Nasal Tissue Extraction Is Essential for Characterization of the Murine Upper Respiratory Tract Microbiota

ABSTRACT Respiratory infections are a leading cause of morbidity and mortality worldwide. Bacterial pathogens often colonize the upper respiratory tract (nose or mouth) prior to causing lower respiratory infections or invasive disease. Interactions within the upper respiratory tract between colonizi...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	L. Patrick Schenck, Joshua J. C. McGrath, Daphnée Lamarche, Martin R. Stämpfli, Dawn M. E. Bowdish, Michael G. Surette
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2020
Materias:	Streptococcus pneumoniae colonization microbiota upper respiratory tract Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/4b8080cc2100449f9a7fc427badf0db7
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:4b8080cc2100449f9a7fc427badf0db7
record_format	dspace
spelling	oai:doaj.org-article:4b8080cc2100449f9a7fc427badf0db72021-11-15T15:31:12ZNasal Tissue Extraction Is Essential for Characterization of the Murine Upper Respiratory Tract Microbiota10.1128/mSphere.00562-202379-5042https://doaj.org/article/4b8080cc2100449f9a7fc427badf0db72020-12-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/mSphere.00562-20https://doaj.org/toc/2379-5042ABSTRACT Respiratory infections are a leading cause of morbidity and mortality worldwide. Bacterial pathogens often colonize the upper respiratory tract (nose or mouth) prior to causing lower respiratory infections or invasive disease. Interactions within the upper respiratory tract between colonizing bacteria and the resident microbiota could contribute to colonization success and subsequent transmission. Human carriage studies have identified associations between pathogens such as Streptococcus pneumoniae and members of the resident microbiota, although few mechanisms of competition and cooperation have been identified and would be aided by the use of animal models. Little is known about the composition of the murine nasal microbiota; thus, we set out to improve assessment, including tissue sampling, composition, and comparison between mouse sources. Nasal washes were efficient in sampling the nasopharyngeal space but barely disrupted the nasal turbinates. Nasal tissue extraction increased the yield of cultivable bacterial compared to nasal washes, revealing distinct community compositions. Experimental pneumococcal colonization led to dominance by the colonizing pathogen in the nasopharynx and nasal turbinates, but the composition of the microbiota, and interactions with resident microbes, differed depending on the sampling method. Importantly, vendor source has a large impact on microbial composition. Bacterial interactions, including cooperation and colonization resistance, depend on the biogeography of the nose and should be considered during research design of experimental colonization with pathogens. IMPORTANCE The nasal microbiota is composed of species that play a role in the colonization success of pathogens, including Streptococcus pneumoniae and Staphylococcus aureus. Murine models provide the ability to explore disease pathogenesis, but little is known about the natural murine nasal microbiota. This study established techniques to allow the exploration of the bacterial members of the nasal microbiota. The mouse nasal microbiota included traditional respiratory bacteria, including Streptococcus, Staphylococcus, and Moraxella species. Analyses were affected by different sampling methods as well as the commercial source of the mice, which should be included in future research design of infectious disease research.L. Patrick SchenckJoshua J. C. McGrathDaphnée LamarcheMartin R. StämpfliDawn M. E. BowdishMichael G. SuretteAmerican Society for MicrobiologyarticleStreptococcus pneumoniaecolonizationmicrobiotaupper respiratory tractMicrobiologyQR1-502ENmSphere, Vol 5, Iss 6 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Streptococcus pneumoniae colonization microbiota upper respiratory tract Microbiology QR1-502
spellingShingle	Streptococcus pneumoniae colonization microbiota upper respiratory tract Microbiology QR1-502 L. Patrick Schenck Joshua J. C. McGrath Daphnée Lamarche Martin R. Stämpfli Dawn M. E. Bowdish Michael G. Surette Nasal Tissue Extraction Is Essential for Characterization of the Murine Upper Respiratory Tract Microbiota
