Region of Interest analysis using mass spectrometry imaging of mitochondrial and sarcomeric proteins in acute cardiac infarction tissue

Abstract Matrix-assisted laser desorption ionization image mass spectrometry (MALDI-IMS) has been developed for the identification of peptides in various tissues. The MALDI-IMS signal distribution patterns and quantification of the signal intensities of the regions of interest (ROI) with healthy reg...

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Autores principales:	Yuka Yajima, Takuya Hiratsuka, Yu Kakimoto, Shuichiro Ogawa, Keisuke Shima, Yuzo Yamazaki, Kenichi Yoshikawa, Keiji Tamaki, Tatsuaki Tsuruyama
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/4b093f2b4625489cbe3271778c9bdef1
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spelling	oai:doaj.org-article:4b093f2b4625489cbe3271778c9bdef12021-12-02T11:41:02ZRegion of Interest analysis using mass spectrometry imaging of mitochondrial and sarcomeric proteins in acute cardiac infarction tissue10.1038/s41598-018-25817-72045-2322https://doaj.org/article/4b093f2b4625489cbe3271778c9bdef12018-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-25817-7https://doaj.org/toc/2045-2322Abstract Matrix-assisted laser desorption ionization image mass spectrometry (MALDI-IMS) has been developed for the identification of peptides in various tissues. The MALDI-IMS signal distribution patterns and quantification of the signal intensities of the regions of interest (ROI) with healthy regions were compared for identification of the disease specific biomarkers. We performed a new ROI analysis using the conventional t-test and data number independent Cohen’s d-value analysis. Using these techniques, we analysed heart tissues after acute myocardial infarction (AMI). As a result, IMS signals of mitochondrial adenosine triphosphate synthase alpha subunit (ATP5A), myosin-6/7(MYH6/7), aortic actin, and the myosin light chain 3 (MYL3) were identified in the infarcted region. In particular, the signals of MYH7 are significantly greater in the infarcted region using ROI analysis. ROI analysis using MALDI-IMS may be a promising technique for the identification of biomarkers for pathological studies that involve the comparison of diseased and control areas.Yuka YajimaTakuya HiratsukaYu KakimotoShuichiro OgawaKeisuke ShimaYuzo YamazakiKenichi YoshikawaKeiji TamakiTatsuaki TsuruyamaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-10 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Yuka Yajima Takuya Hiratsuka Yu Kakimoto Shuichiro Ogawa Keisuke Shima Yuzo Yamazaki Kenichi Yoshikawa Keiji Tamaki Tatsuaki Tsuruyama Region of Interest analysis using mass spectrometry imaging of mitochondrial and sarcomeric proteins in acute cardiac infarction tissue
description	Abstract Matrix-assisted laser desorption ionization image mass spectrometry (MALDI-IMS) has been developed for the identification of peptides in various tissues. The MALDI-IMS signal distribution patterns and quantification of the signal intensities of the regions of interest (ROI) with healthy regions were compared for identification of the disease specific biomarkers. We performed a new ROI analysis using the conventional t-test and data number independent Cohen’s d-value analysis. Using these techniques, we analysed heart tissues after acute myocardial infarction (AMI). As a result, IMS signals of mitochondrial adenosine triphosphate synthase alpha subunit (ATP5A), myosin-6/7(MYH6/7), aortic actin, and the myosin light chain 3 (MYL3) were identified in the infarcted region. In particular, the signals of MYH7 are significantly greater in the infarcted region using ROI analysis. ROI analysis using MALDI-IMS may be a promising technique for the identification of biomarkers for pathological studies that involve the comparison of diseased and control areas.
format	article
author	Yuka Yajima Takuya Hiratsuka Yu Kakimoto Shuichiro Ogawa Keisuke Shima Yuzo Yamazaki Kenichi Yoshikawa Keiji Tamaki Tatsuaki Tsuruyama
author_facet	Yuka Yajima Takuya Hiratsuka Yu Kakimoto Shuichiro Ogawa Keisuke Shima Yuzo Yamazaki Kenichi Yoshikawa Keiji Tamaki Tatsuaki Tsuruyama
author_sort	Yuka Yajima
title	Region of Interest analysis using mass spectrometry imaging of mitochondrial and sarcomeric proteins in acute cardiac infarction tissue
title_short	Region of Interest analysis using mass spectrometry imaging of mitochondrial and sarcomeric proteins in acute cardiac infarction tissue
title_full	Region of Interest analysis using mass spectrometry imaging of mitochondrial and sarcomeric proteins in acute cardiac infarction tissue
title_fullStr	Region of Interest analysis using mass spectrometry imaging of mitochondrial and sarcomeric proteins in acute cardiac infarction tissue
title_full_unstemmed	Region of Interest analysis using mass spectrometry imaging of mitochondrial and sarcomeric proteins in acute cardiac infarction tissue
title_sort	region of interest analysis using mass spectrometry imaging of mitochondrial and sarcomeric proteins in acute cardiac infarction tissue
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/4b093f2b4625489cbe3271778c9bdef1
work_keys_str_mv	AT yukayajima regionofinterestanalysisusingmassspectrometryimagingofmitochondrialandsarcomericproteinsinacutecardiacinfarctiontissue AT takuyahiratsuka regionofinterestanalysisusingmassspectrometryimagingofmitochondrialandsarcomericproteinsinacutecardiacinfarctiontissue AT yukakimoto regionofinterestanalysisusingmassspectrometryimagingofmitochondrialandsarcomericproteinsinacutecardiacinfarctiontissue AT shuichiroogawa regionofinterestanalysisusingmassspectrometryimagingofmitochondrialandsarcomericproteinsinacutecardiacinfarctiontissue AT keisukeshima regionofinterestanalysisusingmassspectrometryimagingofmitochondrialandsarcomericproteinsinacutecardiacinfarctiontissue AT yuzoyamazaki regionofinterestanalysisusingmassspectrometryimagingofmitochondrialandsarcomericproteinsinacutecardiacinfarctiontissue AT kenichiyoshikawa regionofinterestanalysisusingmassspectrometryimagingofmitochondrialandsarcomericproteinsinacutecardiacinfarctiontissue AT keijitamaki regionofinterestanalysisusingmassspectrometryimagingofmitochondrialandsarcomericproteinsinacutecardiacinfarctiontissue AT tatsuakitsuruyama regionofinterestanalysisusingmassspectrometryimagingofmitochondrialandsarcomericproteinsinacutecardiacinfarctiontissue
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Region of Interest analysis using mass spectrometry imaging of mitochondrial and sarcomeric proteins in acute cardiac infarction tissue

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