Analysis on MTGase catalysed cross-linked products of Ara h 2: structure and immunoreactivity

Peanut is ranked among the eight major food allergens. Ara h 2 is its major allergen, recognized by more than 90% of serum IgE from peanut-allergic patients. Cross-linking catalysed by microbial transglutaminase (MTGase) is proved to be feasible to decrease the allergenicity of Ara h 2. The crosslin...

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Autores principales:	Xuejiao Chang, Zhihua Wu, Ruifang Zhao, Ying Zhang, Xin Li, Anshu Yang, Ping Tong, Hongbing Chen
Formato:	article
Lenguaje:	EN
Publicado:	Taylor & Francis Group 2018
Materias:	ara h 2 crosslinking structure immunoreactivity potential allergenicity Agriculture (General) S1-972 Immunologic diseases. Allergy RC581-607
Acceso en línea:	https://doaj.org/article/4b0b35bc18e341db94e56ef1036c9c80
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spelling	oai:doaj.org-article:4b0b35bc18e341db94e56ef1036c9c802021-11-26T11:19:47ZAnalysis on MTGase catalysed cross-linked products of Ara h 2: structure and immunoreactivity0954-01051465-344310.1080/09540105.2018.1529739https://doaj.org/article/4b0b35bc18e341db94e56ef1036c9c802018-01-01T00:00:00Zhttp://dx.doi.org/10.1080/09540105.2018.1529739https://doaj.org/toc/0954-0105https://doaj.org/toc/1465-3443Peanut is ranked among the eight major food allergens. Ara h 2 is its major allergen, recognized by more than 90% of serum IgE from peanut-allergic patients. Cross-linking catalysed by microbial transglutaminase (MTGase) is proved to be feasible to decrease the allergenicity of Ara h 2. The crosslinking reaction occurred both inter- and intra-molecular could gain products with different molecular weights. In this study, after MTGase catalysed crosslinking, the products were separated into low molecular weight part of Ara h 2 (LP-Ara h 2) and high molecular weight part of Ara h 2 (HP-Ara h 2), and the structure, digestibility, IgG and IgE binding capability of each part were analysed. Compared with LP-Ara h 2, HP-Ara h 2 was found to have looser structure, higher digestion rate and corresponding lower immunogenicity. By controlling the reaction condition to get different products, the protein desensitization processes would get a better result.Xuejiao ChangZhihua WuRuifang ZhaoYing ZhangXin LiAnshu YangPing TongHongbing ChenTaylor & Francis Grouparticleara h 2crosslinkingstructureimmunoreactivitypotential allergenicityAgriculture (General)S1-972Immunologic diseases. AllergyRC581-607ENFood and Agricultural Immunology, Vol 29, Iss 1, Pp 1197-1208 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	ara h 2 crosslinking structure immunoreactivity potential allergenicity Agriculture (General) S1-972 Immunologic diseases. Allergy RC581-607
spellingShingle	ara h 2 crosslinking structure immunoreactivity potential allergenicity Agriculture (General) S1-972 Immunologic diseases. Allergy RC581-607 Xuejiao Chang Zhihua Wu Ruifang Zhao Ying Zhang Xin Li Anshu Yang Ping Tong Hongbing Chen Analysis on MTGase catalysed cross-linked products of Ara h 2: structure and immunoreactivity
description	Peanut is ranked among the eight major food allergens. Ara h 2 is its major allergen, recognized by more than 90% of serum IgE from peanut-allergic patients. Cross-linking catalysed by microbial transglutaminase (MTGase) is proved to be feasible to decrease the allergenicity of Ara h 2. The crosslinking reaction occurred both inter- and intra-molecular could gain products with different molecular weights. In this study, after MTGase catalysed crosslinking, the products were separated into low molecular weight part of Ara h 2 (LP-Ara h 2) and high molecular weight part of Ara h 2 (HP-Ara h 2), and the structure, digestibility, IgG and IgE binding capability of each part were analysed. Compared with LP-Ara h 2, HP-Ara h 2 was found to have looser structure, higher digestion rate and corresponding lower immunogenicity. By controlling the reaction condition to get different products, the protein desensitization processes would get a better result.
format	article
author	Xuejiao Chang Zhihua Wu Ruifang Zhao Ying Zhang Xin Li Anshu Yang Ping Tong Hongbing Chen
author_facet	Xuejiao Chang Zhihua Wu Ruifang Zhao Ying Zhang Xin Li Anshu Yang Ping Tong Hongbing Chen
author_sort	Xuejiao Chang
title	Analysis on MTGase catalysed cross-linked products of Ara h 2: structure and immunoreactivity
title_short	Analysis on MTGase catalysed cross-linked products of Ara h 2: structure and immunoreactivity
title_full	Analysis on MTGase catalysed cross-linked products of Ara h 2: structure and immunoreactivity
title_fullStr	Analysis on MTGase catalysed cross-linked products of Ara h 2: structure and immunoreactivity
title_full_unstemmed	Analysis on MTGase catalysed cross-linked products of Ara h 2: structure and immunoreactivity
title_sort	analysis on mtgase catalysed cross-linked products of ara h 2: structure and immunoreactivity
publisher	Taylor & Francis Group
publishDate	2018
url	https://doaj.org/article/4b0b35bc18e341db94e56ef1036c9c80
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Analysis on MTGase catalysed cross-linked products of Ara h 2: structure and immunoreactivity

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