Silencing CDK6-AS1 inhibits LPS-induced inflammatory damage in HK-2 cells

In this study, we aim to discover the importance of long non-coding RNA cyclin-dependent kinase 6 (CDK6)-AS1 in lipopolysaccharide (LPS)-induced HK-2 cells. We treated the HK-2 cells with LPS and knocked down CDK6-AS1 in HK-2 cells and then analyzed the effects of CDK6-AS1 on the viability of cell,...

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Autores principales:	Wu Ling, Zhang Rui, Lin Sheng, Lin Min, Wang Jing
Formato:	article
Lenguaje:	EN
Publicado:	De Gruyter 2021
Materias:	long non-coding rna lps cdk6-as1 apoptosis Medicine R
Acceso en línea:	https://doaj.org/article/4b84cee53ce249bc8f9ffd1875f9c8ab
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spelling	oai:doaj.org-article:4b84cee53ce249bc8f9ffd1875f9c8ab2021-12-05T14:10:55ZSilencing CDK6-AS1 inhibits LPS-induced inflammatory damage in HK-2 cells2391-546310.1515/med-2021-0314https://doaj.org/article/4b84cee53ce249bc8f9ffd1875f9c8ab2021-08-01T00:00:00Zhttps://doi.org/10.1515/med-2021-0314https://doaj.org/toc/2391-5463In this study, we aim to discover the importance of long non-coding RNA cyclin-dependent kinase 6 (CDK6)-AS1 in lipopolysaccharide (LPS)-induced HK-2 cells. We treated the HK-2 cells with LPS and knocked down CDK6-AS1 in HK-2 cells and then analyzed the effects of CDK6-AS1 on the viability of cell, cell apoptosis, the expression of cytokines via MTT, flow cytometry, enzyme-linked immunosorbent assay (ELISA), and qPCR. The results showed that silencing CDK6-AS1 alleviated LPS-induced inhibition of HK-2 cell proliferation, release of IL-1β, IL-8, IL-6, and TNF-α, cell apoptosis, and decrease in mitochondrial membrane potential. In addition, decreasing the level of CDK6-AS1 inhibited the reduction of Bcl-2 levels, the expression of Bax, cleaved caspade-9, and cleaved caspase-3, induced by LPS. In conclusion, lowering CDK6-AS1 level alleviates LPS-induced inflammatory damage in HK-2 cells.Wu LingZhang RuiLin ShengLin MinWang JingDe Gruyterarticlelong non-coding rnalpscdk6-as1apoptosisMedicineRENOpen Medicine, Vol 16, Iss 1, Pp 1256-1264 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	long non-coding rna lps cdk6-as1 apoptosis Medicine R
spellingShingle	long non-coding rna lps cdk6-as1 apoptosis Medicine R Wu Ling Zhang Rui Lin Sheng Lin Min Wang Jing Silencing CDK6-AS1 inhibits LPS-induced inflammatory damage in HK-2 cells
description	In this study, we aim to discover the importance of long non-coding RNA cyclin-dependent kinase 6 (CDK6)-AS1 in lipopolysaccharide (LPS)-induced HK-2 cells. We treated the HK-2 cells with LPS and knocked down CDK6-AS1 in HK-2 cells and then analyzed the effects of CDK6-AS1 on the viability of cell, cell apoptosis, the expression of cytokines via MTT, flow cytometry, enzyme-linked immunosorbent assay (ELISA), and qPCR. The results showed that silencing CDK6-AS1 alleviated LPS-induced inhibition of HK-2 cell proliferation, release of IL-1β, IL-8, IL-6, and TNF-α, cell apoptosis, and decrease in mitochondrial membrane potential. In addition, decreasing the level of CDK6-AS1 inhibited the reduction of Bcl-2 levels, the expression of Bax, cleaved caspade-9, and cleaved caspase-3, induced by LPS. In conclusion, lowering CDK6-AS1 level alleviates LPS-induced inflammatory damage in HK-2 cells.
format	article
author	Wu Ling Zhang Rui Lin Sheng Lin Min Wang Jing
author_facet	Wu Ling Zhang Rui Lin Sheng Lin Min Wang Jing
author_sort	Wu Ling
title	Silencing CDK6-AS1 inhibits LPS-induced inflammatory damage in HK-2 cells
title_short	Silencing CDK6-AS1 inhibits LPS-induced inflammatory damage in HK-2 cells
title_full	Silencing CDK6-AS1 inhibits LPS-induced inflammatory damage in HK-2 cells
title_fullStr	Silencing CDK6-AS1 inhibits LPS-induced inflammatory damage in HK-2 cells
title_full_unstemmed	Silencing CDK6-AS1 inhibits LPS-induced inflammatory damage in HK-2 cells
title_sort	silencing cdk6-as1 inhibits lps-induced inflammatory damage in hk-2 cells
publisher	De Gruyter
publishDate	2021
url	https://doaj.org/article/4b84cee53ce249bc8f9ffd1875f9c8ab
work_keys_str_mv	AT wuling silencingcdk6as1inhibitslpsinducedinflammatorydamageinhk2cells AT zhangrui silencingcdk6as1inhibitslpsinducedinflammatorydamageinhk2cells AT linsheng silencingcdk6as1inhibitslpsinducedinflammatorydamageinhk2cells AT linmin silencingcdk6as1inhibitslpsinducedinflammatorydamageinhk2cells AT wangjing silencingcdk6as1inhibitslpsinducedinflammatorydamageinhk2cells
_version_	1718371601327587328

Silencing CDK6-AS1 inhibits LPS-induced inflammatory damage in HK-2 cells

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