A Predicted Mannoprotein Participates in <named-content content-type="genus-species">Cryptococcus gattii</named-content> Capsular Structure

ABSTRACT The yeast-like pathogen Cryptococcus gattii is an etiological agent of cryptococcosis. The major cryptococcal virulence factor is the polysaccharide capsule, which is composed of glucuronoxylomannan (GXM), galactoxylomannan (GalXM), and mannoproteins (MPs). The GXM and GalXM polysaccharides...

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Autores principales:	Julia Catarina Vieira Reuwsaat, Heryk Motta, Ane Wichine Acosta Garcia, Carolina Bettker Vasconcelos, Bárbara Machado Marques, Natália Kronbauer Oliveira, Jéssica Rodrigues, Patrícia Aline Gröhns Ferrareze, Susana Frases, William Lopes, Vanessa Abreu Barcellos, Eamim Daidrê Squizani, Jorge André Horta, Augusto Schrank, Marcio Lourenço Rodrigues, Charley Christian Staats, Marilene Henning Vainstein, Lívia Kmetzsch
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2018
Materias:	Cryptococcus gattii capsular polysaccharide mannoprotein Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/4b5e6e2b0c7b4cf8b8b79b323d57903b
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spelling	oai:doaj.org-article:4b5e6e2b0c7b4cf8b8b79b323d57903b2021-11-15T15:22:14ZA Predicted Mannoprotein Participates in <named-content content-type="genus-species">Cryptococcus gattii</named-content> Capsular Structure10.1128/mSphere.00023-182379-5042https://doaj.org/article/4b5e6e2b0c7b4cf8b8b79b323d57903b2018-04-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/mSphere.00023-18https://doaj.org/toc/2379-5042ABSTRACT The yeast-like pathogen Cryptococcus gattii is an etiological agent of cryptococcosis. The major cryptococcal virulence factor is the polysaccharide capsule, which is composed of glucuronoxylomannan (GXM), galactoxylomannan (GalXM), and mannoproteins (MPs). The GXM and GalXM polysaccharides have been extensively characterized; however, there is little information about the role of mannoproteins in capsule assembly and their participation in yeast pathogenicity. The present study characterized the function of a predicted mannoprotein from C. gattii, designated Krp1. Loss-of-function and gain-of-function mutants were generated, and phenotypes associated with the capsular architecture were evaluated. The null mutant cells were more sensitive to a cell wall stressor that disrupts beta-glucan synthesis. Also, these cells displayed increased GXM release to the culture supernatant than the wild-type strain did. The loss of Krp1 influenced cell-associated cryptococcal polysaccharide thickness and phagocytosis by J774.A1 macrophages in the early hours of interaction, but no difference in virulence in a murine model of cryptococcosis was observed. In addition, recombinant Krp1 was antigenic and differentially recognized by serum from an individual with cryptococcosis, but not with serum from an individual with candidiasis. Taken together, these results indicate that C. gattii Krp1 is important for the cell wall structure, thereby influencing capsule assembly, but is not essential for virulence in vivo. IMPORTANCE Cryptococcus gattii has the ability to escape from the host’s immune system through poorly understood mechanisms and can lead to the death of healthy individuals. The role of mannoproteins in C. gattii pathogenicity is not completely understood. The present work characterized a protein, Kpr1, that is essential for the maintenance of C. gattii main virulence factor, the polysaccharide capsule. Our data contribute to the understanding of the role of Kpr1 in capsule structuring, mainly by modulating the distribution of glucans in C. gattii cell wall.Julia Catarina Vieira ReuwsaatHeryk MottaAne Wichine Acosta GarciaCarolina Bettker VasconcelosBárbara Machado MarquesNatália Kronbauer OliveiraJéssica RodriguesPatrícia Aline Gröhns FerrarezeSusana FrasesWilliam LopesVanessa Abreu BarcellosEamim Daidrê SquizaniJorge André HortaAugusto SchrankMarcio Lourenço RodriguesCharley Christian StaatsMarilene Henning VainsteinLívia KmetzschAmerican Society for MicrobiologyarticleCryptococcus gattiicapsular polysaccharidemannoproteinMicrobiologyQR1-502ENmSphere, Vol 3, Iss 2 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Cryptococcus gattii capsular polysaccharide mannoprotein Microbiology QR1-502
