First Genomic Evidence of Dual African Swine Fever Virus Infection: Case Report from Recent and Historical Outbreaks in Sardinia

African swine fever virus (ASFV) is one of the pathogens of highest concern worldwide. Despite different virus lineages co-circulating in several areas, dual infections in the same animal have been rarely observed, suggesting that ASF superinfections are infrequent events. Here we present the first...

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Autores principales:	Mariangela Stefania Fiori, Luca Ferretti, Matteo Floris, Federica Loi, Antonello Di Nardo, Anna Maria Sechi, Anna Ladu, Graziella Puggioni, Daria Sanna, Fabio Scarpa, Maria Luisa Sanna, Maria Paola Madrau, Claudia Torresi, Roberto Sirica, Eloisa Evangelista, Annalisa Oggiano, Silvia Dei Giudici
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	African swine fever dual infection phylogenetic analysis whole genome sequence Sardinia Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/4b0bd290334840f68f3d7c79111acc95
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spelling	oai:doaj.org-article:4b0bd290334840f68f3d7c79111acc952021-11-25T19:12:43ZFirst Genomic Evidence of Dual African Swine Fever Virus Infection: Case Report from Recent and Historical Outbreaks in Sardinia10.3390/v131121451999-4915https://doaj.org/article/4b0bd290334840f68f3d7c79111acc952021-10-01T00:00:00Zhttps://www.mdpi.com/1999-4915/13/11/2145https://doaj.org/toc/1999-4915African swine fever virus (ASFV) is one of the pathogens of highest concern worldwide. Despite different virus lineages co-circulating in several areas, dual infections in the same animal have been rarely observed, suggesting that ASF superinfections are infrequent events. Here we present the first genome-wide detection and analysis of two intragenotype dual ASFV infections. The dual infections have been detected in a hunted wild boar and in a pig carcass, both infected by ASFV genotype I in Sardinia in 1984 and 2018, respectively. We characterize the genetic differences between the two sequences, their intra-host frequency, and their phylogenetic relationship among fully sequenced ASFV strains from Sardinia. Both dual infections involve pairs of closely related but different viruses that were circulating in Sardinia in the same period. The results imply that dual ASFV infections or similar ASFV strains are more common than expected, especially in ASF endemic areas, albeit difficult to detect.Mariangela Stefania FioriLuca FerrettiMatteo FlorisFederica LoiAntonello Di NardoAnna Maria SechiAnna LaduGraziella PuggioniDaria SannaFabio ScarpaMaria Luisa SannaMaria Paola MadrauClaudia TorresiRoberto SiricaEloisa EvangelistaAnnalisa OggianoSilvia Dei GiudiciMDPI AGarticleAfrican swine feverdual infectionphylogenetic analysiswhole genome sequenceSardiniaMicrobiologyQR1-502ENViruses, Vol 13, Iss 2145, p 2145 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	African swine fever dual infection phylogenetic analysis whole genome sequence Sardinia Microbiology QR1-502
spellingShingle	African swine fever dual infection phylogenetic analysis whole genome sequence Sardinia Microbiology QR1-502 Mariangela Stefania Fiori Luca Ferretti Matteo Floris Federica Loi Antonello Di Nardo Anna Maria Sechi Anna Ladu Graziella Puggioni Daria Sanna Fabio Scarpa Maria Luisa Sanna Maria Paola Madrau Claudia Torresi Roberto Sirica Eloisa Evangelista Annalisa Oggiano Silvia Dei Giudici First Genomic Evidence of Dual African Swine Fever Virus Infection: Case Report from Recent and Historical Outbreaks in Sardinia
description	African swine fever virus (ASFV) is one of the pathogens of highest concern worldwide. Despite different virus lineages co-circulating in several areas, dual infections in the same animal have been rarely observed, suggesting that ASF superinfections are infrequent events. Here we present the first genome-wide detection and analysis of two intragenotype dual ASFV infections. The dual infections have been detected in a hunted wild boar and in a pig carcass, both infected by ASFV genotype I in Sardinia in 1984 and 2018, respectively. We characterize the genetic differences between the two sequences, their intra-host frequency, and their phylogenetic relationship among fully sequenced ASFV strains from Sardinia. Both dual infections involve pairs of closely related but different viruses that were circulating in Sardinia in the same period. The results imply that dual ASFV infections or similar ASFV strains are more common than expected, especially in ASF endemic areas, albeit difficult to detect.
format	article
author	Mariangela Stefania Fiori Luca Ferretti Matteo Floris Federica Loi Antonello Di Nardo Anna Maria Sechi Anna Ladu Graziella Puggioni Daria Sanna Fabio Scarpa Maria Luisa Sanna Maria Paola Madrau Claudia Torresi Roberto Sirica Eloisa Evangelista Annalisa Oggiano Silvia Dei Giudici
author_facet	Mariangela Stefania Fiori Luca Ferretti Matteo Floris Federica Loi Antonello Di Nardo Anna Maria Sechi Anna Ladu Graziella Puggioni Daria Sanna Fabio Scarpa Maria Luisa Sanna Maria Paola Madrau Claudia Torresi Roberto Sirica Eloisa Evangelista Annalisa Oggiano Silvia Dei Giudici
author_sort	Mariangela Stefania Fiori
title	First Genomic Evidence of Dual African Swine Fever Virus Infection: Case Report from Recent and Historical Outbreaks in Sardinia
title_short	First Genomic Evidence of Dual African Swine Fever Virus Infection: Case Report from Recent and Historical Outbreaks in Sardinia
title_full	First Genomic Evidence of Dual African Swine Fever Virus Infection: Case Report from Recent and Historical Outbreaks in Sardinia
title_fullStr	First Genomic Evidence of Dual African Swine Fever Virus Infection: Case Report from Recent and Historical Outbreaks in Sardinia
title_full_unstemmed	First Genomic Evidence of Dual African Swine Fever Virus Infection: Case Report from Recent and Historical Outbreaks in Sardinia
title_sort	first genomic evidence of dual african swine fever virus infection: case report from recent and historical outbreaks in sardinia
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/4b0bd290334840f68f3d7c79111acc95
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First Genomic Evidence of Dual African Swine Fever Virus Infection: Case Report from Recent and Historical Outbreaks in Sardinia

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