Transcription reprogramming during root nodule development in Medicago truncatula.

Many genes which are associated with root nodule development and activity in the model legume Medicago truncatula have been described. However information on precise stages of activation of these genes and their corresponding transcriptional regulators is often lacking. Whether these regulators are...

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Autores principales:	Sandra Moreau, Marion Verdenaud, Thomas Ott, Sébastien Letort, Françoise de Billy, Andreas Niebel, Jérôme Gouzy, Fernanda de Carvalho-Niebel, Pascal Gamas
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2011
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/4b3e84475bc644c1a3f18d0ef3a8344e
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spelling	oai:doaj.org-article:4b3e84475bc644c1a3f18d0ef3a8344e2021-11-18T06:59:43ZTranscription reprogramming during root nodule development in Medicago truncatula.1932-620310.1371/journal.pone.0016463https://doaj.org/article/4b3e84475bc644c1a3f18d0ef3a8344e2011-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/21304580/?tool=EBIhttps://doaj.org/toc/1932-6203Many genes which are associated with root nodule development and activity in the model legume Medicago truncatula have been described. However information on precise stages of activation of these genes and their corresponding transcriptional regulators is often lacking. Whether these regulators are shared with other plant developmental programs also remains an open question. Here detailed microarray analyses have been used to study the transcriptome of root nodules induced by either wild type or mutant strains of Sinorhizobium meliloti. In this way we have defined eight major activation patterns in nodules and identified associated potential regulatory genes. We have shown that transcription reprogramming during consecutive stages of nodule differentiation occurs in four major phases, respectively associated with (i) early signalling events and/or bacterial infection; plant cell differentiation that is either (ii) independent or (iii) dependent on bacteroid differentiation; (iv) nitrogen fixation. Differential expression of several genes involved in cytokinin biosynthesis was observed in early symbiotic nodule zones, suggesting that cytokinin levels are actively controlled in this region. Taking advantage of databases recently developed for M. truncatula, we identified a small subset of gene expression regulators that were exclusively or predominantly expressed in nodules, whereas most other regulators were also activated under other conditions, and notably in response to abiotic or biotic stresses. We found evidence suggesting the activation of the jasmonate pathway in both wild type and mutant nodules, thus raising questions about the role of jasmonate during nodule development. Finally, quantitative RT-PCR was used to analyse the expression of a series of nodule regulator and marker genes at early symbiotic stages in roots and allowed us to distinguish several early stages of gene expression activation or repression.Sandra MoreauMarion VerdenaudThomas OttSébastien LetortFrançoise de BillyAndreas NiebelJérôme GouzyFernanda de Carvalho-NiebelPascal GamasPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 6, Iss 1, p e16463 (2011)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Sandra Moreau Marion Verdenaud Thomas Ott Sébastien Letort Françoise de Billy Andreas Niebel Jérôme Gouzy Fernanda de Carvalho-Niebel Pascal Gamas Transcription reprogramming during root nodule development in Medicago truncatula.
description	Many genes which are associated with root nodule development and activity in the model legume Medicago truncatula have been described. However information on precise stages of activation of these genes and their corresponding transcriptional regulators is often lacking. Whether these regulators are shared with other plant developmental programs also remains an open question. Here detailed microarray analyses have been used to study the transcriptome of root nodules induced by either wild type or mutant strains of Sinorhizobium meliloti. In this way we have defined eight major activation patterns in nodules and identified associated potential regulatory genes. We have shown that transcription reprogramming during consecutive stages of nodule differentiation occurs in four major phases, respectively associated with (i) early signalling events and/or bacterial infection; plant cell differentiation that is either (ii) independent or (iii) dependent on bacteroid differentiation; (iv) nitrogen fixation. Differential expression of several genes involved in cytokinin biosynthesis was observed in early symbiotic nodule zones, suggesting that cytokinin levels are actively controlled in this region. Taking advantage of databases recently developed for M. truncatula, we identified a small subset of gene expression regulators that were exclusively or predominantly expressed in nodules, whereas most other regulators were also activated under other conditions, and notably in response to abiotic or biotic stresses. We found evidence suggesting the activation of the jasmonate pathway in both wild type and mutant nodules, thus raising questions about the role of jasmonate during nodule development. Finally, quantitative RT-PCR was used to analyse the expression of a series of nodule regulator and marker genes at early symbiotic stages in roots and allowed us to distinguish several early stages of gene expression activation or repression.
format	article
author	Sandra Moreau Marion Verdenaud Thomas Ott Sébastien Letort Françoise de Billy Andreas Niebel Jérôme Gouzy Fernanda de Carvalho-Niebel Pascal Gamas
author_facet	Sandra Moreau Marion Verdenaud Thomas Ott Sébastien Letort Françoise de Billy Andreas Niebel Jérôme Gouzy Fernanda de Carvalho-Niebel Pascal Gamas
author_sort	Sandra Moreau
title	Transcription reprogramming during root nodule development in Medicago truncatula.
title_short	Transcription reprogramming during root nodule development in Medicago truncatula.
title_full	Transcription reprogramming during root nodule development in Medicago truncatula.
title_fullStr	Transcription reprogramming during root nodule development in Medicago truncatula.
title_full_unstemmed	Transcription reprogramming during root nodule development in Medicago truncatula.
title_sort	transcription reprogramming during root nodule development in medicago truncatula.
publisher	Public Library of Science (PLoS)
publishDate	2011
url	https://doaj.org/article/4b3e84475bc644c1a3f18d0ef3a8344e
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Transcription reprogramming during root nodule development in Medicago truncatula.

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