Chronic marijuana usage by human pancreas donors is associated with impaired islet function

We investigated the effect of chronic marijuana use, defined as 4 times weekly for more than 3 years, on human pancreatic islets. Pancreata from deceased donors who chronically used marijuana were compared to those from age, sex and ethnicity matched non-users. The islets from marijuana-users displa...

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Autores principales:	Meirigeng Qi, John S. Kaddis, Kuan-Tsen Chen, Jeffrey Rawson, Keiko Omori, Zhen Bouman Chen, Sangeeta Dhawan, Jeffrey S. Isenberg, Fouad Kandeel, Bart O. Roep, Ismail H. Al-Abdullah
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/4b7a770ce1a7403aa1ed7fa083396837
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spelling	oai:doaj.org-article:4b7a770ce1a7403aa1ed7fa0833968372021-11-04T06:49:41ZChronic marijuana usage by human pancreas donors is associated with impaired islet function1932-6203https://doaj.org/article/4b7a770ce1a7403aa1ed7fa0833968372021-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC8550598/?tool=EBIhttps://doaj.org/toc/1932-6203We investigated the effect of chronic marijuana use, defined as 4 times weekly for more than 3 years, on human pancreatic islets. Pancreata from deceased donors who chronically used marijuana were compared to those from age, sex and ethnicity matched non-users. The islets from marijuana-users displayed reduced insulin secretion as compared to islets from non-users upon stimulation with high glucose (AUC, 3.41 ± 0.62 versus 5.14 ±0.47, p<0.05) and high glucose plus KCl (AUC, 4.48 ± 0.41 versus 7.69 ± 0.58, p<0.001). When human islets from chronic marijuana-users were transplanted into diabetic mice, the mean reversal rate of diabetes was 35% versus 77% in animals receiving islets from non-users (p<0.01). Immunofluorescent staining for cannabinoid receptor type 1 (CB1R) was shown to be colocalized with insulin and enhanced significantly in beta cells from marijuana-users vs. non-users (CB1R intensity/islet area, 14.95 ± 2.71 vs. 3.23 ± 0.87, p<0.001). In contrast, CB1R expression was not co-localized with glucagon or somatostatin. Furthermore, isolated islets from chronic marijuana-users appeared hypertrophic. In conclusion, excessive marijuana use affects islet endocrine phenotype and function in vitro and in vivo. Given the increasing use of marijuana, our results underline the importance of including lifestyle when evaluating human islets for transplantation or research.Meirigeng QiJohn S. KaddisKuan-Tsen ChenJeffrey RawsonKeiko OmoriZhen Bouman ChenSangeeta DhawanJeffrey S. IsenbergFouad KandeelBart O. RoepIsmail H. Al-AbdullahPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 16, Iss 10 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Meirigeng Qi John S. Kaddis Kuan-Tsen Chen Jeffrey Rawson Keiko Omori Zhen Bouman Chen Sangeeta Dhawan Jeffrey S. Isenberg Fouad Kandeel Bart O. Roep Ismail H. Al-Abdullah Chronic marijuana usage by human pancreas donors is associated with impaired islet function
description	We investigated the effect of chronic marijuana use, defined as 4 times weekly for more than 3 years, on human pancreatic islets. Pancreata from deceased donors who chronically used marijuana were compared to those from age, sex and ethnicity matched non-users. The islets from marijuana-users displayed reduced insulin secretion as compared to islets from non-users upon stimulation with high glucose (AUC, 3.41 ± 0.62 versus 5.14 ±0.47, p<0.05) and high glucose plus KCl (AUC, 4.48 ± 0.41 versus 7.69 ± 0.58, p<0.001). When human islets from chronic marijuana-users were transplanted into diabetic mice, the mean reversal rate of diabetes was 35% versus 77% in animals receiving islets from non-users (p<0.01). Immunofluorescent staining for cannabinoid receptor type 1 (CB1R) was shown to be colocalized with insulin and enhanced significantly in beta cells from marijuana-users vs. non-users (CB1R intensity/islet area, 14.95 ± 2.71 vs. 3.23 ± 0.87, p<0.001). In contrast, CB1R expression was not co-localized with glucagon or somatostatin. Furthermore, isolated islets from chronic marijuana-users appeared hypertrophic. In conclusion, excessive marijuana use affects islet endocrine phenotype and function in vitro and in vivo. Given the increasing use of marijuana, our results underline the importance of including lifestyle when evaluating human islets for transplantation or research.
format	article
author	Meirigeng Qi John S. Kaddis Kuan-Tsen Chen Jeffrey Rawson Keiko Omori Zhen Bouman Chen Sangeeta Dhawan Jeffrey S. Isenberg Fouad Kandeel Bart O. Roep Ismail H. Al-Abdullah
author_facet	Meirigeng Qi John S. Kaddis Kuan-Tsen Chen Jeffrey Rawson Keiko Omori Zhen Bouman Chen Sangeeta Dhawan Jeffrey S. Isenberg Fouad Kandeel Bart O. Roep Ismail H. Al-Abdullah
author_sort	Meirigeng Qi
title	Chronic marijuana usage by human pancreas donors is associated with impaired islet function
title_short	Chronic marijuana usage by human pancreas donors is associated with impaired islet function
title_full	Chronic marijuana usage by human pancreas donors is associated with impaired islet function
title_fullStr	Chronic marijuana usage by human pancreas donors is associated with impaired islet function
title_full_unstemmed	Chronic marijuana usage by human pancreas donors is associated with impaired islet function
title_sort	chronic marijuana usage by human pancreas donors is associated with impaired islet function
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/4b7a770ce1a7403aa1ed7fa083396837
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Chronic marijuana usage by human pancreas donors is associated with impaired islet function

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