Pulmonary Arterial Hypertension Affects the Rat Gut Microbiome

Abstract We have analysed whether pulmonary arterial hypertension (PAH) alters the rat faecal microbiota. Wistar rats were injected with the VEGF receptor antagonist SU5416 (20 mg/kg s.c.) and followed for 2 weeks kept in hypoxia (10% O2, PAH) or injected with vehicle and kept in normoxia (controls)...

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Autores principales:	María Callejo, Gema Mondejar-Parreño, Bianca Barreira, José L. Izquierdo-Garcia, Daniel Morales-Cano, Sergio Esquivel-Ruiz, Laura Moreno, Ángel Cogolludo, Juan Duarte, Francisco Perez-Vizcaino
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/4b82a1856ded4f90ab2d78077a5e9f99
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spelling	oai:doaj.org-article:4b82a1856ded4f90ab2d78077a5e9f992021-12-02T15:08:37ZPulmonary Arterial Hypertension Affects the Rat Gut Microbiome10.1038/s41598-018-27682-w2045-2322https://doaj.org/article/4b82a1856ded4f90ab2d78077a5e9f992018-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-27682-whttps://doaj.org/toc/2045-2322Abstract We have analysed whether pulmonary arterial hypertension (PAH) alters the rat faecal microbiota. Wistar rats were injected with the VEGF receptor antagonist SU5416 (20 mg/kg s.c.) and followed for 2 weeks kept in hypoxia (10% O2, PAH) or injected with vehicle and kept in normoxia (controls). Faecal samples were obtained and microbiome composition was determined by 16S rRNA gene sequencing and bioinformatic analysis. No effect of PAH on the global microbiome was found (α- or β-diversity). However, PAH-exposed rats showed gut dysbiosis as indicated by a taxonomy-based analysis. Specifically, PAH rats had a three-fold increase in Firmicutes-to-Bacteroidetes ratio. Within the Firmicutes phylum, there were no large changes in the relative abundance of the bacterial families in PAH. Among Bacteroidetes, all families were less abundant in PAH. A clear separation was observed between the control and PAH clusters based on short chain fatty acid producing bacterial genera. Moreover, acetate was reduced in the serum of PAH rats. In conclusion, faecal microbiota composition is altered as a result of PAH. This misbalanced bacterial ecosystem might in turn play a pathophysiological role in PAH by altering the immunologic, hormonal and metabolic homeostasis.María CallejoGema Mondejar-ParreñoBianca BarreiraJosé L. Izquierdo-GarciaDaniel Morales-CanoSergio Esquivel-RuizLaura MorenoÁngel CogolludoJuan DuarteFrancisco Perez-VizcainoNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-10 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q María Callejo Gema Mondejar-Parreño Bianca Barreira José L. Izquierdo-Garcia Daniel Morales-Cano Sergio Esquivel-Ruiz Laura Moreno Ángel Cogolludo Juan Duarte Francisco Perez-Vizcaino Pulmonary Arterial Hypertension Affects the Rat Gut Microbiome
description	Abstract We have analysed whether pulmonary arterial hypertension (PAH) alters the rat faecal microbiota. Wistar rats were injected with the VEGF receptor antagonist SU5416 (20 mg/kg s.c.) and followed for 2 weeks kept in hypoxia (10% O2, PAH) or injected with vehicle and kept in normoxia (controls). Faecal samples were obtained and microbiome composition was determined by 16S rRNA gene sequencing and bioinformatic analysis. No effect of PAH on the global microbiome was found (α- or β-diversity). However, PAH-exposed rats showed gut dysbiosis as indicated by a taxonomy-based analysis. Specifically, PAH rats had a three-fold increase in Firmicutes-to-Bacteroidetes ratio. Within the Firmicutes phylum, there were no large changes in the relative abundance of the bacterial families in PAH. Among Bacteroidetes, all families were less abundant in PAH. A clear separation was observed between the control and PAH clusters based on short chain fatty acid producing bacterial genera. Moreover, acetate was reduced in the serum of PAH rats. In conclusion, faecal microbiota composition is altered as a result of PAH. This misbalanced bacterial ecosystem might in turn play a pathophysiological role in PAH by altering the immunologic, hormonal and metabolic homeostasis.
format	article
author	María Callejo Gema Mondejar-Parreño Bianca Barreira José L. Izquierdo-Garcia Daniel Morales-Cano Sergio Esquivel-Ruiz Laura Moreno Ángel Cogolludo Juan Duarte Francisco Perez-Vizcaino
author_facet	María Callejo Gema Mondejar-Parreño Bianca Barreira José L. Izquierdo-Garcia Daniel Morales-Cano Sergio Esquivel-Ruiz Laura Moreno Ángel Cogolludo Juan Duarte Francisco Perez-Vizcaino
author_sort	María Callejo
title	Pulmonary Arterial Hypertension Affects the Rat Gut Microbiome
title_short	Pulmonary Arterial Hypertension Affects the Rat Gut Microbiome
title_full	Pulmonary Arterial Hypertension Affects the Rat Gut Microbiome
title_fullStr	Pulmonary Arterial Hypertension Affects the Rat Gut Microbiome
title_full_unstemmed	Pulmonary Arterial Hypertension Affects the Rat Gut Microbiome
title_sort	pulmonary arterial hypertension affects the rat gut microbiome
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/4b82a1856ded4f90ab2d78077a5e9f99
work_keys_str_mv	AT mariacallejo pulmonaryarterialhypertensionaffectstheratgutmicrobiome AT gemamondejarparreno pulmonaryarterialhypertensionaffectstheratgutmicrobiome AT biancabarreira pulmonaryarterialhypertensionaffectstheratgutmicrobiome AT joselizquierdogarcia pulmonaryarterialhypertensionaffectstheratgutmicrobiome AT danielmoralescano pulmonaryarterialhypertensionaffectstheratgutmicrobiome AT sergioesquivelruiz pulmonaryarterialhypertensionaffectstheratgutmicrobiome AT lauramoreno pulmonaryarterialhypertensionaffectstheratgutmicrobiome AT angelcogolludo pulmonaryarterialhypertensionaffectstheratgutmicrobiome AT juanduarte pulmonaryarterialhypertensionaffectstheratgutmicrobiome AT franciscoperezvizcaino pulmonaryarterialhypertensionaffectstheratgutmicrobiome
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Pulmonary Arterial Hypertension Affects the Rat Gut Microbiome

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