Comparative transcriptome analysis of inner blood-retinal barrier and blood–brain barrier in rats

Abstract Although retinal microvessels (RMVs) and brain microvessels (BMVs) are closely related in their developmental and share similar blood-neural barriers, studies have reported markedly different responses to stressors such as diabetes. Therefore, we hypothesized that RMVs and BMVs will display...

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Autores principales:	Y. Li, A. Faiz, H. Moshage, R. Schubert, L. Schilling, J. A. Kamps
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/4b7c85722c3e4e8c80bde377bf1b7f32
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spelling	oai:doaj.org-article:4b7c85722c3e4e8c80bde377bf1b7f322021-12-02T14:59:37ZComparative transcriptome analysis of inner blood-retinal barrier and blood–brain barrier in rats10.1038/s41598-021-91584-72045-2322https://doaj.org/article/4b7c85722c3e4e8c80bde377bf1b7f322021-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-91584-7https://doaj.org/toc/2045-2322Abstract Although retinal microvessels (RMVs) and brain microvessels (BMVs) are closely related in their developmental and share similar blood-neural barriers, studies have reported markedly different responses to stressors such as diabetes. Therefore, we hypothesized that RMVs and BMVs will display substantial differences in gene expression levels even though they are of the same embryological origin. In this study, both RMVs and BMVs were mechanically isolated from rats. Full retinal and brain tissue samples (RT, BT) were collected for comparisons. Total RNA extracted from these four groups were processed on Affymetrix rat 2.0 microarray Chips. The transcriptional profiles of these tissues were then analyzed. In the present paper we looked at differentially expressed genes (DEGs) in RMVs (against RT) and BMVs (against BT) using a rather conservative threshold value of ≥ ± twofold change and a false discovery rate corrected for multiple comparisons (p < 0.05). In RMVs a total of 1559 DEGs were found, of which 1004 genes were higher expressed in RMVs than in RT. Moreover, 4244 DEGs between BMVs and BT were identified, of which 1956 genes were ≥ twofold enriched in BMVs. Using these DEGs, we comprehensively analyzed the actual expression levels and highlighted their involvement in critical functional structures in RMVs and BMVs, such as junctional complex, transporters and signaling pathways. Our work provides for the first time the transcriptional profiles of rat RMVs and BMVs. These results may help to understand why retina and brain microvasculature show different susceptibilities to stressors, and they might even provide new insight for pharmacological interventions.Y. LiA. FaizH. MoshageR. SchubertL. SchillingJ. A. KampsNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-13 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Y. Li A. Faiz H. Moshage R. Schubert L. Schilling J. A. Kamps Comparative transcriptome analysis of inner blood-retinal barrier and blood–brain barrier in rats
description	Abstract Although retinal microvessels (RMVs) and brain microvessels (BMVs) are closely related in their developmental and share similar blood-neural barriers, studies have reported markedly different responses to stressors such as diabetes. Therefore, we hypothesized that RMVs and BMVs will display substantial differences in gene expression levels even though they are of the same embryological origin. In this study, both RMVs and BMVs were mechanically isolated from rats. Full retinal and brain tissue samples (RT, BT) were collected for comparisons. Total RNA extracted from these four groups were processed on Affymetrix rat 2.0 microarray Chips. The transcriptional profiles of these tissues were then analyzed. In the present paper we looked at differentially expressed genes (DEGs) in RMVs (against RT) and BMVs (against BT) using a rather conservative threshold value of ≥ ± twofold change and a false discovery rate corrected for multiple comparisons (p < 0.05). In RMVs a total of 1559 DEGs were found, of which 1004 genes were higher expressed in RMVs than in RT. Moreover, 4244 DEGs between BMVs and BT were identified, of which 1956 genes were ≥ twofold enriched in BMVs. Using these DEGs, we comprehensively analyzed the actual expression levels and highlighted their involvement in critical functional structures in RMVs and BMVs, such as junctional complex, transporters and signaling pathways. Our work provides for the first time the transcriptional profiles of rat RMVs and BMVs. These results may help to understand why retina and brain microvasculature show different susceptibilities to stressors, and they might even provide new insight for pharmacological interventions.
format	article
author	Y. Li A. Faiz H. Moshage R. Schubert L. Schilling J. A. Kamps
author_facet	Y. Li A. Faiz H. Moshage R. Schubert L. Schilling J. A. Kamps
author_sort	Y. Li
title	Comparative transcriptome analysis of inner blood-retinal barrier and blood–brain barrier in rats
title_short	Comparative transcriptome analysis of inner blood-retinal barrier and blood–brain barrier in rats
title_full	Comparative transcriptome analysis of inner blood-retinal barrier and blood–brain barrier in rats
title_fullStr	Comparative transcriptome analysis of inner blood-retinal barrier and blood–brain barrier in rats
title_full_unstemmed	Comparative transcriptome analysis of inner blood-retinal barrier and blood–brain barrier in rats
title_sort	comparative transcriptome analysis of inner blood-retinal barrier and blood–brain barrier in rats
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/4b7c85722c3e4e8c80bde377bf1b7f32
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Comparative transcriptome analysis of inner blood-retinal barrier and blood–brain barrier in rats

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