Novel micelle formulation of curcumin for enhancing antitumor activity and inhibiting colorectal cancer stem cells

Ke Wang,1 Tao Zhang,1 Lina Liu,2 Xiaolei Wang,1 Ping Wu,1 Zhigang Chen,1 Chao Ni,1 Junshu Zhang,1 Fuqiang Hu,4 Jian Huang1,31Cancer Institute, 2Department of Pharmacy, Second Affiliated Hospital (Binjiang Branch), 3Department of Oncology, Second Affiliated Hospital, Zhejiang University School of Med...

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Autores principales:	Wang K, Zhang T, Liu L, Wang XL, Wu P, Chen ZG, Ni C, Zhang JS, Hu FQ, Huang J
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2012
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/4af9b97d96944e4591479cd2d3611c73
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spelling	oai:doaj.org-article:4af9b97d96944e4591479cd2d3611c732021-12-02T02:42:33ZNovel micelle formulation of curcumin for enhancing antitumor activity and inhibiting colorectal cancer stem cells1176-91141178-2013https://doaj.org/article/4af9b97d96944e4591479cd2d3611c732012-08-01T00:00:00Zhttp://www.dovepress.com/novel-micelle-formulation-of-curcumin-for-enhancing-antitumor-activity-a10675https://doaj.org/toc/1176-9114https://doaj.org/toc/1178-2013Ke Wang,1 Tao Zhang,1 Lina Liu,2 Xiaolei Wang,1 Ping Wu,1 Zhigang Chen,1 Chao Ni,1 Junshu Zhang,1 Fuqiang Hu,4 Jian Huang1,31Cancer Institute, 2Department of Pharmacy, Second Affiliated Hospital (Binjiang Branch), 3Department of Oncology, Second Affiliated Hospital, Zhejiang University School of Medicine, 4College of Pharmaceutical Sciences, Zhejiang University, Hangzhou, ChinaBackground and methods: Curcumin has extraordinary anticancer properties but has limited use due to its insolubility in water and instability, which leads to low systemic bioavailability. We have developed a novel nanoparticulate formulation of curcumin encapsulated in stearic acid-g-chitosan oligosaccharide (CSO-SA) polymeric micelles to overcome these hurdles.Results: The synthesized CSO-SA copolymer was able to self-assemble to form nanoscale micelles in aqueous medium. The mean diameter of the curcumin-loaded CSO-SA micelles was 114.7 nm and their mean surface potential was 18.5 mV. Curcumin-loaded CSO-SA micelles showed excellent internalization ability that increased curcumin accumulation in cancer cells. Curcumin-loaded CSO-SA micelles also had potent antiproliferative effects on primary colorectal cancer cells in vitro, resulting in about 6-fold greater inhibition compared with cells treated with a solution containing an equivalent concentration of free curcumin. Intravenous administration of curcumin-loaded CSO-SA micelles marginally suppressed tumor growth but did not increase cytotoxicity to mice, as confirmed by no change in body weight. Most importantly, curcumin-loaded CSO-SA micelles were effective for inhibiting subpopulations of CD44+/CD24+ cells (putative colorectal cancer stem cell markers) both in vitro and in vivo.Conclusion: The present study identifies an effective and safe means of using curcumin-loaded CSO-SA micelles for cancer therapy.Keywords: chitosan oligosaccharide, polymeric micelle, curcumin, drug delivery, colorectal cancer, cancer stem cellsWang KZhang TLiu LWang XLWu PChen ZGNi CZhang JSHu FQHuang JDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2012, Iss default, Pp 4487-4497 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Wang K Zhang T Liu L Wang XL Wu P Chen ZG Ni C Zhang JS Hu FQ Huang J Novel micelle formulation of curcumin for enhancing antitumor activity and inhibiting colorectal cancer stem cells
description	Ke Wang,1 Tao Zhang,1 Lina Liu,2 Xiaolei Wang,1 Ping Wu,1 Zhigang Chen,1 Chao Ni,1 Junshu Zhang,1 Fuqiang Hu,4 Jian Huang1,31Cancer Institute, 2Department of Pharmacy, Second Affiliated Hospital (Binjiang Branch), 3Department of Oncology, Second Affiliated Hospital, Zhejiang University School of Medicine, 4College of Pharmaceutical Sciences, Zhejiang University, Hangzhou, ChinaBackground and methods: Curcumin has extraordinary anticancer properties but has limited use due to its insolubility in water and instability, which leads to low systemic bioavailability. We have developed a novel nanoparticulate formulation of curcumin encapsulated in stearic acid-g-chitosan oligosaccharide (CSO-SA) polymeric micelles to overcome these hurdles.Results: The synthesized CSO-SA copolymer was able to self-assemble to form nanoscale micelles in aqueous medium. The mean diameter of the curcumin-loaded CSO-SA micelles was 114.7 nm and their mean surface potential was 18.5 mV. Curcumin-loaded CSO-SA micelles showed excellent internalization ability that increased curcumin accumulation in cancer cells. Curcumin-loaded CSO-SA micelles also had potent antiproliferative effects on primary colorectal cancer cells in vitro, resulting in about 6-fold greater inhibition compared with cells treated with a solution containing an equivalent concentration of free curcumin. Intravenous administration of curcumin-loaded CSO-SA micelles marginally suppressed tumor growth but did not increase cytotoxicity to mice, as confirmed by no change in body weight. Most importantly, curcumin-loaded CSO-SA micelles were effective for inhibiting subpopulations of CD44+/CD24+ cells (putative colorectal cancer stem cell markers) both in vitro and in vivo.Conclusion: The present study identifies an effective and safe means of using curcumin-loaded CSO-SA micelles for cancer therapy.Keywords: chitosan oligosaccharide, polymeric micelle, curcumin, drug delivery, colorectal cancer, cancer stem cells
format	article
author	Wang K Zhang T Liu L Wang XL Wu P Chen ZG Ni C Zhang JS Hu FQ Huang J
author_facet	Wang K Zhang T Liu L Wang XL Wu P Chen ZG Ni C Zhang JS Hu FQ Huang J
author_sort	Wang K
title	Novel micelle formulation of curcumin for enhancing antitumor activity and inhibiting colorectal cancer stem cells
title_short	Novel micelle formulation of curcumin for enhancing antitumor activity and inhibiting colorectal cancer stem cells
title_full	Novel micelle formulation of curcumin for enhancing antitumor activity and inhibiting colorectal cancer stem cells
title_fullStr	Novel micelle formulation of curcumin for enhancing antitumor activity and inhibiting colorectal cancer stem cells
title_full_unstemmed	Novel micelle formulation of curcumin for enhancing antitumor activity and inhibiting colorectal cancer stem cells
title_sort	novel micelle formulation of curcumin for enhancing antitumor activity and inhibiting colorectal cancer stem cells
publisher	Dove Medical Press
publishDate	2012
url	https://doaj.org/article/4af9b97d96944e4591479cd2d3611c73
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Novel micelle formulation of curcumin for enhancing antitumor activity and inhibiting colorectal cancer stem cells

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