Transcriptional profiles in Strongyloides stercoralis males reveal deviations from the Caenorhabditis sex determination model

Abstract The human and canine parasitic nematode Strongyloides stercoralis utilizes an XX/XO sex determination system, with parasitic females reproducing by mitotic parthenogenesis and free-living males and females reproducing sexually. However, the genes controlling S. stercoralis sex determination...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Damia Gonzalez Akimori, Emily J. Dalessandro, Thomas J. Nolan, Christopher R. Stieha, James B. Lok, Jonathan D. C. Stoltzfus
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/4af44b54378442aea9e52bf6c26c76ad
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:4af44b54378442aea9e52bf6c26c76ad
record_format	dspace
spelling	oai:doaj.org-article:4af44b54378442aea9e52bf6c26c76ad2021-12-02T14:27:53ZTranscriptional profiles in Strongyloides stercoralis males reveal deviations from the Caenorhabditis sex determination model10.1038/s41598-021-87478-32045-2322https://doaj.org/article/4af44b54378442aea9e52bf6c26c76ad2021-04-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-87478-3https://doaj.org/toc/2045-2322Abstract The human and canine parasitic nematode Strongyloides stercoralis utilizes an XX/XO sex determination system, with parasitic females reproducing by mitotic parthenogenesis and free-living males and females reproducing sexually. However, the genes controlling S. stercoralis sex determination and male development are unknown. We observed precocious development of rhabditiform males in permissive hosts treated with corticosteroids, suggesting that steroid hormones can regulate male development. To examine differences in transcript abundance between free-living adult males and other developmental stages, we utilized RNA-Seq. We found two clusters of S. stercoralis-specific genes encoding predicted transmembrane proteins that are only expressed in free-living males. We additionally identified homologs of several genes important for sex determination in Caenorhabditis species, including mab-3, tra-1, fem-2, and sex-1, which may have similar functions. However, we identified three paralogs of gld-1; Ss-qki-1 transcripts were highly abundant in adult males, while Ss-qki-2 and Ss-qki-3 transcripts were highly abundant in adult females. We also identified paralogs of pumilio domain-containing proteins with sex-specific transcripts. Intriguingly, her-1 appears to have been lost in several parasite lineages, and we were unable to identify homologs of tra-2 outside of Caenorhabditis species. Together, our data suggest that different mechanisms control male development in S. stercoralis and Caenorhabditis species.Damia Gonzalez AkimoriEmily J. DalessandroThomas J. NolanChristopher R. StiehaJames B. LokJonathan D. C. StoltzfusNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-16 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Damia Gonzalez Akimori Emily J. Dalessandro Thomas J. Nolan Christopher R. Stieha James B. Lok Jonathan D. C. Stoltzfus Transcriptional profiles in Strongyloides stercoralis males reveal deviations from the Caenorhabditis sex determination model
description	Abstract The human and canine parasitic nematode Strongyloides stercoralis utilizes an XX/XO sex determination system, with parasitic females reproducing by mitotic parthenogenesis and free-living males and females reproducing sexually. However, the genes controlling S. stercoralis sex determination and male development are unknown. We observed precocious development of rhabditiform males in permissive hosts treated with corticosteroids, suggesting that steroid hormones can regulate male development. To examine differences in transcript abundance between free-living adult males and other developmental stages, we utilized RNA-Seq. We found two clusters of S. stercoralis-specific genes encoding predicted transmembrane proteins that are only expressed in free-living males. We additionally identified homologs of several genes important for sex determination in Caenorhabditis species, including mab-3, tra-1, fem-2, and sex-1, which may have similar functions. However, we identified three paralogs of gld-1; Ss-qki-1 transcripts were highly abundant in adult males, while Ss-qki-2 and Ss-qki-3 transcripts were highly abundant in adult females. We also identified paralogs of pumilio domain-containing proteins with sex-specific transcripts. Intriguingly, her-1 appears to have been lost in several parasite lineages, and we were unable to identify homologs of tra-2 outside of Caenorhabditis species. Together, our data suggest that different mechanisms control male development in S. stercoralis and Caenorhabditis species.
format	article
author	Damia Gonzalez Akimori Emily J. Dalessandro Thomas J. Nolan Christopher R. Stieha James B. Lok Jonathan D. C. Stoltzfus
author_facet	Damia Gonzalez Akimori Emily J. Dalessandro Thomas J. Nolan Christopher R. Stieha James B. Lok Jonathan D. C. Stoltzfus
author_sort	Damia Gonzalez Akimori
title	Transcriptional profiles in Strongyloides stercoralis males reveal deviations from the Caenorhabditis sex determination model
title_short	Transcriptional profiles in Strongyloides stercoralis males reveal deviations from the Caenorhabditis sex determination model
title_full	Transcriptional profiles in Strongyloides stercoralis males reveal deviations from the Caenorhabditis sex determination model
title_fullStr	Transcriptional profiles in Strongyloides stercoralis males reveal deviations from the Caenorhabditis sex determination model
title_full_unstemmed	Transcriptional profiles in Strongyloides stercoralis males reveal deviations from the Caenorhabditis sex determination model
title_sort	transcriptional profiles in strongyloides stercoralis males reveal deviations from the caenorhabditis sex determination model
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/4af44b54378442aea9e52bf6c26c76ad
work_keys_str_mv	AT damiagonzalezakimori transcriptionalprofilesinstrongyloidesstercoralismalesrevealdeviationsfromthecaenorhabditissexdeterminationmodel AT emilyjdalessandro transcriptionalprofilesinstrongyloidesstercoralismalesrevealdeviationsfromthecaenorhabditissexdeterminationmodel AT thomasjnolan transcriptionalprofilesinstrongyloidesstercoralismalesrevealdeviationsfromthecaenorhabditissexdeterminationmodel AT christopherrstieha transcriptionalprofilesinstrongyloidesstercoralismalesrevealdeviationsfromthecaenorhabditissexdeterminationmodel AT jamesblok transcriptionalprofilesinstrongyloidesstercoralismalesrevealdeviationsfromthecaenorhabditissexdeterminationmodel AT jonathandcstoltzfus transcriptionalprofilesinstrongyloidesstercoralismalesrevealdeviationsfromthecaenorhabditissexdeterminationmodel
_version_	1718391249849810944

Transcriptional profiles in Strongyloides stercoralis males reveal deviations from the Caenorhabditis sex determination model

Ejemplares similares