Impaired sense of smell in a Drosophila Parkinson's model.

Parkinson's disease (PD) is one of the most common neurodegenerative disease characterized by the clinical triad: tremor, akinesia and rigidity. Several studies have suggested that PD patients show disturbances in olfaction at the earliest onset of the disease. The fruit fly Drosophila melanoga...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Simone Poddighe, Krishna Moorthi Bhat, Maria Dolores Setzu, Paolo Solla, Anna Maria Angioy, Roberto Marotta, Roberta Ruffilli, Francesco Marrosu, Anna Liscia
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/4b37327219524f50b4b5a2207a92cba0
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:4b37327219524f50b4b5a2207a92cba0
record_format	dspace
spelling	oai:doaj.org-article:4b37327219524f50b4b5a2207a92cba02021-11-18T08:57:42ZImpaired sense of smell in a Drosophila Parkinson's model.1932-620310.1371/journal.pone.0073156https://doaj.org/article/4b37327219524f50b4b5a2207a92cba02013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24009736/?tool=EBIhttps://doaj.org/toc/1932-6203Parkinson's disease (PD) is one of the most common neurodegenerative disease characterized by the clinical triad: tremor, akinesia and rigidity. Several studies have suggested that PD patients show disturbances in olfaction at the earliest onset of the disease. The fruit fly Drosophila melanogaster is becoming a powerful model organism to study neurodegenerative diseases. We sought to use this system to explore olfactory dysfunction, if any, in PINK1 mutants, which is a model for PD. PINK1 mutants display many important diagnostic symptoms of the disease such as akinetic motor behavior. In the present study, we describe for the first time, to the best of our knowledge, neurophysiological and neuroanatomical results concerning the olfactory function in PINK1 mutant flies. Electroantennograms were recorded in response to synthetic and natural volatiles (essential oils) from groups of PINK1 mutant adults at three different time points in their life cycle: one from 3-5 day-old flies, from 15-20 and from 27-30 days. The results obtained were compared with the same age-groups of wild type flies. We found that mutant adults showed a decrease in the olfactory response to 1-hexanol, α-pinene and essential oil volatiles. This olfactory response in mutant adults decreased even more as the flies aged. Immunohistological analysis of the antennal lobes in these mutants revealed structural abnormalities, especially in the expression of Bruchpilot protein, a marker for synaptic active zones. The combination of electrophysiological and morphological results suggests that the altered synaptic organization may be due to a neurodegenerative process. Our results indicate that this model can be used as a tool for understanding PD pathogensis and pathophysiology. These results help to explore the potential of using olfaction as a means of monitoring PD progression and developing new treatments.Simone PoddigheKrishna Moorthi BhatMaria Dolores SetzuPaolo SollaAnna Maria AngioyRoberto MarottaRoberta RuffilliFrancesco MarrosuAnna LisciaPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 8, p e73156 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Simone Poddighe Krishna Moorthi Bhat Maria Dolores Setzu Paolo Solla Anna Maria Angioy Roberto Marotta Roberta Ruffilli Francesco Marrosu Anna Liscia Impaired sense of smell in a Drosophila Parkinson's model.
description	Parkinson's disease (PD) is one of the most common neurodegenerative disease characterized by the clinical triad: tremor, akinesia and rigidity. Several studies have suggested that PD patients show disturbances in olfaction at the earliest onset of the disease. The fruit fly Drosophila melanogaster is becoming a powerful model organism to study neurodegenerative diseases. We sought to use this system to explore olfactory dysfunction, if any, in PINK1 mutants, which is a model for PD. PINK1 mutants display many important diagnostic symptoms of the disease such as akinetic motor behavior. In the present study, we describe for the first time, to the best of our knowledge, neurophysiological and neuroanatomical results concerning the olfactory function in PINK1 mutant flies. Electroantennograms were recorded in response to synthetic and natural volatiles (essential oils) from groups of PINK1 mutant adults at three different time points in their life cycle: one from 3-5 day-old flies, from 15-20 and from 27-30 days. The results obtained were compared with the same age-groups of wild type flies. We found that mutant adults showed a decrease in the olfactory response to 1-hexanol, α-pinene and essential oil volatiles. This olfactory response in mutant adults decreased even more as the flies aged. Immunohistological analysis of the antennal lobes in these mutants revealed structural abnormalities, especially in the expression of Bruchpilot protein, a marker for synaptic active zones. The combination of electrophysiological and morphological results suggests that the altered synaptic organization may be due to a neurodegenerative process. Our results indicate that this model can be used as a tool for understanding PD pathogensis and pathophysiology. These results help to explore the potential of using olfaction as a means of monitoring PD progression and developing new treatments.
format	article
author	Simone Poddighe Krishna Moorthi Bhat Maria Dolores Setzu Paolo Solla Anna Maria Angioy Roberto Marotta Roberta Ruffilli Francesco Marrosu Anna Liscia
author_facet	Simone Poddighe Krishna Moorthi Bhat Maria Dolores Setzu Paolo Solla Anna Maria Angioy Roberto Marotta Roberta Ruffilli Francesco Marrosu Anna Liscia
author_sort	Simone Poddighe
title	Impaired sense of smell in a Drosophila Parkinson's model.
title_short	Impaired sense of smell in a Drosophila Parkinson's model.
title_full	Impaired sense of smell in a Drosophila Parkinson's model.
title_fullStr	Impaired sense of smell in a Drosophila Parkinson's model.
title_full_unstemmed	Impaired sense of smell in a Drosophila Parkinson's model.
title_sort	impaired sense of smell in a drosophila parkinson's model.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/4b37327219524f50b4b5a2207a92cba0
work_keys_str_mv	AT simonepoddighe impairedsenseofsmellinadrosophilaparkinsonsmodel AT krishnamoorthibhat impairedsenseofsmellinadrosophilaparkinsonsmodel AT mariadoloressetzu impairedsenseofsmellinadrosophilaparkinsonsmodel AT paolosolla impairedsenseofsmellinadrosophilaparkinsonsmodel AT annamariaangioy impairedsenseofsmellinadrosophilaparkinsonsmodel AT robertomarotta impairedsenseofsmellinadrosophilaparkinsonsmodel AT robertaruffilli impairedsenseofsmellinadrosophilaparkinsonsmodel AT francescomarrosu impairedsenseofsmellinadrosophilaparkinsonsmodel AT annaliscia impairedsenseofsmellinadrosophilaparkinsonsmodel
_version_	1718421079745101824

Impaired sense of smell in a Drosophila Parkinson's model.

Ejemplares similares