spellingShingle	Cryptococcus gattii capsular polysaccharide mannoprotein Microbiology QR1-502 Julia Catarina Vieira Reuwsaat Heryk Motta Ane Wichine Acosta Garcia Carolina Bettker Vasconcelos Bárbara Machado Marques Natália Kronbauer Oliveira Jéssica Rodrigues Patrícia Aline Gröhns Ferrareze Susana Frases William Lopes Vanessa Abreu Barcellos Eamim Daidrê Squizani Jorge André Horta Augusto Schrank Marcio Lourenço Rodrigues Charley Christian Staats Marilene Henning Vainstein Lívia Kmetzsch A Predicted Mannoprotein Participates in <named-content content-type="genus-species">Cryptococcus gattii</named-content> Capsular Structure
description	ABSTRACT The yeast-like pathogen Cryptococcus gattii is an etiological agent of cryptococcosis. The major cryptococcal virulence factor is the polysaccharide capsule, which is composed of glucuronoxylomannan (GXM), galactoxylomannan (GalXM), and mannoproteins (MPs). The GXM and GalXM polysaccharides have been extensively characterized; however, there is little information about the role of mannoproteins in capsule assembly and their participation in yeast pathogenicity. The present study characterized the function of a predicted mannoprotein from C. gattii, designated Krp1. Loss-of-function and gain-of-function mutants were generated, and phenotypes associated with the capsular architecture were evaluated. The null mutant cells were more sensitive to a cell wall stressor that disrupts beta-glucan synthesis. Also, these cells displayed increased GXM release to the culture supernatant than the wild-type strain did. The loss of Krp1 influenced cell-associated cryptococcal polysaccharide thickness and phagocytosis by J774.A1 macrophages in the early hours of interaction, but no difference in virulence in a murine model of cryptococcosis was observed. In addition, recombinant Krp1 was antigenic and differentially recognized by serum from an individual with cryptococcosis, but not with serum from an individual with candidiasis. Taken together, these results indicate that C. gattii Krp1 is important for the cell wall structure, thereby influencing capsule assembly, but is not essential for virulence in vivo. IMPORTANCE Cryptococcus gattii has the ability to escape from the host’s immune system through poorly understood mechanisms and can lead to the death of healthy individuals. The role of mannoproteins in C. gattii pathogenicity is not completely understood. The present work characterized a protein, Kpr1, that is essential for the maintenance of C. gattii main virulence factor, the polysaccharide capsule. Our data contribute to the understanding of the role of Kpr1 in capsule structuring, mainly by modulating the distribution of glucans in C. gattii cell wall.
format	article
author	Julia Catarina Vieira Reuwsaat Heryk Motta Ane Wichine Acosta Garcia Carolina Bettker Vasconcelos Bárbara Machado Marques Natália Kronbauer Oliveira Jéssica Rodrigues Patrícia Aline Gröhns Ferrareze Susana Frases William Lopes Vanessa Abreu Barcellos Eamim Daidrê Squizani Jorge André Horta Augusto Schrank Marcio Lourenço Rodrigues Charley Christian Staats Marilene Henning Vainstein Lívia Kmetzsch
author_facet	Julia Catarina Vieira Reuwsaat Heryk Motta Ane Wichine Acosta Garcia Carolina Bettker Vasconcelos Bárbara Machado Marques Natália Kronbauer Oliveira Jéssica Rodrigues Patrícia Aline Gröhns Ferrareze Susana Frases William Lopes Vanessa Abreu Barcellos Eamim Daidrê Squizani Jorge André Horta Augusto Schrank Marcio Lourenço Rodrigues Charley Christian Staats Marilene Henning Vainstein Lívia Kmetzsch
author_sort	Julia Catarina Vieira Reuwsaat
title	A Predicted Mannoprotein Participates in <named-content content-type="genus-species">Cryptococcus gattii</named-content> Capsular Structure
title_short	A Predicted Mannoprotein Participates in <named-content content-type="genus-species">Cryptococcus gattii</named-content> Capsular Structure
title_full	A Predicted Mannoprotein Participates in <named-content content-type="genus-species">Cryptococcus gattii</named-content> Capsular Structure
title_fullStr	A Predicted Mannoprotein Participates in <named-content content-type="genus-species">Cryptococcus gattii</named-content> Capsular Structure
title_full_unstemmed	A Predicted Mannoprotein Participates in <named-content content-type="genus-species">Cryptococcus gattii</named-content> Capsular Structure
title_sort	predicted mannoprotein participates in <named-content content-type="genus-species">cryptococcus gattii</named-content> capsular structure
publisher	American Society for Microbiology
publishDate	2018
url	https://doaj.org/article/4b5e6e2b0c7b4cf8b8b79b323d57903b
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A Predicted Mannoprotein Participates in <named-content content-type="genus-species">Cryptococcus gattii</named-content> Capsular Structure

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