Cohesin without cohesion: a novel role for Pds5 in Saccharomyces cerevisiae.

High fidelity chromosome segregation during mitosis requires that cells identify the products of DNA replication during S-phase and then maintain that identity until anaphase onset. Sister chromatid identity is achieved through cohesin complexes (Smc1, Smc3, and Mcd1 and Irr1/Scc3), but the structur...

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Autores principales:	Kevin Tong, Robert V Skibbens
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/4b2552c4fc24474ba2126773bcaf66b7
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spelling	oai:doaj.org-article:4b2552c4fc24474ba2126773bcaf66b72021-11-11T08:21:41ZCohesin without cohesion: a novel role for Pds5 in Saccharomyces cerevisiae.1932-620310.1371/journal.pone.0100470https://doaj.org/article/4b2552c4fc24474ba2126773bcaf66b72014-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24963665/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203High fidelity chromosome segregation during mitosis requires that cells identify the products of DNA replication during S-phase and then maintain that identity until anaphase onset. Sister chromatid identity is achieved through cohesin complexes (Smc1, Smc3, and Mcd1 and Irr1/Scc3), but the structure through which cohesins perform this task remains enigmatic. In the absence of unambiguous data, a popular model is that a subset of cohesin subunits form a huge ring-like structure that embraces both sister chromatids. This 'one-ring two-sister chromatid embrace' model makes clear predictions--including that premature cohesion loss in mitotic cells must occur through a substantial reduction in cohesin-DNA associations. We used chromatin immunoprecipitation to directly test for cohesin dissociation from well-established cohesin binding sites in mitotic cells inactivated for Pds5--a key cohesin regulatory protein. The results reveal little if any chromatin dissociation from cohesins, despite a regimen that produces both massive loss of sister chromatid tethering and cell inviability. We further excluded models that cohesion loss in mitotic cells inactivated for Pds5 arises through either cohesin subunit degradation, premature Hos1-dependent Smc3 de-acetylation or Rad61/WAPL-dependent regulation of cohesin dynamics. In combination, our findings support a model that cohesin complexes associate with each sister and that sister chromatid cohesion likely results from cohesin-cohesin interactions. We further assessed the role that Pds5 plays in cohesion establishment during S-phase. The results show that Pds5 inactivation can result in establishment defects despite normal cohesion loading and Smc3 acetylation, revealing a novel establishment role for Pds5 that is independent of these processes. The combination of findings provides important new insights that significantly impact current models of both cohesion establishment reactions and maintenance.Kevin TongRobert V SkibbensPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 6, p e100470 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Kevin Tong Robert V Skibbens Cohesin without cohesion: a novel role for Pds5 in Saccharomyces cerevisiae.
description	High fidelity chromosome segregation during mitosis requires that cells identify the products of DNA replication during S-phase and then maintain that identity until anaphase onset. Sister chromatid identity is achieved through cohesin complexes (Smc1, Smc3, and Mcd1 and Irr1/Scc3), but the structure through which cohesins perform this task remains enigmatic. In the absence of unambiguous data, a popular model is that a subset of cohesin subunits form a huge ring-like structure that embraces both sister chromatids. This 'one-ring two-sister chromatid embrace' model makes clear predictions--including that premature cohesion loss in mitotic cells must occur through a substantial reduction in cohesin-DNA associations. We used chromatin immunoprecipitation to directly test for cohesin dissociation from well-established cohesin binding sites in mitotic cells inactivated for Pds5--a key cohesin regulatory protein. The results reveal little if any chromatin dissociation from cohesins, despite a regimen that produces both massive loss of sister chromatid tethering and cell inviability. We further excluded models that cohesion loss in mitotic cells inactivated for Pds5 arises through either cohesin subunit degradation, premature Hos1-dependent Smc3 de-acetylation or Rad61/WAPL-dependent regulation of cohesin dynamics. In combination, our findings support a model that cohesin complexes associate with each sister and that sister chromatid cohesion likely results from cohesin-cohesin interactions. We further assessed the role that Pds5 plays in cohesion establishment during S-phase. The results show that Pds5 inactivation can result in establishment defects despite normal cohesion loading and Smc3 acetylation, revealing a novel establishment role for Pds5 that is independent of these processes. The combination of findings provides important new insights that significantly impact current models of both cohesion establishment reactions and maintenance.
format	article
author	Kevin Tong Robert V Skibbens
author_facet	Kevin Tong Robert V Skibbens
author_sort	Kevin Tong
title	Cohesin without cohesion: a novel role for Pds5 in Saccharomyces cerevisiae.
title_short	Cohesin without cohesion: a novel role for Pds5 in Saccharomyces cerevisiae.
title_full	Cohesin without cohesion: a novel role for Pds5 in Saccharomyces cerevisiae.
title_fullStr	Cohesin without cohesion: a novel role for Pds5 in Saccharomyces cerevisiae.
title_full_unstemmed	Cohesin without cohesion: a novel role for Pds5 in Saccharomyces cerevisiae.
title_sort	cohesin without cohesion: a novel role for pds5 in saccharomyces cerevisiae.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/4b2552c4fc24474ba2126773bcaf66b7
work_keys_str_mv	AT kevintong cohesinwithoutcohesionanovelroleforpds5insaccharomycescerevisiae AT robertvskibbens cohesinwithoutcohesionanovelroleforpds5insaccharomycescerevisiae
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Cohesin without cohesion: a novel role for Pds5 in Saccharomyces cerevisiae.

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