Exosomal lncRNA ROR1-AS1 Derived from Tumor Cells Promotes Glioma Progression via Regulating miR-4686

Yang Chai, Hai-Tao Wu, Chuan-Dong Liang, Chun-Yue You, Ming-Xiang Xie, Shun-Wu Xiao Department of Neurosurgery, Affiliated Hospital of Zunyi Medical University, Zunyi, Guizhou 563000, People’s Republic of ChinaCorrespondence: Shun-Wu XiaoDepartment of Neurosurgery, Affiliated Hospital of Z...

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Autores principales:	Chai Y, Wu HT, Liang CD, You CY, Xie MX, Xiao SW
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2020
Materias:	glioma lncrna ror1-as1 exosome mir-4686 tumorigenesis Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/4b4ab0caa2784d50b7935b8c22a94878
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spelling	oai:doaj.org-article:4b4ab0caa2784d50b7935b8c22a948782021-12-02T15:04:57ZExosomal lncRNA ROR1-AS1 Derived from Tumor Cells Promotes Glioma Progression via Regulating miR-46861178-2013https://doaj.org/article/4b4ab0caa2784d50b7935b8c22a948782020-11-01T00:00:00Zhttps://www.dovepress.com/exosomal-lncrna-ror1-as1-derived-from-tumor-cells-promotes-glioma-prog-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Yang Chai, Hai-Tao Wu, Chuan-Dong Liang, Chun-Yue You, Ming-Xiang Xie, Shun-Wu Xiao Department of Neurosurgery, Affiliated Hospital of Zunyi Medical University, Zunyi, Guizhou 563000, People’s Republic of ChinaCorrespondence: Shun-Wu XiaoDepartment of Neurosurgery, Affiliated Hospital of Zunyi Medical University, Zunyi, Guizhou 563000, People’s Republic of ChinaEmail xiaoshunwu34576346@163.comObjective: Glioma is one of the most common central nervous system malignant tumors, accounting for 45%– 60% of adult intracranial tumors. However, the clinical treatment of glioma is limited. It is of great significance to seek new therapeutic methods for glioma via gene therapy.Materials and Methods: Microarray is used to identify the lncRNAs that are differentially expressed in glioma. The expression of long non-coding RNA (lncRNA) ROR1-AS1 and miR-4686 was detected by qRT-PCR. Exosomes were isolated from the supernatant of normal and cancerous cells, and TEM was used for exosomes identification. MTT assay, wound healing assay, transwell assay, and colony formation assay were used to detect the exo-ROR1-AS1 function on proliferation, migration, and invasion in glioma cells. Luciferase assay and RIP assay were used to identify the relationship between lncRNA ROR1-AS1 and miR-4686. The effect of exo-ROR1-AS1 on tumorigenesis of glioma was confirmed by the xenograft nude mice model.Results: ROR1-AS1 was up-regulated in glioma tissues, and the high expression of ROR1-AS1 indicated a poor prognosis in glioma patients. Interestingly, ROR1-AS1 was packaged into exosomes and derived from tumor cells. Functional analysis showed exo-ROR1-AS1 promoted the progression of glioma cell lines SHG44 and U251. Furthermore, ROR1-AS1 acted as a sponge of miR-4686 and inhibited its expression. Functionally, forced expression of miR-4686 removed the promoted effects of lncRNA ROR1-AS1 on glioma development. In vivo tumorigenesis experiments showed that exo-ROR1-AS1 promoted glioma development via miR-4686 axis.Conclusion: Our study suggested tumor cells derived exo-ROR1-AS1 promoted glioma progression by inhibiting miR-4686, which might be a potential therapeutic target for glioma clinical treatment.Keywords: glioma, lncRNA ROR1-AS1, exosome, miR-4686, tumorigenesisChai YWu HTLiang CDYou CYXie MXXiao SWDove Medical Pressarticlegliomalncrna ror1-as1exosomemir-4686tumorigenesisMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 15, Pp 8863-8872 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	glioma lncrna ror1-as1 exosome mir-4686 tumorigenesis Medicine (General) R5-920
