Development of a rapid scabies immunodiagnostic assay based on transcriptomic analysis of Sarcoptes scabiei var. nyctereutis

Abstract Scabies is a highly contagious skin disease caused by the mite Sarcoptes scabiei that affects many mammals. However, the sensitivity of traditional tests for scabies diagnosis in humans is less than 50%. To simplify the diagnosis of scabies, methods that are simple, sensitive, specific, and...

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Autores principales:	Teruo Akuta, Daisuke Minegishi, Nobuhide Kido, Keitaro Imaizumi, Shinji Nakaoka, Shin-Ichiro Tachibana, Kenji Hikosaka, Fumi Hori, Masataka, Nakagawa, Chiaki Sakuma, Yuki Oouchi, Yu Nakajima, Sohei Tanaka, Tomoko Omiya, Kouki Morikaku, Minori Kawahara, Yoshifumi Tada, Hiroshi Tarui, Takafumi Ueda, Takane Kikuchi-Ueda, Yasuo Ono
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/4b1a1fd9236441328e4c8ffb0f0a9788
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spelling	oai:doaj.org-article:4b1a1fd9236441328e4c8ffb0f0a97882021-12-02T11:39:44ZDevelopment of a rapid scabies immunodiagnostic assay based on transcriptomic analysis of Sarcoptes scabiei var. nyctereutis10.1038/s41598-021-85290-72045-2322https://doaj.org/article/4b1a1fd9236441328e4c8ffb0f0a97882021-03-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-85290-7https://doaj.org/toc/2045-2322Abstract Scabies is a highly contagious skin disease caused by the mite Sarcoptes scabiei that affects many mammals. However, the sensitivity of traditional tests for scabies diagnosis in humans is less than 50%. To simplify the diagnosis of scabies, methods that are simple, sensitive, specific, and cost-effective are required. We developed an immunodiagnostic test based on S. scabiei var. nyctereutis RNA-seq data collected from Japanese raccoon dogs with sarcoptic mange. Three candidate antigens—a highly expressed hypothetical protein “QR98_0091190,” another mite allergen known as “SMIPP-Cc,” and an abundant “vitellogenin-like protein”—were evaluated by western-blot analysis. A lateral flow immunoassay, using specific antibodies against the vitellogenin-like protein, successfully detected scabies in the skin flakes of S. scabiei-infected raccoon dogs. This assay can potentially diagnose scabies more accurately in wildlife, as well as in humans.Teruo AkutaDaisuke MinegishiNobuhide KidoKeitaro ImaizumiShinji NakaokaShin-Ichiro TachibanaKenji HikosakaFumi HoriMasatakaNakagawaChiaki SakumaYuki OouchiYu NakajimaSohei TanakaTomoko OmiyaKouki MorikakuMinori KawaharaYoshifumi TadaHiroshi TaruiTakafumi UedaTakane Kikuchi-UedaYasuo OnoNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-13 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Teruo Akuta Daisuke Minegishi Nobuhide Kido Keitaro Imaizumi Shinji Nakaoka Shin-Ichiro Tachibana Kenji Hikosaka Fumi Hori Masataka Nakagawa Chiaki Sakuma Yuki Oouchi Yu Nakajima Sohei Tanaka Tomoko Omiya Kouki Morikaku Minori Kawahara Yoshifumi Tada Hiroshi Tarui Takafumi Ueda Takane Kikuchi-Ueda Yasuo Ono Development of a rapid scabies immunodiagnostic assay based on transcriptomic analysis of Sarcoptes scabiei var. nyctereutis
description	Abstract Scabies is a highly contagious skin disease caused by the mite Sarcoptes scabiei that affects many mammals. However, the sensitivity of traditional tests for scabies diagnosis in humans is less than 50%. To simplify the diagnosis of scabies, methods that are simple, sensitive, specific, and cost-effective are required. We developed an immunodiagnostic test based on S. scabiei var. nyctereutis RNA-seq data collected from Japanese raccoon dogs with sarcoptic mange. Three candidate antigens—a highly expressed hypothetical protein “QR98_0091190,” another mite allergen known as “SMIPP-Cc,” and an abundant “vitellogenin-like protein”—were evaluated by western-blot analysis. A lateral flow immunoassay, using specific antibodies against the vitellogenin-like protein, successfully detected scabies in the skin flakes of S. scabiei-infected raccoon dogs. This assay can potentially diagnose scabies more accurately in wildlife, as well as in humans.
format	article
author	Teruo Akuta Daisuke Minegishi Nobuhide Kido Keitaro Imaizumi Shinji Nakaoka Shin-Ichiro Tachibana Kenji Hikosaka Fumi Hori Masataka Nakagawa Chiaki Sakuma Yuki Oouchi Yu Nakajima Sohei Tanaka Tomoko Omiya Kouki Morikaku Minori Kawahara Yoshifumi Tada Hiroshi Tarui Takafumi Ueda Takane Kikuchi-Ueda Yasuo Ono
author_facet	Teruo Akuta Daisuke Minegishi Nobuhide Kido Keitaro Imaizumi Shinji Nakaoka Shin-Ichiro Tachibana Kenji Hikosaka Fumi Hori Masataka Nakagawa Chiaki Sakuma Yuki Oouchi Yu Nakajima Sohei Tanaka Tomoko Omiya Kouki Morikaku Minori Kawahara Yoshifumi Tada Hiroshi Tarui Takafumi Ueda Takane Kikuchi-Ueda Yasuo Ono
author_sort	Teruo Akuta
title	Development of a rapid scabies immunodiagnostic assay based on transcriptomic analysis of Sarcoptes scabiei var. nyctereutis
title_short	Development of a rapid scabies immunodiagnostic assay based on transcriptomic analysis of Sarcoptes scabiei var. nyctereutis
title_full	Development of a rapid scabies immunodiagnostic assay based on transcriptomic analysis of Sarcoptes scabiei var. nyctereutis
title_fullStr	Development of a rapid scabies immunodiagnostic assay based on transcriptomic analysis of Sarcoptes scabiei var. nyctereutis
title_full_unstemmed	Development of a rapid scabies immunodiagnostic assay based on transcriptomic analysis of Sarcoptes scabiei var. nyctereutis
title_sort	development of a rapid scabies immunodiagnostic assay based on transcriptomic analysis of sarcoptes scabiei var. nyctereutis
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/4b1a1fd9236441328e4c8ffb0f0a9788
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Development of a rapid scabies immunodiagnostic assay based on transcriptomic analysis of Sarcoptes scabiei var. nyctereutis

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