Integrating magnetic capabilities to intracellular chips for cell trapping

Abstract Current microtechnologies have shown plenty of room inside a living cell for silicon chips. Microchips as barcodes, biochemical sensors, mechanical sensors and even electrical devices have been internalized into living cells without interfering their cell viability. However, these technolog...

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Autores principales:	María Isabel Arjona, Consuelo González-Manchón, Sara Durán, Marta Duch, Rafael P. del Real, Abhinav Kadambi, Juan Pablo Agusil, Mariano Redondo-Horcajo, Lluïsa Pérez-García, Elvira Gómez, Teresa Suárez, José Antonio Plaza
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/4b2cf76c152545d19be07390868348a7
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spelling	oai:doaj.org-article:4b2cf76c152545d19be07390868348a72021-12-02T18:02:15ZIntegrating magnetic capabilities to intracellular chips for cell trapping10.1038/s41598-021-98095-52045-2322https://doaj.org/article/4b2cf76c152545d19be07390868348a72021-09-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-98095-5https://doaj.org/toc/2045-2322Abstract Current microtechnologies have shown plenty of room inside a living cell for silicon chips. Microchips as barcodes, biochemical sensors, mechanical sensors and even electrical devices have been internalized into living cells without interfering their cell viability. However, these technologies lack from the ability to trap and preconcentrate cells in a specific region, which are prerequisites for cell separation, purification and posterior studies with enhanced sensitivity. Magnetic manipulation of microobjects, which allows a non-contacting method, has become an attractive and promising technique at small scales. Here, we show intracellular Ni-based chips with magnetic capabilities to allow cell enrichment. As a proof of concept of the potential to integrate multiple functionalities on a single device of this technique, we combine coding and magnetic manipulation capabilities in a single device. Devices were found to be internalized by HeLa cells without interfering in their viability. We demonstrated the tagging of a subpopulation of cells and their subsequent magnetic trapping with internalized barcodes subjected to a force up to 2.57 pN (for magnet-cells distance of 4.9 mm). The work opens the venue for future intracellular chips that integrate multiple functionalities with the magnetic manipulation of cells.María Isabel ArjonaConsuelo González-ManchónSara DuránMarta DuchRafael P. del RealAbhinav KadambiJuan Pablo AgusilMariano Redondo-HorcajoLluïsa Pérez-GarcíaElvira GómezTeresa SuárezJosé Antonio PlazaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-9 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q María Isabel Arjona Consuelo González-Manchón Sara Durán Marta Duch Rafael P. del Real Abhinav Kadambi Juan Pablo Agusil Mariano Redondo-Horcajo Lluïsa Pérez-García Elvira Gómez Teresa Suárez José Antonio Plaza Integrating magnetic capabilities to intracellular chips for cell trapping
description	Abstract Current microtechnologies have shown plenty of room inside a living cell for silicon chips. Microchips as barcodes, biochemical sensors, mechanical sensors and even electrical devices have been internalized into living cells without interfering their cell viability. However, these technologies lack from the ability to trap and preconcentrate cells in a specific region, which are prerequisites for cell separation, purification and posterior studies with enhanced sensitivity. Magnetic manipulation of microobjects, which allows a non-contacting method, has become an attractive and promising technique at small scales. Here, we show intracellular Ni-based chips with magnetic capabilities to allow cell enrichment. As a proof of concept of the potential to integrate multiple functionalities on a single device of this technique, we combine coding and magnetic manipulation capabilities in a single device. Devices were found to be internalized by HeLa cells without interfering in their viability. We demonstrated the tagging of a subpopulation of cells and their subsequent magnetic trapping with internalized barcodes subjected to a force up to 2.57 pN (for magnet-cells distance of 4.9 mm). The work opens the venue for future intracellular chips that integrate multiple functionalities with the magnetic manipulation of cells.
format	article
author	María Isabel Arjona Consuelo González-Manchón Sara Durán Marta Duch Rafael P. del Real Abhinav Kadambi Juan Pablo Agusil Mariano Redondo-Horcajo Lluïsa Pérez-García Elvira Gómez Teresa Suárez José Antonio Plaza
author_facet	María Isabel Arjona Consuelo González-Manchón Sara Durán Marta Duch Rafael P. del Real Abhinav Kadambi Juan Pablo Agusil Mariano Redondo-Horcajo Lluïsa Pérez-García Elvira Gómez Teresa Suárez José Antonio Plaza
author_sort	María Isabel Arjona
title	Integrating magnetic capabilities to intracellular chips for cell trapping
title_short	Integrating magnetic capabilities to intracellular chips for cell trapping
title_full	Integrating magnetic capabilities to intracellular chips for cell trapping
title_fullStr	Integrating magnetic capabilities to intracellular chips for cell trapping
title_full_unstemmed	Integrating magnetic capabilities to intracellular chips for cell trapping
title_sort	integrating magnetic capabilities to intracellular chips for cell trapping
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/4b2cf76c152545d19be07390868348a7
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Integrating magnetic capabilities to intracellular chips for cell trapping

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