spellingShingle	glioma lncrna ror1-as1 exosome mir-4686 tumorigenesis Medicine (General) R5-920 Chai Y Wu HT Liang CD You CY Xie MX Xiao SW Exosomal lncRNA ROR1-AS1 Derived from Tumor Cells Promotes Glioma Progression via Regulating miR-4686
description	Yang Chai, Hai-Tao Wu, Chuan-Dong Liang, Chun-Yue You, Ming-Xiang Xie, Shun-Wu Xiao Department of Neurosurgery, Affiliated Hospital of Zunyi Medical University, Zunyi, Guizhou 563000, People’s Republic of ChinaCorrespondence: Shun-Wu XiaoDepartment of Neurosurgery, Affiliated Hospital of Zunyi Medical University, Zunyi, Guizhou 563000, People’s Republic of ChinaEmail xiaoshunwu34576346@163.comObjective: Glioma is one of the most common central nervous system malignant tumors, accounting for 45%– 60% of adult intracranial tumors. However, the clinical treatment of glioma is limited. It is of great significance to seek new therapeutic methods for glioma via gene therapy.Materials and Methods: Microarray is used to identify the lncRNAs that are differentially expressed in glioma. The expression of long non-coding RNA (lncRNA) ROR1-AS1 and miR-4686 was detected by qRT-PCR. Exosomes were isolated from the supernatant of normal and cancerous cells, and TEM was used for exosomes identification. MTT assay, wound healing assay, transwell assay, and colony formation assay were used to detect the exo-ROR1-AS1 function on proliferation, migration, and invasion in glioma cells. Luciferase assay and RIP assay were used to identify the relationship between lncRNA ROR1-AS1 and miR-4686. The effect of exo-ROR1-AS1 on tumorigenesis of glioma was confirmed by the xenograft nude mice model.Results: ROR1-AS1 was up-regulated in glioma tissues, and the high expression of ROR1-AS1 indicated a poor prognosis in glioma patients. Interestingly, ROR1-AS1 was packaged into exosomes and derived from tumor cells. Functional analysis showed exo-ROR1-AS1 promoted the progression of glioma cell lines SHG44 and U251. Furthermore, ROR1-AS1 acted as a sponge of miR-4686 and inhibited its expression. Functionally, forced expression of miR-4686 removed the promoted effects of lncRNA ROR1-AS1 on glioma development. In vivo tumorigenesis experiments showed that exo-ROR1-AS1 promoted glioma development via miR-4686 axis.Conclusion: Our study suggested tumor cells derived exo-ROR1-AS1 promoted glioma progression by inhibiting miR-4686, which might be a potential therapeutic target for glioma clinical treatment.Keywords: glioma, lncRNA ROR1-AS1, exosome, miR-4686, tumorigenesis
format	article
author	Chai Y Wu HT Liang CD You CY Xie MX Xiao SW
author_facet	Chai Y Wu HT Liang CD You CY Xie MX Xiao SW
author_sort	Chai Y
title	Exosomal lncRNA ROR1-AS1 Derived from Tumor Cells Promotes Glioma Progression via Regulating miR-4686
title_short	Exosomal lncRNA ROR1-AS1 Derived from Tumor Cells Promotes Glioma Progression via Regulating miR-4686
title_full	Exosomal lncRNA ROR1-AS1 Derived from Tumor Cells Promotes Glioma Progression via Regulating miR-4686
title_fullStr	Exosomal lncRNA ROR1-AS1 Derived from Tumor Cells Promotes Glioma Progression via Regulating miR-4686
title_full_unstemmed	Exosomal lncRNA ROR1-AS1 Derived from Tumor Cells Promotes Glioma Progression via Regulating miR-4686
title_sort	exosomal lncrna ror1-as1 derived from tumor cells promotes glioma progression via regulating mir-4686
publisher	Dove Medical Press
publishDate	2020
url	https://doaj.org/article/4b4ab0caa2784d50b7935b8c22a94878
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Exosomal lncRNA ROR1-AS1 Derived from Tumor Cells Promotes Glioma Progression via Regulating miR-4686

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