Quercetin Alleviates Osteoarthritis Progression in Rats by Suppressing Inflammation and Apoptosis via Inhibition of IRAK1/NLRP3 Signaling

Wenjun Li,1 Yeyang Wang,1 Yaqin Tang,2 Hanyu Lu,1 Yong Qi,1 Guitao Li,1 Hebei He,1 Fanglian Lu,1 Yixin Yang,1 Hongtao Sun1 1Department of Orthopedics, Guangdong Second Provincial General Hospital, Guangzhou, 510317, Guangdong Province, People’s Republic of China; 2Department of Internal Medicine, Th...

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Autores principales:	Li W, Wang Y, Tang Y, Lu H, Qi Y, Li G, He H, Lu F, Yang Y, Sun H
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2021
Materias:	quercetin osteoarthritis irak1 nlrp3 inflammation apoptosis Pathology RB1-214 Therapeutics. Pharmacology RM1-950
Acceso en línea:	https://doaj.org/article/4ae8583861a148279c6a029990ea5c17
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spelling	oai:doaj.org-article:4ae8583861a148279c6a029990ea5c172021-12-02T17:55:06ZQuercetin Alleviates Osteoarthritis Progression in Rats by Suppressing Inflammation and Apoptosis via Inhibition of IRAK1/NLRP3 Signaling1178-7031https://doaj.org/article/4ae8583861a148279c6a029990ea5c172021-07-01T00:00:00Zhttps://www.dovepress.com/quercetin-alleviates-osteoarthritis-progression-in-rats-by-suppressing-peer-reviewed-fulltext-article-JIRhttps://doaj.org/toc/1178-7031Wenjun Li,1 Yeyang Wang,1 Yaqin Tang,2 Hanyu Lu,1 Yong Qi,1 Guitao Li,1 Hebei He,1 Fanglian Lu,1 Yixin Yang,1 Hongtao Sun1 1Department of Orthopedics, Guangdong Second Provincial General Hospital, Guangzhou, 510317, Guangdong Province, People’s Republic of China; 2Department of Internal Medicine, The First Affiliated Hospital of Guangzhou University of Traditional Chinese Medicine and Pharmacology, Guangzhou, Guangdong Province, 510405, People’s Republic of ChinaCorrespondence: Hongtao SunDepartment of Orthopedics, 12th Floor, Building 3, Guangdong Second Provincial General Hospital, 466 Xingang Zhong Road, Haizhu District, Guangzhou, 510317, Guangdong Province, People’s Republic of ChinaTel +86 13808849918Email shtgd2h@126.comHanyu LuDepartment of Orthopedics, 12th Floor, Building 3, Guangdong Second Provincial General Hospital, 466 Xingang Zhong Road, Haizhu District, Guangzhou, Guangdong Province, 510317, People’s Republic of ChinaTel +86 18925077162Email luhanyu0129@163.comIntroduction: Quercetin was recently reported to help protect against osteoarthritis (OA) progression, but the molecular mechanism for that protective affect remains unclear.Methods: Here, OA model rats were intraperitoneally injected with quercetin, and the severity of cartilage damage in the rats was evaluated by H&E, Safranin O, and Toluidine blue, as well as by using the Osteoarthritis Research Society International (OARSI) Scoring System. Additionally, rat chondrocytes were treated with quercetin and then stimulated with IL-1β. The levels of pro-inflammatory cytokines (IL-1β, IL-18, and TNF-α) were detected by ELISA.Cell apoptosis was evaluated by flow cytometry and Hoechst staining. ROS levels were measured using a DCFH-DA probe. Protein expression was evaluated by Western blotting, immunohistochemical staining, and immunofluorescence.&#x00A0Results: Our data showed that quercetin attenuated the degeneration and erosion of articular cartilage, suppressed inflammation and apoptosis, and downregulated the levels of IRAK1, NLRP3, and caspase-3 expression. In vitro data showed that overexpression of NLRP3 could reverse the suppressive effect of quercetin on IL-1β-induced rat chondrocyte injuries. Importantly, rescue experiments confirmed that quercetin inhibited IL-1β-induced rat chondrocyte injuries in vitro by suppressing the IRAK1/NLRP3 signaling pathway.Conclusion: Our study indicated that quercetin inhibits IL-1β-induced inflammation and cartilage degradation by suppressing the IRAK1/NLRP3 signaling pathway.Keywords: quercetin, osteoarthritis, IRAK1, NLRP3, inflammation, apoptosisLi WWang YTang YLu HQi YLi GHe HLu FYang YSun HDove Medical Pressarticlequercetinosteoarthritisirak1nlrp3inflammationapoptosisPathologyRB1-214Therapeutics. PharmacologyRM1-950ENJournal of Inflammation Research, Vol Volume 14, Pp 3393-3403 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	quercetin osteoarthritis irak1 nlrp3 inflammation apoptosis Pathology RB1-214 Therapeutics. Pharmacology RM1-950
