Olig2-astrocytes express neutral amino acid transporter SLC7A10 (Asc-1) in the adult brain

Abstract We have reported that the transcription factor Olig2 labels a subpopulation of astrocytes (Olig2-astrocytes), which show distribution patterns different from those of GFAP-expressing astrocytes (GFAP-astrocytes) in the adult brain. Here, to uncover the specific functions of Olig2-astrocytes...

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Autores principales:	Kouko Tatsumi, Kaoru Kinugawa, Ayami Isonishi, Masahiro Kitabatake, Hiroaki Okuda, Shoko Takemura, Tatsuhide Tanaka, Eiichiro Mori, Akio Wanaka
Formato:	article
Lenguaje:	EN
Publicado:	BMC 2021
Materias:	Neurology. Diseases of the nervous system RC346-429
Acceso en línea:	https://doaj.org/article/4b3a053f7aa449d599730651a3f9bab9
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spelling	oai:doaj.org-article:4b3a053f7aa449d599730651a3f9bab92021-11-14T12:42:41ZOlig2-astrocytes express neutral amino acid transporter SLC7A10 (Asc-1) in the adult brain10.1186/s13041-021-00874-81756-6606https://doaj.org/article/4b3a053f7aa449d599730651a3f9bab92021-11-01T00:00:00Zhttps://doi.org/10.1186/s13041-021-00874-8https://doaj.org/toc/1756-6606Abstract We have reported that the transcription factor Olig2 labels a subpopulation of astrocytes (Olig2-astrocytes), which show distribution patterns different from those of GFAP-expressing astrocytes (GFAP-astrocytes) in the adult brain. Here, to uncover the specific functions of Olig2-astrocytes, we first analyzed public single-cell RNA-seq databases of adult mouse brains. Unbiased classification of gene expression profiles and subsequent gene ontology analyses revealed that the majority of Olig2-astrocytes belonged to an astrocytic cluster that is enriched for transporter-related genes. SLC7A10 (also known as ASC-1) was one of the representative neutral amino acid transporter genes in the cluster. To complement the in silico data analyses, we differentially isolated Olig2- and GFAP-astrocytes from the same frozen section of the lateral globus pallidus using laser microdissection and compared their gene expression by quantitative reverse transcription PCR. We confirmed that Olig2 and GFAP mRNAs were preferentially expressed in the Olig2- and GFAP-astrocytes, respectively, indicating that the laser microdissection method yielded minimal cross-contamination between two types of cells. The Olig2-astrocytes expressed significantly higher levels of SLC7A10 mRNA than the GFAP-astrocytes, corroborating the in silico data. We next localized SLC7A10 protein by immunohistochemistry in the lateral globus pallidus, which was also genetically labeled for Olig2. SLC7A10 co-localized with Olig2-genetic labeling, especially on the fine processes of Olig2-astrocytes. These results are consistent with the recent discovery that SLC7A10 is expressed not only in neurons but also in a subset of astrocytes. Taken together, our findings suggest that SLC7A10 exerts specific functions in Olig2-astrocytes of the adult brain.Kouko TatsumiKaoru KinugawaAyami IsonishiMasahiro KitabatakeHiroaki OkudaShoko TakemuraTatsuhide TanakaEiichiro MoriAkio WanakaBMCarticleNeurology. Diseases of the nervous systemRC346-429ENMolecular Brain, Vol 14, Iss 1, Pp 1-12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Neurology. Diseases of the nervous system RC346-429
spellingShingle	Neurology. Diseases of the nervous system RC346-429 Kouko Tatsumi Kaoru Kinugawa Ayami Isonishi Masahiro Kitabatake Hiroaki Okuda Shoko Takemura Tatsuhide Tanaka Eiichiro Mori Akio Wanaka Olig2-astrocytes express neutral amino acid transporter SLC7A10 (Asc-1) in the adult brain
description	Abstract We have reported that the transcription factor Olig2 labels a subpopulation of astrocytes (Olig2-astrocytes), which show distribution patterns different from those of GFAP-expressing astrocytes (GFAP-astrocytes) in the adult brain. Here, to uncover the specific functions of Olig2-astrocytes, we first analyzed public single-cell RNA-seq databases of adult mouse brains. Unbiased classification of gene expression profiles and subsequent gene ontology analyses revealed that the majority of Olig2-astrocytes belonged to an astrocytic cluster that is enriched for transporter-related genes. SLC7A10 (also known as ASC-1) was one of the representative neutral amino acid transporter genes in the cluster. To complement the in silico data analyses, we differentially isolated Olig2- and GFAP-astrocytes from the same frozen section of the lateral globus pallidus using laser microdissection and compared their gene expression by quantitative reverse transcription PCR. We confirmed that Olig2 and GFAP mRNAs were preferentially expressed in the Olig2- and GFAP-astrocytes, respectively, indicating that the laser microdissection method yielded minimal cross-contamination between two types of cells. The Olig2-astrocytes expressed significantly higher levels of SLC7A10 mRNA than the GFAP-astrocytes, corroborating the in silico data. We next localized SLC7A10 protein by immunohistochemistry in the lateral globus pallidus, which was also genetically labeled for Olig2. SLC7A10 co-localized with Olig2-genetic labeling, especially on the fine processes of Olig2-astrocytes. These results are consistent with the recent discovery that SLC7A10 is expressed not only in neurons but also in a subset of astrocytes. Taken together, our findings suggest that SLC7A10 exerts specific functions in Olig2-astrocytes of the adult brain.
format	article
author	Kouko Tatsumi Kaoru Kinugawa Ayami Isonishi Masahiro Kitabatake Hiroaki Okuda Shoko Takemura Tatsuhide Tanaka Eiichiro Mori Akio Wanaka
author_facet	Kouko Tatsumi Kaoru Kinugawa Ayami Isonishi Masahiro Kitabatake Hiroaki Okuda Shoko Takemura Tatsuhide Tanaka Eiichiro Mori Akio Wanaka
author_sort	Kouko Tatsumi
title	Olig2-astrocytes express neutral amino acid transporter SLC7A10 (Asc-1) in the adult brain
title_short	Olig2-astrocytes express neutral amino acid transporter SLC7A10 (Asc-1) in the adult brain
title_full	Olig2-astrocytes express neutral amino acid transporter SLC7A10 (Asc-1) in the adult brain
title_fullStr	Olig2-astrocytes express neutral amino acid transporter SLC7A10 (Asc-1) in the adult brain
title_full_unstemmed	Olig2-astrocytes express neutral amino acid transporter SLC7A10 (Asc-1) in the adult brain
title_sort	olig2-astrocytes express neutral amino acid transporter slc7a10 (asc-1) in the adult brain
publisher	BMC
publishDate	2021
url	https://doaj.org/article/4b3a053f7aa449d599730651a3f9bab9
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Olig2-astrocytes express neutral amino acid transporter SLC7A10 (Asc-1) in the adult brain

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