Structural basis for substrate gripping and translocation by the ClpB AAA+ disaggregase

Bacterial ClpB is a disaggregase that solubilizes protein aggregates. Here the authors present the 2.9 Å cryo-EM structure of a hyperactive variant of ClpB bound to the substrate casein in active translocation states and discuss its polypeptide translocation mechanism.

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Autores principales:	Alexandrea N. Rizo, JiaBei Lin, Stephanie N. Gates, Eric Tse, Stephen M. Bart, Laura M. Castellano, Frank DiMaio, James Shorter, Daniel R. Southworth
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2019
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/4b26b48e90114f5a86fdb8eed991c130
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spelling	oai:doaj.org-article:4b26b48e90114f5a86fdb8eed991c1302021-12-02T17:01:29ZStructural basis for substrate gripping and translocation by the ClpB AAA+ disaggregase10.1038/s41467-019-10150-y2041-1723https://doaj.org/article/4b26b48e90114f5a86fdb8eed991c1302019-06-01T00:00:00Zhttps://doi.org/10.1038/s41467-019-10150-yhttps://doaj.org/toc/2041-1723Bacterial ClpB is a disaggregase that solubilizes protein aggregates. Here the authors present the 2.9 Å cryo-EM structure of a hyperactive variant of ClpB bound to the substrate casein in active translocation states and discuss its polypeptide translocation mechanism.Alexandrea N. RizoJiaBei LinStephanie N. GatesEric TseStephen M. BartLaura M. CastellanoFrank DiMaioJames ShorterDaniel R. SouthworthNature PortfolioarticleScienceQENNature Communications, Vol 10, Iss 1, Pp 1-12 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Alexandrea N. Rizo JiaBei Lin Stephanie N. Gates Eric Tse Stephen M. Bart Laura M. Castellano Frank DiMaio James Shorter Daniel R. Southworth Structural basis for substrate gripping and translocation by the ClpB AAA+ disaggregase
description	Bacterial ClpB is a disaggregase that solubilizes protein aggregates. Here the authors present the 2.9 Å cryo-EM structure of a hyperactive variant of ClpB bound to the substrate casein in active translocation states and discuss its polypeptide translocation mechanism.
format	article
author	Alexandrea N. Rizo JiaBei Lin Stephanie N. Gates Eric Tse Stephen M. Bart Laura M. Castellano Frank DiMaio James Shorter Daniel R. Southworth
author_facet	Alexandrea N. Rizo JiaBei Lin Stephanie N. Gates Eric Tse Stephen M. Bart Laura M. Castellano Frank DiMaio James Shorter Daniel R. Southworth
author_sort	Alexandrea N. Rizo
title	Structural basis for substrate gripping and translocation by the ClpB AAA+ disaggregase
title_short	Structural basis for substrate gripping and translocation by the ClpB AAA+ disaggregase
title_full	Structural basis for substrate gripping and translocation by the ClpB AAA+ disaggregase
title_fullStr	Structural basis for substrate gripping and translocation by the ClpB AAA+ disaggregase
title_full_unstemmed	Structural basis for substrate gripping and translocation by the ClpB AAA+ disaggregase
title_sort	structural basis for substrate gripping and translocation by the clpb aaa+ disaggregase
publisher	Nature Portfolio
publishDate	2019
url	https://doaj.org/article/4b26b48e90114f5a86fdb8eed991c130
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Structural basis for substrate gripping and translocation by the ClpB AAA+ disaggregase

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