Development of PCR-based identification of Salmonella enterica serovars

The aim of the study was to evaluate and adapt the PCR-based protocol that utilizes the developed serotype-specific primers to identify Salmonella enterica species and its serotypes that are most frequently isolated from poultry samples in Vojvodina. Using the slide agglutination test, 64 and 33 out...

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Autores principales:	Kiskároly Ferenc, Morić Ivana, Đokić Lidija, Vasiljević Branka, Šenerović Lidija, Mišić Dušan
Formato:	article
Lenguaje:	EN
Publicado:	Sciendo 2017
Materias:	salmonella poultry multiplex pcr identification taxonomy Veterinary medicine SF600-1100
Acceso en línea:	https://doaj.org/article/4b0cad86f7034ed79e3bcd71ab7c42c3
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spelling	oai:doaj.org-article:4b0cad86f7034ed79e3bcd71ab7c42c32021-11-17T21:27:51ZDevelopment of PCR-based identification of Salmonella enterica serovars1820-744810.1515/acve-2017-0022https://doaj.org/article/4b0cad86f7034ed79e3bcd71ab7c42c32017-06-01T00:00:00Zhttps://doi.org/10.1515/acve-2017-0022https://doaj.org/toc/1820-7448The aim of the study was to evaluate and adapt the PCR-based protocol that utilizes the developed serotype-specific primers to identify Salmonella enterica species and its serotypes that are most frequently isolated from poultry samples in Vojvodina. Using the slide agglutination test, 64 and 33 out of 107 Salmonella isolates were identified as S. Infantis and S. Enteritidis, respectively, while ten isolates were identified as eight different Salmonella serovars. Using the same isolates, presence of 993-bp (bcfC gene), 636-bp (steB gene) and 293-bp (sdf locus) amplicons in multiplex PCR unambiguously identified 31 isolates as S. Enteritidis. Two isolates identified as Enteritidis in slide agglutination test were not identified as such in PCR-based approach since they both were missing 293-bp long PCR product. Thirty-nine isolates produced a 727-bp amplicon in the specific simplex PCR, and thus were identified as S. Infantis. The greatest discrepancy in comparison to the results of conventional serotyping has been observed in the case of S. Infantis, since 25 more isolates were noted as S. Infantis by conventional serotyping. Seven isolates, with unexpected PCR profiles stayed unidentified by molecular typing, although they were serotyped as S. Typhimurium (1) and S. Infantis (6). S. Gallinarum serovar has to be additionally confirmed, since it shares the same PCR profile with S. Livingstone. Clearly, PCR-based identification has to be thoroughly checked, verified and adapted if it is to be applied as the routine identification protocol.Kiskároly FerencMorić IvanaĐokić LidijaVasiljević BrankaŠenerović LidijaMišić DušanSciendoarticlesalmonellapoultrymultiplex pcridentificationtaxonomyVeterinary medicineSF600-1100ENActa Veterinaria, Vol 67, Iss 2, Pp 271-284 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	salmonella poultry multiplex pcr identification taxonomy Veterinary medicine SF600-1100
spellingShingle	salmonella poultry multiplex pcr identification taxonomy Veterinary medicine SF600-1100 Kiskároly Ferenc Morić Ivana Đokić Lidija Vasiljević Branka Šenerović Lidija Mišić Dušan Development of PCR-based identification of Salmonella enterica serovars
description	The aim of the study was to evaluate and adapt the PCR-based protocol that utilizes the developed serotype-specific primers to identify Salmonella enterica species and its serotypes that are most frequently isolated from poultry samples in Vojvodina. Using the slide agglutination test, 64 and 33 out of 107 Salmonella isolates were identified as S. Infantis and S. Enteritidis, respectively, while ten isolates were identified as eight different Salmonella serovars. Using the same isolates, presence of 993-bp (bcfC gene), 636-bp (steB gene) and 293-bp (sdf locus) amplicons in multiplex PCR unambiguously identified 31 isolates as S. Enteritidis. Two isolates identified as Enteritidis in slide agglutination test were not identified as such in PCR-based approach since they both were missing 293-bp long PCR product. Thirty-nine isolates produced a 727-bp amplicon in the specific simplex PCR, and thus were identified as S. Infantis. The greatest discrepancy in comparison to the results of conventional serotyping has been observed in the case of S. Infantis, since 25 more isolates were noted as S. Infantis by conventional serotyping. Seven isolates, with unexpected PCR profiles stayed unidentified by molecular typing, although they were serotyped as S. Typhimurium (1) and S. Infantis (6). S. Gallinarum serovar has to be additionally confirmed, since it shares the same PCR profile with S. Livingstone. Clearly, PCR-based identification has to be thoroughly checked, verified and adapted if it is to be applied as the routine identification protocol.
format	article
author	Kiskároly Ferenc Morić Ivana Đokić Lidija Vasiljević Branka Šenerović Lidija Mišić Dušan
author_facet	Kiskároly Ferenc Morić Ivana Đokić Lidija Vasiljević Branka Šenerović Lidija Mišić Dušan
author_sort	Kiskároly Ferenc
title	Development of PCR-based identification of Salmonella enterica serovars
title_short	Development of PCR-based identification of Salmonella enterica serovars
title_full	Development of PCR-based identification of Salmonella enterica serovars
title_fullStr	Development of PCR-based identification of Salmonella enterica serovars
title_full_unstemmed	Development of PCR-based identification of Salmonella enterica serovars
title_sort	development of pcr-based identification of salmonella enterica serovars
publisher	Sciendo
publishDate	2017
url	https://doaj.org/article/4b0cad86f7034ed79e3bcd71ab7c42c3
work_keys_str_mv	AT kiskarolyferenc developmentofpcrbasedidentificationofsalmonellaentericaserovars AT moricivana developmentofpcrbasedidentificationofsalmonellaentericaserovars AT đokiclidija developmentofpcrbasedidentificationofsalmonellaentericaserovars AT vasiljevicbranka developmentofpcrbasedidentificationofsalmonellaentericaserovars AT seneroviclidija developmentofpcrbasedidentificationofsalmonellaentericaserovars AT misicdusan developmentofpcrbasedidentificationofsalmonellaentericaserovars
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Development of PCR-based identification of Salmonella enterica serovars

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