A systematic approach to inserting split inteins for Boolean logic gate engineering and basal activity reduction

Split inteins are powerful tools for designing synthetic split proteins. Here the authors use a mini-Mu transposon screen to map split sites, enabling the development of protein-based logic gates and fine control of protein activity.

Guardado en:

Detalles Bibliográficos
Autores principales:	Trevor Y. H. Ho, Alexander Shao, Zeyu Lu, Harri Savilahti, Filippo Menolascina, Lei Wang, Neil Dalchau, Baojun Wang
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/4b896aa452b543a29fce64b160991170
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:4b896aa452b543a29fce64b160991170
record_format	dspace
spelling	oai:doaj.org-article:4b896aa452b543a29fce64b1609911702021-12-02T14:25:16ZA systematic approach to inserting split inteins for Boolean logic gate engineering and basal activity reduction10.1038/s41467-021-22404-92041-1723https://doaj.org/article/4b896aa452b543a29fce64b1609911702021-04-01T00:00:00Zhttps://doi.org/10.1038/s41467-021-22404-9https://doaj.org/toc/2041-1723Split inteins are powerful tools for designing synthetic split proteins. Here the authors use a mini-Mu transposon screen to map split sites, enabling the development of protein-based logic gates and fine control of protein activity.Trevor Y. H. HoAlexander ShaoZeyu LuHarri SavilahtiFilippo MenolascinaLei WangNeil DalchauBaojun WangNature PortfolioarticleScienceQENNature Communications, Vol 12, Iss 1, Pp 1-12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Trevor Y. H. Ho Alexander Shao Zeyu Lu Harri Savilahti Filippo Menolascina Lei Wang Neil Dalchau Baojun Wang A systematic approach to inserting split inteins for Boolean logic gate engineering and basal activity reduction
description	Split inteins are powerful tools for designing synthetic split proteins. Here the authors use a mini-Mu transposon screen to map split sites, enabling the development of protein-based logic gates and fine control of protein activity.
format	article
author	Trevor Y. H. Ho Alexander Shao Zeyu Lu Harri Savilahti Filippo Menolascina Lei Wang Neil Dalchau Baojun Wang
author_facet	Trevor Y. H. Ho Alexander Shao Zeyu Lu Harri Savilahti Filippo Menolascina Lei Wang Neil Dalchau Baojun Wang
author_sort	Trevor Y. H. Ho
title	A systematic approach to inserting split inteins for Boolean logic gate engineering and basal activity reduction
title_short	A systematic approach to inserting split inteins for Boolean logic gate engineering and basal activity reduction
title_full	A systematic approach to inserting split inteins for Boolean logic gate engineering and basal activity reduction
title_fullStr	A systematic approach to inserting split inteins for Boolean logic gate engineering and basal activity reduction
title_full_unstemmed	A systematic approach to inserting split inteins for Boolean logic gate engineering and basal activity reduction
title_sort	systematic approach to inserting split inteins for boolean logic gate engineering and basal activity reduction
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/4b896aa452b543a29fce64b160991170
work_keys_str_mv	AT trevoryhho asystematicapproachtoinsertingsplitinteinsforbooleanlogicgateengineeringandbasalactivityreduction AT alexandershao asystematicapproachtoinsertingsplitinteinsforbooleanlogicgateengineeringandbasalactivityreduction AT zeyulu asystematicapproachtoinsertingsplitinteinsforbooleanlogicgateengineeringandbasalactivityreduction AT harrisavilahti asystematicapproachtoinsertingsplitinteinsforbooleanlogicgateengineeringandbasalactivityreduction AT filippomenolascina asystematicapproachtoinsertingsplitinteinsforbooleanlogicgateengineeringandbasalactivityreduction AT leiwang asystematicapproachtoinsertingsplitinteinsforbooleanlogicgateengineeringandbasalactivityreduction AT neildalchau asystematicapproachtoinsertingsplitinteinsforbooleanlogicgateengineeringandbasalactivityreduction AT baojunwang asystematicapproachtoinsertingsplitinteinsforbooleanlogicgateengineeringandbasalactivityreduction AT trevoryhho systematicapproachtoinsertingsplitinteinsforbooleanlogicgateengineeringandbasalactivityreduction AT alexandershao systematicapproachtoinsertingsplitinteinsforbooleanlogicgateengineeringandbasalactivityreduction AT zeyulu systematicapproachtoinsertingsplitinteinsforbooleanlogicgateengineeringandbasalactivityreduction AT harrisavilahti systematicapproachtoinsertingsplitinteinsforbooleanlogicgateengineeringandbasalactivityreduction AT filippomenolascina systematicapproachtoinsertingsplitinteinsforbooleanlogicgateengineeringandbasalactivityreduction AT leiwang systematicapproachtoinsertingsplitinteinsforbooleanlogicgateengineeringandbasalactivityreduction AT neildalchau systematicapproachtoinsertingsplitinteinsforbooleanlogicgateengineeringandbasalactivityreduction AT baojunwang systematicapproachtoinsertingsplitinteinsforbooleanlogicgateengineeringandbasalactivityreduction
_version_	1718391366258524160

A systematic approach to inserting split inteins for Boolean logic gate engineering and basal activity reduction

Ejemplares similares