LncRNA PVT1 promotes cervical cancer progression by sponging miR-503 to upregulate ARL2 expression

Cervical cancer (CC) is a huge threat to the health of women worldwide. Long non-coding RNA plasmacytoma variant translocation 1 gene (PVT1) was proved to be associated with the development of diverse human cancers, including CC. Nevertheless, the exact mechanism of PVT1 in CC progression remains un...

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Autores principales:	Liu Weiwei, Yao Dongmei, Huang Bo
Formato:	article
Lenguaje:	EN
Publicado:	De Gruyter 2021
Materias:	lncrna pvt1 mir-503 arl2 cervical cancer progression Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/4b435add956d45e59deb6153dfc15a41
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spelling	oai:doaj.org-article:4b435add956d45e59deb6153dfc15a412021-12-05T14:10:40ZLncRNA PVT1 promotes cervical cancer progression by sponging miR-503 to upregulate ARL2 expression2391-541210.1515/biol-2021-0002https://doaj.org/article/4b435add956d45e59deb6153dfc15a412021-01-01T00:00:00Zhttps://doi.org/10.1515/biol-2021-0002https://doaj.org/toc/2391-5412Cervical cancer (CC) is a huge threat to the health of women worldwide. Long non-coding RNA plasmacytoma variant translocation 1 gene (PVT1) was proved to be associated with the development of diverse human cancers, including CC. Nevertheless, the exact mechanism of PVT1 in CC progression remains unclear. Levels of PVT1, microRNA-503 (miR-503), and ADP ribosylation factor-like protein 2 (ARL2) were measured by quantitative reverse transcription-polymerase chain reaction or western blot assay. 3-(4,5)-Dimethylthiazole-2-y1)-2,5-biphenyl tetrazolium bromide (MTT) and flow cytometry were used to examine cell viability and apoptosis, respectively. For migration and invasion detection, transwell assay was performed. The interaction between miR-503 and PVT1 or ARL2 was shown by dual luciferase reporter assay. A nude mouse model was constructed to clarify the role of PVT1 in vivo. PVT1 and ARL2 expressions were increased, whereas miR-503 expression was decreased in CC tissues and cells. PVT1 was a sponge of miR-503, and miR-503 targeted ARL2. PVT1 knockdown suppressed proliferation, migration, and invasion of CC cells, which could be largely reverted by miR-503 inhibitor. In addition, upregulated ARL2 could attenuate si-PVT1-mediated anti-proliferation and anti-metastasis effects on CC cells. Silenced PVT1 also inhibited CC tumor growth in vivo. PVT1 knockdown exerted tumor suppressor role in CC progression via the miR-503/ARL2 axis, at least in part.Liu WeiweiYao DongmeiHuang BoDe Gruyterarticlelncrna pvt1mir-503arl2cervical cancerprogressionBiology (General)QH301-705.5ENOpen Life Sciences, Vol 16, Iss 1, Pp 1-13 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	lncrna pvt1 mir-503 arl2 cervical cancer progression Biology (General) QH301-705.5
spellingShingle	lncrna pvt1 mir-503 arl2 cervical cancer progression Biology (General) QH301-705.5 Liu Weiwei Yao Dongmei Huang Bo LncRNA PVT1 promotes cervical cancer progression by sponging miR-503 to upregulate ARL2 expression
description	Cervical cancer (CC) is a huge threat to the health of women worldwide. Long non-coding RNA plasmacytoma variant translocation 1 gene (PVT1) was proved to be associated with the development of diverse human cancers, including CC. Nevertheless, the exact mechanism of PVT1 in CC progression remains unclear. Levels of PVT1, microRNA-503 (miR-503), and ADP ribosylation factor-like protein 2 (ARL2) were measured by quantitative reverse transcription-polymerase chain reaction or western blot assay. 3-(4,5)-Dimethylthiazole-2-y1)-2,5-biphenyl tetrazolium bromide (MTT) and flow cytometry were used to examine cell viability and apoptosis, respectively. For migration and invasion detection, transwell assay was performed. The interaction between miR-503 and PVT1 or ARL2 was shown by dual luciferase reporter assay. A nude mouse model was constructed to clarify the role of PVT1 in vivo. PVT1 and ARL2 expressions were increased, whereas miR-503 expression was decreased in CC tissues and cells. PVT1 was a sponge of miR-503, and miR-503 targeted ARL2. PVT1 knockdown suppressed proliferation, migration, and invasion of CC cells, which could be largely reverted by miR-503 inhibitor. In addition, upregulated ARL2 could attenuate si-PVT1-mediated anti-proliferation and anti-metastasis effects on CC cells. Silenced PVT1 also inhibited CC tumor growth in vivo. PVT1 knockdown exerted tumor suppressor role in CC progression via the miR-503/ARL2 axis, at least in part.
format	article
author	Liu Weiwei Yao Dongmei Huang Bo
author_facet	Liu Weiwei Yao Dongmei Huang Bo
author_sort	Liu Weiwei
title	LncRNA PVT1 promotes cervical cancer progression by sponging miR-503 to upregulate ARL2 expression
title_short	LncRNA PVT1 promotes cervical cancer progression by sponging miR-503 to upregulate ARL2 expression
title_full	LncRNA PVT1 promotes cervical cancer progression by sponging miR-503 to upregulate ARL2 expression
title_fullStr	LncRNA PVT1 promotes cervical cancer progression by sponging miR-503 to upregulate ARL2 expression
title_full_unstemmed	LncRNA PVT1 promotes cervical cancer progression by sponging miR-503 to upregulate ARL2 expression
title_sort	lncrna pvt1 promotes cervical cancer progression by sponging mir-503 to upregulate arl2 expression
publisher	De Gruyter
publishDate	2021
url	https://doaj.org/article/4b435add956d45e59deb6153dfc15a41
work_keys_str_mv	AT liuweiwei lncrnapvt1promotescervicalcancerprogressionbyspongingmir503toupregulatearl2expression AT yaodongmei lncrnapvt1promotescervicalcancerprogressionbyspongingmir503toupregulatearl2expression AT huangbo lncrnapvt1promotescervicalcancerprogressionbyspongingmir503toupregulatearl2expression
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LncRNA PVT1 promotes cervical cancer progression by sponging miR-503 to upregulate ARL2 expression

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