Culex quinquefasciatus carrying Wolbachia is less susceptible to entomopathogenic bacteria

Abstract In an attempt to evaluate the susceptibility of the mosquito Culex quinquefasciatus to bacterial agents, a population naturally infected with a Wolbachia pipientis wPipSJ native strain was tested against the action of three bacterial mosquitocides, Bacillus thuringiensis subsp. israelensis,...

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Autores principales:	Leonardo M. Díaz-Nieto, M. Florencia Gil, J. Nicolás Lazarte, M. Alejandra Perotti, Corina M. Berón
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/4b879ef4070b4a4d952de83da06125a4
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spelling	oai:doaj.org-article:4b879ef4070b4a4d952de83da06125a42021-12-02T15:23:04ZCulex quinquefasciatus carrying Wolbachia is less susceptible to entomopathogenic bacteria10.1038/s41598-020-80034-52045-2322https://doaj.org/article/4b879ef4070b4a4d952de83da06125a42021-01-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-80034-5https://doaj.org/toc/2045-2322Abstract In an attempt to evaluate the susceptibility of the mosquito Culex quinquefasciatus to bacterial agents, a population naturally infected with a Wolbachia pipientis wPipSJ native strain was tested against the action of three bacterial mosquitocides, Bacillus thuringiensis subsp. israelensis, Bacillus wiedmannii biovar thuringiensis and Lysinibacillus sphaericus. Tests were carried out on mosquito larvae with and without Wolbachia (controls). Cx. quinquefasciatus naturally infected with the native wPipSJ strain proved to be more resistant to the pathogenic action of the three mosquitocidal bacterial strains. Additionally, wPipSJ was fully characterised using metagenome-assembled genomics, PCR–RFLP (PCR-Restriction Fragment Length Polymorphism) and MLST (MultiLocus Sequence Typing) analyses. This Wolbachia strain wPipSJ belongs to haplotype I, group wPip-III and supergroup B, clustering with other mosquito wPip strains, such as wPip PEL, wPip JHB, wPip Mol, and wAlbB; showing the southernmost distribution in America. The cytoplasmic incompatibility phenotype of this strain was revealed via crosses between wildtype (Wolbachia +) and antibiotic treated mosquito populations. The results of the tests with the bacterial agents suggest that Cx. quinquefasciatus naturally infected with wPipSJ is less susceptible to the pathogenic action of mosquitocidal bacterial strains when compared with the antibiotic-treated mosquito isoline, and is more susceptible to B. thuringiensis subsp. israelensis than to the other two mosquitocidal agents.Leonardo M. Díaz-NietoM. Florencia GilJ. Nicolás LazarteM. Alejandra PerottiCorina M. BerónNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-9 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Leonardo M. Díaz-Nieto M. Florencia Gil J. Nicolás Lazarte M. Alejandra Perotti Corina M. Berón Culex quinquefasciatus carrying Wolbachia is less susceptible to entomopathogenic bacteria
description	Abstract In an attempt to evaluate the susceptibility of the mosquito Culex quinquefasciatus to bacterial agents, a population naturally infected with a Wolbachia pipientis wPipSJ native strain was tested against the action of three bacterial mosquitocides, Bacillus thuringiensis subsp. israelensis, Bacillus wiedmannii biovar thuringiensis and Lysinibacillus sphaericus. Tests were carried out on mosquito larvae with and without Wolbachia (controls). Cx. quinquefasciatus naturally infected with the native wPipSJ strain proved to be more resistant to the pathogenic action of the three mosquitocidal bacterial strains. Additionally, wPipSJ was fully characterised using metagenome-assembled genomics, PCR–RFLP (PCR-Restriction Fragment Length Polymorphism) and MLST (MultiLocus Sequence Typing) analyses. This Wolbachia strain wPipSJ belongs to haplotype I, group wPip-III and supergroup B, clustering with other mosquito wPip strains, such as wPip PEL, wPip JHB, wPip Mol, and wAlbB; showing the southernmost distribution in America. The cytoplasmic incompatibility phenotype of this strain was revealed via crosses between wildtype (Wolbachia +) and antibiotic treated mosquito populations. The results of the tests with the bacterial agents suggest that Cx. quinquefasciatus naturally infected with wPipSJ is less susceptible to the pathogenic action of mosquitocidal bacterial strains when compared with the antibiotic-treated mosquito isoline, and is more susceptible to B. thuringiensis subsp. israelensis than to the other two mosquitocidal agents.
format	article
author	Leonardo M. Díaz-Nieto M. Florencia Gil J. Nicolás Lazarte M. Alejandra Perotti Corina M. Berón
author_facet	Leonardo M. Díaz-Nieto M. Florencia Gil J. Nicolás Lazarte M. Alejandra Perotti Corina M. Berón
author_sort	Leonardo M. Díaz-Nieto
title	Culex quinquefasciatus carrying Wolbachia is less susceptible to entomopathogenic bacteria
title_short	Culex quinquefasciatus carrying Wolbachia is less susceptible to entomopathogenic bacteria
title_full	Culex quinquefasciatus carrying Wolbachia is less susceptible to entomopathogenic bacteria
title_fullStr	Culex quinquefasciatus carrying Wolbachia is less susceptible to entomopathogenic bacteria
title_full_unstemmed	Culex quinquefasciatus carrying Wolbachia is less susceptible to entomopathogenic bacteria
title_sort	culex quinquefasciatus carrying wolbachia is less susceptible to entomopathogenic bacteria
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/4b879ef4070b4a4d952de83da06125a4
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Culex quinquefasciatus carrying Wolbachia is less susceptible to entomopathogenic bacteria

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