description	ABSTRACT Respiratory infections are a leading cause of morbidity and mortality worldwide. Bacterial pathogens often colonize the upper respiratory tract (nose or mouth) prior to causing lower respiratory infections or invasive disease. Interactions within the upper respiratory tract between colonizing bacteria and the resident microbiota could contribute to colonization success and subsequent transmission. Human carriage studies have identified associations between pathogens such as Streptococcus pneumoniae and members of the resident microbiota, although few mechanisms of competition and cooperation have been identified and would be aided by the use of animal models. Little is known about the composition of the murine nasal microbiota; thus, we set out to improve assessment, including tissue sampling, composition, and comparison between mouse sources. Nasal washes were efficient in sampling the nasopharyngeal space but barely disrupted the nasal turbinates. Nasal tissue extraction increased the yield of cultivable bacterial compared to nasal washes, revealing distinct community compositions. Experimental pneumococcal colonization led to dominance by the colonizing pathogen in the nasopharynx and nasal turbinates, but the composition of the microbiota, and interactions with resident microbes, differed depending on the sampling method. Importantly, vendor source has a large impact on microbial composition. Bacterial interactions, including cooperation and colonization resistance, depend on the biogeography of the nose and should be considered during research design of experimental colonization with pathogens. IMPORTANCE The nasal microbiota is composed of species that play a role in the colonization success of pathogens, including Streptococcus pneumoniae and Staphylococcus aureus. Murine models provide the ability to explore disease pathogenesis, but little is known about the natural murine nasal microbiota. This study established techniques to allow the exploration of the bacterial members of the nasal microbiota. The mouse nasal microbiota included traditional respiratory bacteria, including Streptococcus, Staphylococcus, and Moraxella species. Analyses were affected by different sampling methods as well as the commercial source of the mice, which should be included in future research design of infectious disease research.
format	article
author	L. Patrick Schenck Joshua J. C. McGrath Daphnée Lamarche Martin R. Stämpfli Dawn M. E. Bowdish Michael G. Surette
author_facet	L. Patrick Schenck Joshua J. C. McGrath Daphnée Lamarche Martin R. Stämpfli Dawn M. E. Bowdish Michael G. Surette
author_sort	L. Patrick Schenck
title	Nasal Tissue Extraction Is Essential for Characterization of the Murine Upper Respiratory Tract Microbiota
title_short	Nasal Tissue Extraction Is Essential for Characterization of the Murine Upper Respiratory Tract Microbiota
title_full	Nasal Tissue Extraction Is Essential for Characterization of the Murine Upper Respiratory Tract Microbiota
title_fullStr	Nasal Tissue Extraction Is Essential for Characterization of the Murine Upper Respiratory Tract Microbiota
title_full_unstemmed	Nasal Tissue Extraction Is Essential for Characterization of the Murine Upper Respiratory Tract Microbiota
title_sort	nasal tissue extraction is essential for characterization of the murine upper respiratory tract microbiota
publisher	American Society for Microbiology
publishDate	2020
url	https://doaj.org/article/4b8080cc2100449f9a7fc427badf0db7
work_keys_str_mv	AT lpatrickschenck nasaltissueextractionisessentialforcharacterizationofthemurineupperrespiratorytractmicrobiota AT joshuajcmcgrath nasaltissueextractionisessentialforcharacterizationofthemurineupperrespiratorytractmicrobiota AT daphneelamarche nasaltissueextractionisessentialforcharacterizationofthemurineupperrespiratorytractmicrobiota AT martinrstampfli nasaltissueextractionisessentialforcharacterizationofthemurineupperrespiratorytractmicrobiota AT dawnmebowdish nasaltissueextractionisessentialforcharacterizationofthemurineupperrespiratorytractmicrobiota AT michaelgsurette nasaltissueextractionisessentialforcharacterizationofthemurineupperrespiratorytractmicrobiota
_version_	1718427823721414656

Nasal Tissue Extraction Is Essential for Characterization of the Murine Upper Respiratory Tract Microbiota

Ejemplares similares