Effect of Cannabis Smoke Condensate on <i>C. albicans</i> Growth and Biofilm Formation
The most common use of cannabis is smoking. The oral ecosystem, among other constituents, can be deregulated by the presence of cannabis smoke in the oral cavity. We evaluated the effect of cannabis smoke condensate (CSC) on the behavior of <i>Candida albicans</i>, a common yeast found i...
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2021
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oai:doaj.org-article:4b4252b1b9134f91aca4cad3e429075e2021-11-25T18:25:20ZEffect of Cannabis Smoke Condensate on <i>C. albicans</i> Growth and Biofilm Formation10.3390/microorganisms91123482076-2607https://doaj.org/article/4b4252b1b9134f91aca4cad3e429075e2021-11-01T00:00:00Zhttps://www.mdpi.com/2076-2607/9/11/2348https://doaj.org/toc/2076-2607The most common use of cannabis is smoking. The oral ecosystem, among other constituents, can be deregulated by the presence of cannabis smoke in the oral cavity. We evaluated the effect of cannabis smoke condensate (CSC) on the behavior of <i>Candida albicans</i>, a common yeast found in the oral cavity. The yeast was first cultured with different concentrations of CSC, and its growth was evaluated. The transition from the blastospore to the hyphal form and the hyphae size were assessed after 3 and 6 h, along with biofilm formation after 72 h of contact with CSC. The response of <i>C. albicans</i> to oxidative (H<sub>2</sub>O<sub>2</sub>) stress was also examined. Our results show that CSC contained high amounts of THC (about 1055 ppm), CBN (63 ppm), and CBG (about 47 ppm). The presence of various concentrations of CSC in the culture medium increased <i>C. albicans</i> growth. CSC also contributed to increases in both the hyphal length and biofilm mass. Following oxidative stress (H<sub>2</sub>O<sub>2</sub> at either 100 or 500 μM), CSC prevented the damaging effect of H<sub>2</sub>O<sub>2</sub> on both <i>C. albicans</i> shape and growth. These findings support clinical observations demonstrating that cannabis may promote <i>C.</i> <i>albicans</i> growth and oral candidiasis.Neftaha TaziXavier PigeonJérôme Mulamba Mbuyi-BoisvertSimon GiretFrançois BélandMahmoud RouabhiaMDPI AGarticlecannabis<i>C. albicans</i>growthbiofilmsoxidative stressBiology (General)QH301-705.5ENMicroorganisms, Vol 9, Iss 2348, p 2348 (2021) |
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DOAJ |
| collection |
DOAJ |
| language |
EN |
| topic |
cannabis <i>C. albicans</i> growth biofilms oxidative stress Biology (General) QH301-705.5 |
| spellingShingle |
cannabis <i>C. albicans</i> growth biofilms oxidative stress Biology (General) QH301-705.5 Neftaha Tazi Xavier Pigeon Jérôme Mulamba Mbuyi-Boisvert Simon Giret François Béland Mahmoud Rouabhia Effect of Cannabis Smoke Condensate on <i>C. albicans</i> Growth and Biofilm Formation |
| description |
The most common use of cannabis is smoking. The oral ecosystem, among other constituents, can be deregulated by the presence of cannabis smoke in the oral cavity. We evaluated the effect of cannabis smoke condensate (CSC) on the behavior of <i>Candida albicans</i>, a common yeast found in the oral cavity. The yeast was first cultured with different concentrations of CSC, and its growth was evaluated. The transition from the blastospore to the hyphal form and the hyphae size were assessed after 3 and 6 h, along with biofilm formation after 72 h of contact with CSC. The response of <i>C. albicans</i> to oxidative (H<sub>2</sub>O<sub>2</sub>) stress was also examined. Our results show that CSC contained high amounts of THC (about 1055 ppm), CBN (63 ppm), and CBG (about 47 ppm). The presence of various concentrations of CSC in the culture medium increased <i>C. albicans</i> growth. CSC also contributed to increases in both the hyphal length and biofilm mass. Following oxidative stress (H<sub>2</sub>O<sub>2</sub> at either 100 or 500 μM), CSC prevented the damaging effect of H<sub>2</sub>O<sub>2</sub> on both <i>C. albicans</i> shape and growth. These findings support clinical observations demonstrating that cannabis may promote <i>C.</i> <i>albicans</i> growth and oral candidiasis. |
| format |
article |
| author |
Neftaha Tazi Xavier Pigeon Jérôme Mulamba Mbuyi-Boisvert Simon Giret François Béland Mahmoud Rouabhia |
| author_facet |
Neftaha Tazi Xavier Pigeon Jérôme Mulamba Mbuyi-Boisvert Simon Giret François Béland Mahmoud Rouabhia |
| author_sort |
Neftaha Tazi |
| title |
Effect of Cannabis Smoke Condensate on <i>C. albicans</i> Growth and Biofilm Formation |
| title_short |
Effect of Cannabis Smoke Condensate on <i>C. albicans</i> Growth and Biofilm Formation |
| title_full |
Effect of Cannabis Smoke Condensate on <i>C. albicans</i> Growth and Biofilm Formation |
| title_fullStr |
Effect of Cannabis Smoke Condensate on <i>C. albicans</i> Growth and Biofilm Formation |
| title_full_unstemmed |
Effect of Cannabis Smoke Condensate on <i>C. albicans</i> Growth and Biofilm Formation |
| title_sort |
effect of cannabis smoke condensate on <i>c. albicans</i> growth and biofilm formation |
| publisher |
MDPI AG |
| publishDate |
2021 |
| url |
https://doaj.org/article/4b4252b1b9134f91aca4cad3e429075e |
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AT neftahatazi effectofcannabissmokecondensateonicalbicansigrowthandbiofilmformation AT xavierpigeon effectofcannabissmokecondensateonicalbicansigrowthandbiofilmformation AT jeromemulambambuyiboisvert effectofcannabissmokecondensateonicalbicansigrowthandbiofilmformation AT simongiret effectofcannabissmokecondensateonicalbicansigrowthandbiofilmformation AT francoisbeland effectofcannabissmokecondensateonicalbicansigrowthandbiofilmformation AT mahmoudrouabhia effectofcannabissmokecondensateonicalbicansigrowthandbiofilmformation |
| _version_ |
1718411192229167104 |