Osteoprotegerin, pericytes and bone-like vascular calcification are associated with carotid plaque stability.
<h4>Background and purpose</h4>Vascular calcification, recapitulating bone formation, has a profound impact on plaque stability. The aim of the present study was to determine the influence of bone-like vascular calcification (named osteoid metaplasia = OM) and of osteoprotegerin on plaqu...
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2014
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oai:doaj.org-article:4b4a0f94d79845ea8e36fb623f2130692021-11-25T05:59:07ZOsteoprotegerin, pericytes and bone-like vascular calcification are associated with carotid plaque stability.1932-620310.1371/journal.pone.0107642https://doaj.org/article/4b4a0f94d79845ea8e36fb623f2130692014-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0107642https://doaj.org/toc/1932-6203<h4>Background and purpose</h4>Vascular calcification, recapitulating bone formation, has a profound impact on plaque stability. The aim of the present study was to determine the influence of bone-like vascular calcification (named osteoid metaplasia = OM) and of osteoprotegerin on plaque stability.<h4>Methods</h4>Tissue from carotid endarterectomies were analysed for the presence of calcification and signs of vulnerability according to AHA grading system. Osteoprotegerin (OPG), pericytes and endothelial cells were sought using immuno-histochemistry. Symptoms and preoperative imaging findings (CT-scan, MRI and Doppler-scan) were analyzed. Human pericytes were cultured to evaluate their ability to secrete OPG and to influence mineralization in the plaque.<h4>Results</h4>Seventy-three carotid plaques (49 asymptomatic and 24 symptomatic) were harvested. A significantly higher presence of OM (18.4% vs 0%, p<0.01), OPG (10.2% of ROI vs 3.4% of ROI, p<0.05) and pericytes (19% of ROI vs 3.8% of ROI, p<0.05) were noted in asymptomatic compared to symptomatic plaques. Consistently, circulating OPG levels were higher in the plasma of asymptomatic patients (3.2 ng/mL vs 2.5 ng/mL, p = 0.05). In vitro, human vascular pericytes secreted considerable amounts of OPG and underwent osteoblastic differentiation. Pericytes also inhibited the osteoclastic differentiation of CD14+ cells through their secretion of OPG.<h4>Conclusions</h4>OPG (intraplaque an plasmatic) and OM are associated with carotid plaque stability. Pericytes may be involved in the secretion of intraplaque OPG and in the formation of OM.Jean-Michel DavaineThibaut QuillardRégis BrionOlivier LapérineBéatrice GuyomarchThierry MerliniMathias ChatelaisFlorian GuilbaudMeadhbh Áine BrennanCéline CharrierDominique HeymannYann GouëfficMarie-Françoise HeymannPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 9, p e107642 (2014) |
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DOAJ |
| collection |
DOAJ |
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EN |
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Medicine R Science Q |
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Medicine R Science Q Jean-Michel Davaine Thibaut Quillard Régis Brion Olivier Lapérine Béatrice Guyomarch Thierry Merlini Mathias Chatelais Florian Guilbaud Meadhbh Áine Brennan Céline Charrier Dominique Heymann Yann Gouëffic Marie-Françoise Heymann Osteoprotegerin, pericytes and bone-like vascular calcification are associated with carotid plaque stability. |
| description |
<h4>Background and purpose</h4>Vascular calcification, recapitulating bone formation, has a profound impact on plaque stability. The aim of the present study was to determine the influence of bone-like vascular calcification (named osteoid metaplasia = OM) and of osteoprotegerin on plaque stability.<h4>Methods</h4>Tissue from carotid endarterectomies were analysed for the presence of calcification and signs of vulnerability according to AHA grading system. Osteoprotegerin (OPG), pericytes and endothelial cells were sought using immuno-histochemistry. Symptoms and preoperative imaging findings (CT-scan, MRI and Doppler-scan) were analyzed. Human pericytes were cultured to evaluate their ability to secrete OPG and to influence mineralization in the plaque.<h4>Results</h4>Seventy-three carotid plaques (49 asymptomatic and 24 symptomatic) were harvested. A significantly higher presence of OM (18.4% vs 0%, p<0.01), OPG (10.2% of ROI vs 3.4% of ROI, p<0.05) and pericytes (19% of ROI vs 3.8% of ROI, p<0.05) were noted in asymptomatic compared to symptomatic plaques. Consistently, circulating OPG levels were higher in the plasma of asymptomatic patients (3.2 ng/mL vs 2.5 ng/mL, p = 0.05). In vitro, human vascular pericytes secreted considerable amounts of OPG and underwent osteoblastic differentiation. Pericytes also inhibited the osteoclastic differentiation of CD14+ cells through their secretion of OPG.<h4>Conclusions</h4>OPG (intraplaque an plasmatic) and OM are associated with carotid plaque stability. Pericytes may be involved in the secretion of intraplaque OPG and in the formation of OM. |
| format |
article |
| author |
Jean-Michel Davaine Thibaut Quillard Régis Brion Olivier Lapérine Béatrice Guyomarch Thierry Merlini Mathias Chatelais Florian Guilbaud Meadhbh Áine Brennan Céline Charrier Dominique Heymann Yann Gouëffic Marie-Françoise Heymann |
| author_facet |
Jean-Michel Davaine Thibaut Quillard Régis Brion Olivier Lapérine Béatrice Guyomarch Thierry Merlini Mathias Chatelais Florian Guilbaud Meadhbh Áine Brennan Céline Charrier Dominique Heymann Yann Gouëffic Marie-Françoise Heymann |
| author_sort |
Jean-Michel Davaine |
| title |
Osteoprotegerin, pericytes and bone-like vascular calcification are associated with carotid plaque stability. |
| title_short |
Osteoprotegerin, pericytes and bone-like vascular calcification are associated with carotid plaque stability. |
| title_full |
Osteoprotegerin, pericytes and bone-like vascular calcification are associated with carotid plaque stability. |
| title_fullStr |
Osteoprotegerin, pericytes and bone-like vascular calcification are associated with carotid plaque stability. |
| title_full_unstemmed |
Osteoprotegerin, pericytes and bone-like vascular calcification are associated with carotid plaque stability. |
| title_sort |
osteoprotegerin, pericytes and bone-like vascular calcification are associated with carotid plaque stability. |
| publisher |
Public Library of Science (PLoS) |
| publishDate |
2014 |
| url |
https://doaj.org/article/4b4a0f94d79845ea8e36fb623f213069 |
| work_keys_str_mv |
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