Antiviral proteinase inhibitors of plant and animal origin

Introduction: Over the past 10 years, much attention has been paid to the development of new antiviral drugs based on the suppression of the proteolytic activity of enzymes by trypsin inhibitors of plant and animal origin. Material and methods: We used a trypsin inhibitor from barley, trielin- (iso...

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Autores principales:	Valentina Divocha, Irina Komarevzeva
Formato:	article
Lenguaje:	EN
Publicado:	Emergency Department of Hospital San Pedro (Logroño, Spain) 2020
Materias:	influenza virus tripsin-like proteinase inhibitor Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/4b4243cfe2c2496e8421192ec8fdd75e
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spelling	oai:doaj.org-article:4b4243cfe2c2496e8421192ec8fdd75e2021-12-02T17:45:29ZAntiviral proteinase inhibitors of plant and animal origin10.5281/zenodo.37017282695-5075https://doaj.org/article/4b4243cfe2c2496e8421192ec8fdd75e2020-03-01T00:00:00Zhttps://doi.org/10.5281/zenodo.3701728https://doaj.org/toc/2695-5075Introduction: Over the past 10 years, much attention has been paid to the development of new antiviral drugs based on the suppression of the proteolytic activity of enzymes by trypsin inhibitors of plant and animal origin. Material and methods: We used a trypsin inhibitor from barley, trielin- (isolated by employees of the Agro-Industrial Institute of Selection and Genetics of the Ukrainian Academy of Sciences from the salivary glands of a dog); ovomukoid (isolated from duck eggs by employees of N, I, Bach Research Institute of Biology, Russian Academy of Sciences); Influenza virus APR 8/34 (fourth passage), adapted to the lungs of mice at a dose of 20 LD /0.1 ml, titre HA( hemagglutenin) 1:32) ,white BALB/c mice weighing 12-14 g. Infection with influenza virus and treatment with inhibitors was carried out intranasally under light ether anesthesia. Doses studied were: 0.5mg/ml; 2.5 mg/ml; 5.0 mg/ml; The treatment regimen of 10 mg/ml differed only in the initial stages (1 hour before infection, during infection and 1 hour after infection, and then 6 hours after infection, 24 hours after infection, 48 hours after infection, 72 hours after infection and 96 hours after infection). Results and discussion: We found that an in vivo inhibitor from barley at a dose of 10 g/l delayed the development of influenza for 8 days. The ovomukoid possessed only prophylactic properties at a dose of 100 gamma / ml. With an increase in dose, it was toxic to animals. Trielin at a dose of 10 g/l had a pronounced therapeutic effect in influenza and was not toxic. The presence of hemagglutinin influenza virus in the lungs of treated mice was observed only on the 10th day after infection; 40% of the animals remained alive for 14 days (observation period).Valentina DivochaIrina KomarevzevaEmergency Department of Hospital San Pedro (Logroño, Spain)articleinfluenza virustripsin-like proteinaseinhibitorMedicine (General)R5-920ENIberoamerican Journal of Medicine, Vol 2, Iss 2, Pp 43-48 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	influenza virus tripsin-like proteinase inhibitor Medicine (General) R5-920
spellingShingle	influenza virus tripsin-like proteinase inhibitor Medicine (General) R5-920 Valentina Divocha Irina Komarevzeva Antiviral proteinase inhibitors of plant and animal origin
description	Introduction: Over the past 10 years, much attention has been paid to the development of new antiviral drugs based on the suppression of the proteolytic activity of enzymes by trypsin inhibitors of plant and animal origin. Material and methods: We used a trypsin inhibitor from barley, trielin- (isolated by employees of the Agro-Industrial Institute of Selection and Genetics of the Ukrainian Academy of Sciences from the salivary glands of a dog); ovomukoid (isolated from duck eggs by employees of N, I, Bach Research Institute of Biology, Russian Academy of Sciences); Influenza virus APR 8/34 (fourth passage), adapted to the lungs of mice at a dose of 20 LD /0.1 ml, titre HA( hemagglutenin) 1:32) ,white BALB/c mice weighing 12-14 g. Infection with influenza virus and treatment with inhibitors was carried out intranasally under light ether anesthesia. Doses studied were: 0.5mg/ml; 2.5 mg/ml; 5.0 mg/ml; The treatment regimen of 10 mg/ml differed only in the initial stages (1 hour before infection, during infection and 1 hour after infection, and then 6 hours after infection, 24 hours after infection, 48 hours after infection, 72 hours after infection and 96 hours after infection). Results and discussion: We found that an in vivo inhibitor from barley at a dose of 10 g/l delayed the development of influenza for 8 days. The ovomukoid possessed only prophylactic properties at a dose of 100 gamma / ml. With an increase in dose, it was toxic to animals. Trielin at a dose of 10 g/l had a pronounced therapeutic effect in influenza and was not toxic. The presence of hemagglutinin influenza virus in the lungs of treated mice was observed only on the 10th day after infection; 40% of the animals remained alive for 14 days (observation period).
format	article
author	Valentina Divocha Irina Komarevzeva
author_facet	Valentina Divocha Irina Komarevzeva
author_sort	Valentina Divocha
title	Antiviral proteinase inhibitors of plant and animal origin
title_short	Antiviral proteinase inhibitors of plant and animal origin
title_full	Antiviral proteinase inhibitors of plant and animal origin
title_fullStr	Antiviral proteinase inhibitors of plant and animal origin
title_full_unstemmed	Antiviral proteinase inhibitors of plant and animal origin
title_sort	antiviral proteinase inhibitors of plant and animal origin
publisher	Emergency Department of Hospital San Pedro (Logroño, Spain)
publishDate	2020
url	https://doaj.org/article/4b4243cfe2c2496e8421192ec8fdd75e
work_keys_str_mv	AT valentinadivocha antiviralproteinaseinhibitorsofplantandanimalorigin AT irinakomarevzeva antiviralproteinaseinhibitorsofplantandanimalorigin
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Antiviral proteinase inhibitors of plant and animal origin

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