spellingShingle	quercetin osteoarthritis irak1 nlrp3 inflammation apoptosis Pathology RB1-214 Therapeutics. Pharmacology RM1-950 Li W Wang Y Tang Y Lu H Qi Y Li G He H Lu F Yang Y Sun H Quercetin Alleviates Osteoarthritis Progression in Rats by Suppressing Inflammation and Apoptosis via Inhibition of IRAK1/NLRP3 Signaling
description	Wenjun Li,1 Yeyang Wang,1 Yaqin Tang,2 Hanyu Lu,1 Yong Qi,1 Guitao Li,1 Hebei He,1 Fanglian Lu,1 Yixin Yang,1 Hongtao Sun1 1Department of Orthopedics, Guangdong Second Provincial General Hospital, Guangzhou, 510317, Guangdong Province, People’s Republic of China; 2Department of Internal Medicine, The First Affiliated Hospital of Guangzhou University of Traditional Chinese Medicine and Pharmacology, Guangzhou, Guangdong Province, 510405, People’s Republic of ChinaCorrespondence: Hongtao SunDepartment of Orthopedics, 12th Floor, Building 3, Guangdong Second Provincial General Hospital, 466 Xingang Zhong Road, Haizhu District, Guangzhou, 510317, Guangdong Province, People’s Republic of ChinaTel +86 13808849918Email shtgd2h@126.comHanyu LuDepartment of Orthopedics, 12th Floor, Building 3, Guangdong Second Provincial General Hospital, 466 Xingang Zhong Road, Haizhu District, Guangzhou, Guangdong Province, 510317, People’s Republic of ChinaTel +86 18925077162Email luhanyu0129@163.comIntroduction: Quercetin was recently reported to help protect against osteoarthritis (OA) progression, but the molecular mechanism for that protective affect remains unclear.Methods: Here, OA model rats were intraperitoneally injected with quercetin, and the severity of cartilage damage in the rats was evaluated by H&E, Safranin O, and Toluidine blue, as well as by using the Osteoarthritis Research Society International (OARSI) Scoring System. Additionally, rat chondrocytes were treated with quercetin and then stimulated with IL-1β. The levels of pro-inflammatory cytokines (IL-1β, IL-18, and TNF-α) were detected by ELISA.Cell apoptosis was evaluated by flow cytometry and Hoechst staining. ROS levels were measured using a DCFH-DA probe. Protein expression was evaluated by Western blotting, immunohistochemical staining, and immunofluorescence.&#x00A0Results: Our data showed that quercetin attenuated the degeneration and erosion of articular cartilage, suppressed inflammation and apoptosis, and downregulated the levels of IRAK1, NLRP3, and caspase-3 expression. In vitro data showed that overexpression of NLRP3 could reverse the suppressive effect of quercetin on IL-1β-induced rat chondrocyte injuries. Importantly, rescue experiments confirmed that quercetin inhibited IL-1β-induced rat chondrocyte injuries in vitro by suppressing the IRAK1/NLRP3 signaling pathway.Conclusion: Our study indicated that quercetin inhibits IL-1β-induced inflammation and cartilage degradation by suppressing the IRAK1/NLRP3 signaling pathway.Keywords: quercetin, osteoarthritis, IRAK1, NLRP3, inflammation, apoptosis
format	article
author	Li W Wang Y Tang Y Lu H Qi Y Li G He H Lu F Yang Y Sun H
author_facet	Li W Wang Y Tang Y Lu H Qi Y Li G He H Lu F Yang Y Sun H
author_sort	Li W
title	Quercetin Alleviates Osteoarthritis Progression in Rats by Suppressing Inflammation and Apoptosis via Inhibition of IRAK1/NLRP3 Signaling
title_short	Quercetin Alleviates Osteoarthritis Progression in Rats by Suppressing Inflammation and Apoptosis via Inhibition of IRAK1/NLRP3 Signaling
title_full	Quercetin Alleviates Osteoarthritis Progression in Rats by Suppressing Inflammation and Apoptosis via Inhibition of IRAK1/NLRP3 Signaling
title_fullStr	Quercetin Alleviates Osteoarthritis Progression in Rats by Suppressing Inflammation and Apoptosis via Inhibition of IRAK1/NLRP3 Signaling
title_full_unstemmed	Quercetin Alleviates Osteoarthritis Progression in Rats by Suppressing Inflammation and Apoptosis via Inhibition of IRAK1/NLRP3 Signaling
title_sort	quercetin alleviates osteoarthritis progression in rats by suppressing inflammation and apoptosis via inhibition of irak1/nlrp3 signaling
publisher	Dove Medical Press
publishDate	2021
url	https://doaj.org/article/4ae8583861a148279c6a029990ea5c17
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Quercetin Alleviates Osteoarthritis Progression in Rats by Suppressing Inflammation and Apoptosis via Inhibition of IRAK1/NLRP3 Signaling

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