Silver-doped graphene oxide nanocomposite triggers cytotoxicity and apoptosis in human hepatic normal and carcinoma cells

Daoud Ali, Saud Alarifi, Saad Alkahtani, Rafa S Almeer Department of Zoology, College of Science, King Saud University, Riyadh, Saudi Arabia Introduction: Graphene oxide nanoparticles have been widely used in industry and biomedical fields due to their unique physicochemical properties. However, c...

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Autores principales:	Ali D, Alarifi S, Alkahtani S, Almeer RS
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2018
Materias:	Oxidative stress CHANG and HepG2 cells Cytotoxicity DNA fragmentation Apoptosis Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/4b23b3bec2364078b16106743b547223
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spelling	oai:doaj.org-article:4b23b3bec2364078b16106743b5472232021-12-02T02:52:42ZSilver-doped graphene oxide nanocomposite triggers cytotoxicity and apoptosis in human hepatic normal and carcinoma cells1178-2013https://doaj.org/article/4b23b3bec2364078b16106743b5472232018-09-01T00:00:00Zhttps://www.dovepress.com/silver-doped-graphene-oxide-nanocomposite-triggers-cytotoxicity-and-ap-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Daoud Ali, Saud Alarifi, Saad Alkahtani, Rafa S Almeer Department of Zoology, College of Science, King Saud University, Riyadh, Saudi Arabia Introduction: Graphene oxide nanoparticles have been widely used in industry and biomedical fields due to their unique physicochemical properties. However, comparative cytotoxicity of silver-doped reduced graphene oxide (rGO–Ag) nanoparticles on normal and cancerous liver cells has not been well studied yet. Materials and methods: This study aimed at determining the toxic potential of rGO–Ag nanocomposite on human liver normal (CHANG) and cancer (HepG2) cells. The rGO–Ag nanocomposite was characterized by using different advanced instruments, namely, dynamic light scattering, scanning electron microscope, and transmission electron microscope. Results: The rGO–Ag nanocomposite reduced cell viability and impaired cell membrane integrity of CHANG and HepG2 cells in a dose-dependent manner. Additionally, it induced reactive oxygen species generation and reduced mitochondrial membrane potential in both cells in a dose-dependent manner. Moreover, the activity of oxidative enzymes such as lipid peroxide, superoxide dismutase, and catalase were increased and glutathione was reduced in both cells exposed to rGO–Ag nanocomposite. Pretreatment with N-acetylcysteine inhibited cytotoxicity and reactive oxygen species generation in CHANG and HepG2 cells exposed to rGO–Ag nanocomposite (50 µg/mL). DNA damage was determined by Comet assay and maximum DNA damage occurred at rGO–Ag nanocomposite (25 µg/mL) for 24 h. It is also valuable to inform that HepG2 cells appear to be slightly more susceptible to rGO–Ag nanocomposite exposure than CHANG cells. Conclusion: This result provides a basic comparative toxic effect of rGO–Ag nanocomposite on hepatic normal and cancerous liver cells. Keywords: oxidative stress, CHANG and HepG2 cells, cytotoxicity, DNA fragmentation, apoptosisAli DAlarifi SAlkahtani SAlmeer RSDove Medical PressarticleOxidative stressCHANG and HepG2 cellsCytotoxicityDNA fragmentationApoptosisMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 13, Pp 5685-5699 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Oxidative stress CHANG and HepG2 cells Cytotoxicity DNA fragmentation Apoptosis Medicine (General) R5-920
spellingShingle	Oxidative stress CHANG and HepG2 cells Cytotoxicity DNA fragmentation Apoptosis Medicine (General) R5-920 Ali D Alarifi S Alkahtani S Almeer RS Silver-doped graphene oxide nanocomposite triggers cytotoxicity and apoptosis in human hepatic normal and carcinoma cells
description	Daoud Ali, Saud Alarifi, Saad Alkahtani, Rafa S Almeer Department of Zoology, College of Science, King Saud University, Riyadh, Saudi Arabia Introduction: Graphene oxide nanoparticles have been widely used in industry and biomedical fields due to their unique physicochemical properties. However, comparative cytotoxicity of silver-doped reduced graphene oxide (rGO–Ag) nanoparticles on normal and cancerous liver cells has not been well studied yet. Materials and methods: This study aimed at determining the toxic potential of rGO–Ag nanocomposite on human liver normal (CHANG) and cancer (HepG2) cells. The rGO–Ag nanocomposite was characterized by using different advanced instruments, namely, dynamic light scattering, scanning electron microscope, and transmission electron microscope. Results: The rGO–Ag nanocomposite reduced cell viability and impaired cell membrane integrity of CHANG and HepG2 cells in a dose-dependent manner. Additionally, it induced reactive oxygen species generation and reduced mitochondrial membrane potential in both cells in a dose-dependent manner. Moreover, the activity of oxidative enzymes such as lipid peroxide, superoxide dismutase, and catalase were increased and glutathione was reduced in both cells exposed to rGO–Ag nanocomposite. Pretreatment with N-acetylcysteine inhibited cytotoxicity and reactive oxygen species generation in CHANG and HepG2 cells exposed to rGO–Ag nanocomposite (50 µg/mL). DNA damage was determined by Comet assay and maximum DNA damage occurred at rGO–Ag nanocomposite (25 µg/mL) for 24 h. It is also valuable to inform that HepG2 cells appear to be slightly more susceptible to rGO–Ag nanocomposite exposure than CHANG cells. Conclusion: This result provides a basic comparative toxic effect of rGO–Ag nanocomposite on hepatic normal and cancerous liver cells. Keywords: oxidative stress, CHANG and HepG2 cells, cytotoxicity, DNA fragmentation, apoptosis
format	article
author	Ali D Alarifi S Alkahtani S Almeer RS
author_facet	Ali D Alarifi S Alkahtani S Almeer RS
author_sort	Ali D
title	Silver-doped graphene oxide nanocomposite triggers cytotoxicity and apoptosis in human hepatic normal and carcinoma cells
title_short	Silver-doped graphene oxide nanocomposite triggers cytotoxicity and apoptosis in human hepatic normal and carcinoma cells
title_full	Silver-doped graphene oxide nanocomposite triggers cytotoxicity and apoptosis in human hepatic normal and carcinoma cells
title_fullStr	Silver-doped graphene oxide nanocomposite triggers cytotoxicity and apoptosis in human hepatic normal and carcinoma cells
title_full_unstemmed	Silver-doped graphene oxide nanocomposite triggers cytotoxicity and apoptosis in human hepatic normal and carcinoma cells
title_sort	silver-doped graphene oxide nanocomposite triggers cytotoxicity and apoptosis in human hepatic normal and carcinoma cells
publisher	Dove Medical Press
publishDate	2018
url	https://doaj.org/article/4b23b3bec2364078b16106743b547223
work_keys_str_mv	AT alid silverdopedgrapheneoxidenanocompositetriggerscytotoxicityandapoptosisinhumanhepaticnormalandcarcinomacells AT alarifis silverdopedgrapheneoxidenanocompositetriggerscytotoxicityandapoptosisinhumanhepaticnormalandcarcinomacells AT alkahtanis silverdopedgrapheneoxidenanocompositetriggerscytotoxicityandapoptosisinhumanhepaticnormalandcarcinomacells AT almeerrs silverdopedgrapheneoxidenanocompositetriggerscytotoxicityandapoptosisinhumanhepaticnormalandcarcinomacells
_version_	1718402099642892288

Silver-doped graphene oxide nanocomposite triggers cytotoxicity and apoptosis in human hepatic normal and carcinoma cells

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