosR-Mediated Regulation of Adhesins and Biofilm Formation in Uropathogenic <named-content content-type="genus-species">Escherichia coli</named-content>

ABSTRACT Uropathogenic Escherichia coli strains utilize a variety of adherence factors that assist in colonization of the host urinary tract. TosA (type one secretion A) is a nonfimbrial adhesin that is predominately expressed during murine urinary tract infection (UTI), binds to kidney epithelial c...

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Autores principales:	Courtney L. Luterbach, Valerie S. Forsyth, Michael D. Engstrom, Harry L. T. Mobley
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2018
Materias:	UPEC adherence gene regulation nonfimbrial adhesin Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/4ae6257ec6974e6c81fab3d329a5cbf2
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spelling	oai:doaj.org-article:4ae6257ec6974e6c81fab3d329a5cbf22021-11-15T15:24:23ZTosR-Mediated Regulation of Adhesins and Biofilm Formation in Uropathogenic <named-content content-type="genus-species">Escherichia coli</named-content>10.1128/mSphere.00222-182379-5042https://doaj.org/article/4ae6257ec6974e6c81fab3d329a5cbf22018-06-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/mSphere.00222-18https://doaj.org/toc/2379-5042ABSTRACT Uropathogenic Escherichia coli strains utilize a variety of adherence factors that assist in colonization of the host urinary tract. TosA (type one secretion A) is a nonfimbrial adhesin that is predominately expressed during murine urinary tract infection (UTI), binds to kidney epithelial cells, and promotes survival during invasive infections. The tosRCBDAEF operon encodes the secretory machinery necessary for TosA localization to the E. coli cell surface, as well as the transcriptional regulator TosR. TosR binds upstream of the tos operon and in a concentration-dependent manner either induces or represses tosA expression. TosR is a member of the PapB family of fimbrial regulators that can participate in cross talk between fimbrial operons. TosR also binds upstream of the pap operon and suppresses PapA production. However, the scope of TosR-mediated cross talk is understudied and may be underestimated. To quantify the global effects of TosR-mediated regulation on the E. coli CFT073 genome, we induced expression of tosR, collected mRNA, and performed high-throughput RNA sequencing (RNA-Seq). These findings show that production of TosR affected the expression of genes involved with adhesins, including P, F1C, and Auf fimbriae, nitrate-nitrite transport, microcin secretion, and biofilm formation. IMPORTANCE Uropathogenic E. coli strains cause the majority of UTIs, which are the second most common bacterial infection in humans. During a UTI, bacteria adhere to cells within the urinary tract, using a number of different fimbrial and nonfimbrial adhesins. Biofilms can also develop on the surfaces of catheters, resulting in complications such as blockage. In this work, we further characterized the regulator TosR, which links both adhesin production and biofilm formation and likely plays a crucial function during UTI and disseminated infection.Courtney L. LuterbachValerie S. ForsythMichael D. EngstromHarry L. T. MobleyAmerican Society for MicrobiologyarticleUPECadherencegene regulationnonfimbrial adhesinMicrobiologyQR1-502ENmSphere, Vol 3, Iss 3 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	UPEC adherence gene regulation nonfimbrial adhesin Microbiology QR1-502
spellingShingle	UPEC adherence gene regulation nonfimbrial adhesin Microbiology QR1-502 Courtney L. Luterbach Valerie S. Forsyth Michael D. Engstrom Harry L. T. Mobley TosR-Mediated Regulation of Adhesins and Biofilm Formation in Uropathogenic <named-content content-type="genus-species">Escherichia coli</named-content>
description	ABSTRACT Uropathogenic Escherichia coli strains utilize a variety of adherence factors that assist in colonization of the host urinary tract. TosA (type one secretion A) is a nonfimbrial adhesin that is predominately expressed during murine urinary tract infection (UTI), binds to kidney epithelial cells, and promotes survival during invasive infections. The tosRCBDAEF operon encodes the secretory machinery necessary for TosA localization to the E. coli cell surface, as well as the transcriptional regulator TosR. TosR binds upstream of the tos operon and in a concentration-dependent manner either induces or represses tosA expression. TosR is a member of the PapB family of fimbrial regulators that can participate in cross talk between fimbrial operons. TosR also binds upstream of the pap operon and suppresses PapA production. However, the scope of TosR-mediated cross talk is understudied and may be underestimated. To quantify the global effects of TosR-mediated regulation on the E. coli CFT073 genome, we induced expression of tosR, collected mRNA, and performed high-throughput RNA sequencing (RNA-Seq). These findings show that production of TosR affected the expression of genes involved with adhesins, including P, F1C, and Auf fimbriae, nitrate-nitrite transport, microcin secretion, and biofilm formation. IMPORTANCE Uropathogenic E. coli strains cause the majority of UTIs, which are the second most common bacterial infection in humans. During a UTI, bacteria adhere to cells within the urinary tract, using a number of different fimbrial and nonfimbrial adhesins. Biofilms can also develop on the surfaces of catheters, resulting in complications such as blockage. In this work, we further characterized the regulator TosR, which links both adhesin production and biofilm formation and likely plays a crucial function during UTI and disseminated infection.
format	article
author	Courtney L. Luterbach Valerie S. Forsyth Michael D. Engstrom Harry L. T. Mobley
author_facet	Courtney L. Luterbach Valerie S. Forsyth Michael D. Engstrom Harry L. T. Mobley
author_sort	Courtney L. Luterbach
title	TosR-Mediated Regulation of Adhesins and Biofilm Formation in Uropathogenic <named-content content-type="genus-species">Escherichia coli</named-content>
title_short	TosR-Mediated Regulation of Adhesins and Biofilm Formation in Uropathogenic <named-content content-type="genus-species">Escherichia coli</named-content>
title_full	TosR-Mediated Regulation of Adhesins and Biofilm Formation in Uropathogenic <named-content content-type="genus-species">Escherichia coli</named-content>
title_fullStr	TosR-Mediated Regulation of Adhesins and Biofilm Formation in Uropathogenic <named-content content-type="genus-species">Escherichia coli</named-content>
title_full_unstemmed	TosR-Mediated Regulation of Adhesins and Biofilm Formation in Uropathogenic <named-content content-type="genus-species">Escherichia coli</named-content>
title_sort	tosr-mediated regulation of adhesins and biofilm formation in uropathogenic <named-content content-type="genus-species">escherichia coli</named-content>
publisher	American Society for Microbiology
publishDate	2018
url	https://doaj.org/article/4ae6257ec6974e6c81fab3d329a5cbf2
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TosR-Mediated Regulation of Adhesins and Biofilm Formation in Uropathogenic <named-content content-type="genus-species">Escherichia coli</named-content>

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