Biosynthesis, characterization, and anticancer effect of plant-mediated silver nanoparticles using Coptis chinensis

Junwen Pei, Binfan Fu, Lifeng Jiang, Taizhen Sun Department of Integrated Traditional Chinese and Western Medicine, The Henan Cancer Hospital, Affiliated Cancer Hospital of Zhengzhou University, Zhengzhou, Henan 450008, China Background: Tremendous growth in nanotechnology has opened up new fronti...

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Autores principales:	Pei J, Fu B, Jiang L, Sun T
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2019
Materias:	Coptis Chinensis silver Nanoparticles anti-microbial lung cancer apoptosis invasion Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/4af77534b63e4d69b2a0e600483f7f50
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spelling	oai:doaj.org-article:4af77534b63e4d69b2a0e600483f7f502021-12-02T08:19:14ZBiosynthesis, characterization, and anticancer effect of plant-mediated silver nanoparticles using Coptis chinensis1178-2013https://doaj.org/article/4af77534b63e4d69b2a0e600483f7f502019-03-01T00:00:00Zhttps://www.dovepress.com/biosynthesis-characterization-and-anticancer-effect-of-plant-mediated--peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Junwen Pei, Binfan Fu, Lifeng Jiang, Taizhen Sun Department of Integrated Traditional Chinese and Western Medicine, The Henan Cancer Hospital, Affiliated Cancer Hospital of Zhengzhou University, Zhengzhou, Henan 450008, China Background: Tremendous growth in nanotechnology has opened up new frontiers in fundamental and applied aspects, including the synthesis of nanoscale matter and understanding/utilizing its exotic physicochemical and optoelectronic properties. Green-synthesis methods employing either biological microorganisms or plant extracts have emerged as a simple and alternative to chemical synthesis. Methods: In our present study, we aimed to synthesize silver nanoparticles (AgNPs) in combination with an aqueous extract of Coptis chinensis (CC) using a suitable ecofriendly green-synthesis way. Results: In our results, ultraviolet-visible spectroscopy revealed a near-absorbance peak at 450 nm, which confirmed the AgNP synthesis. The crystalline nature of the AgNPs was revealed with X-ray diffraction. Transmission electron-microscopy analysis showed spherically dispersed nanoparticles of 6–45 nm diameter. We analyzed the elementary mechanism across A549 lung carcinoma cells ahead of treatment with doses of CC-AgNPs (10 µg/mL and 25 µg/mL). The antiproliferative effect of CC-AgNPs revealed a significant decline in cell viability. Antibacterial assays with both Gram-negative (Escherichia coli) and Gram-positive (Staphylococcus aureus) bacteria exhibited a higher zone of inhibition against S. aureus. Conclusion: Furthermore, CC-AgNPs regulated apoptosis using the intrinsic pathway to inhibit A549-cell proliferation. Proliferation migration and invasion were notably inhibited by CC-AgNPs, which promoted apoptosis in lung adenocarcinoma cells by regulating the apoptotic pathway. Keywords: Coptis chinensis, silver nanoparticles, antimicrobial, lung cancer, apoptosis, invasionPei JFu BJiang LSun TDove Medical PressarticleCoptis Chinensissilver Nanoparticlesanti-microbiallung cancerapoptosisinvasionMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 14, Pp 1969-1978 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	Coptis Chinensis silver Nanoparticles anti-microbial lung cancer apoptosis invasion Medicine (General) R5-920
spellingShingle	Coptis Chinensis silver Nanoparticles anti-microbial lung cancer apoptosis invasion Medicine (General) R5-920 Pei J Fu B Jiang L Sun T Biosynthesis, characterization, and anticancer effect of plant-mediated silver nanoparticles using Coptis chinensis
description	Junwen Pei, Binfan Fu, Lifeng Jiang, Taizhen Sun Department of Integrated Traditional Chinese and Western Medicine, The Henan Cancer Hospital, Affiliated Cancer Hospital of Zhengzhou University, Zhengzhou, Henan 450008, China Background: Tremendous growth in nanotechnology has opened up new frontiers in fundamental and applied aspects, including the synthesis of nanoscale matter and understanding/utilizing its exotic physicochemical and optoelectronic properties. Green-synthesis methods employing either biological microorganisms or plant extracts have emerged as a simple and alternative to chemical synthesis. Methods: In our present study, we aimed to synthesize silver nanoparticles (AgNPs) in combination with an aqueous extract of Coptis chinensis (CC) using a suitable ecofriendly green-synthesis way. Results: In our results, ultraviolet-visible spectroscopy revealed a near-absorbance peak at 450 nm, which confirmed the AgNP synthesis. The crystalline nature of the AgNPs was revealed with X-ray diffraction. Transmission electron-microscopy analysis showed spherically dispersed nanoparticles of 6–45 nm diameter. We analyzed the elementary mechanism across A549 lung carcinoma cells ahead of treatment with doses of CC-AgNPs (10 µg/mL and 25 µg/mL). The antiproliferative effect of CC-AgNPs revealed a significant decline in cell viability. Antibacterial assays with both Gram-negative (Escherichia coli) and Gram-positive (Staphylococcus aureus) bacteria exhibited a higher zone of inhibition against S. aureus. Conclusion: Furthermore, CC-AgNPs regulated apoptosis using the intrinsic pathway to inhibit A549-cell proliferation. Proliferation migration and invasion were notably inhibited by CC-AgNPs, which promoted apoptosis in lung adenocarcinoma cells by regulating the apoptotic pathway. Keywords: Coptis chinensis, silver nanoparticles, antimicrobial, lung cancer, apoptosis, invasion
format	article
author	Pei J Fu B Jiang L Sun T
author_facet	Pei J Fu B Jiang L Sun T
author_sort	Pei J
title	Biosynthesis, characterization, and anticancer effect of plant-mediated silver nanoparticles using Coptis chinensis
title_short	Biosynthesis, characterization, and anticancer effect of plant-mediated silver nanoparticles using Coptis chinensis
title_full	Biosynthesis, characterization, and anticancer effect of plant-mediated silver nanoparticles using Coptis chinensis
title_fullStr	Biosynthesis, characterization, and anticancer effect of plant-mediated silver nanoparticles using Coptis chinensis
title_full_unstemmed	Biosynthesis, characterization, and anticancer effect of plant-mediated silver nanoparticles using Coptis chinensis
title_sort	biosynthesis, characterization, and anticancer effect of plant-mediated silver nanoparticles using coptis chinensis
publisher	Dove Medical Press
publishDate	2019
url	https://doaj.org/article/4af77534b63e4d69b2a0e600483f7f50
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Biosynthesis, characterization, and anticancer effect of plant-mediated silver nanoparticles using Coptis chinensis

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